BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,626	C→T	40.6%	A102T (GCA→ACA)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,626	0	C	T	40.6%	99.6 / 165.8	138	A102T (GCA→ACA)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base C (39/42); new base T (18/38); total (57/81)
Fisher's exact test for biased strand distribution p-value = 7.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 139 total aligned reads displayed.

CCGTGGTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAATCAGAC  >  minE/2677556‑2677694

   tGGTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGc                                                                   <  1:3319141/71‑1 (MQ=255)

   tGGTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGc                                                                   <  1:277561/71‑1 (MQ=255)

    ggTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGc                                                                   >  1:2607/1‑70 (MQ=255)

     gTAAAACAACAATGGCACCCCCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                                 >  1:1574034/1‑71 (MQ=255)

      tAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                                 >  1:754696/1‑70 (MQ=255)

        aaaCAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATa                                                               <  1:3196517/70‑1 (MQ=255)

        aaaCAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                                 >  1:3932891/1‑68 (MQ=255)

        aaaCAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                                 >  1:4106748/1‑68 (MQ=255)

        aaaCAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATAGCGGCaa                                                                 >  1:836039/1‑68 (MQ=255)

        aaaCAACAATGGCACAACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                                 >  1:779109/1‑68 (MQ=255)

         aacaacAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGc                                                             <  1:1221377/71‑1 (MQ=255)

         aacaacAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGc                                                             <  1:658914/71‑1 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTTATTTTGCATTGCGGCAATAGCg                                                            >  1:3952934/1‑70 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCg                                                            >  1:464135/1‑70 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCg                                                            >  1:2323063/1‑70 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCg                                                            >  1:2269919/1‑70 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCg                                                            >  1:859341/1‑70 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCg                                                            >  1:1437735/1‑70 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCg                                                            >  1:1282795/1‑70 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                           <  1:3941633/71‑1 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                           <  1:2333271/71‑1 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                           <  1:4144859/71‑1 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                           <  1:4245481/71‑1 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                                 >  1:4032761/1‑65 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGt                                                           <  1:1310649/71‑1 (MQ=255)

            aacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                           <  1:1889715/70‑1 (MQ=255)

               aaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACg                                                       <  1:4194457/71‑1 (MQ=255)

                aTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACg                                                       <  1:474521/70‑1 (MQ=255)

                   gCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGcat                                                    <  1:672268/70‑1 (MQ=255)

                   gCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGcat                                                    <  1:2155604/70‑1 (MQ=255)

                   gCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGcat                                                    <  1:1833630/70‑1 (MQ=255)

                     accaccATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGcatcat                                                 >  1:1702462/1‑71 (MQ=255)

                       caccaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGc                                               <  1:2074214/71‑1 (MQ=255)

                       caccaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGc                                               >  1:967541/1‑71 (MQ=255)

                         ccaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGc                                               >  1:1338948/1‑69 (MQ=255)

                         ccaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCg                                              >  1:351674/1‑70 (MQ=255)

                         ccaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGa                                             <  1:1071592/71‑1 (MQ=255)

                         ccaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGa                                             <  1:1967630/71‑1 (MQ=255)

                         ccaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGa                                             <  1:2387070/71‑1 (MQ=255)

                          caTCGGCAACGTTTTGTATCCAGGATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGaa                                            <  1:4229729/71‑1 (MQ=255)

                          caTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGaa                                            <  1:4125746/71‑1 (MQ=255)

                          caTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCa                                                                  <  1:1305113/49‑1 (MQ=255)

                          caTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGa                                             <  1:1066586/70‑1 (MQ=255)

                            tCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCg                                              <  1:1244091/67‑1 (MQ=255)

                            tCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCg                                              <  1:3877406/67‑1 (MQ=255)

                            tCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAAt                                                                >  1:1968684/1‑49 (MQ=255)

                             cGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATg                                         >  1:1054580/1‑71 (MQ=255)

                              ggCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGc                                        <  1:3229385/71‑1 (MQ=255)

                              ggCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGc                                        >  1:4064858/1‑71 (MQ=255)

                                   cGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGAt                                     >  1:937204/1‑69 (MQ=255)

                                    gTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGaaa                                           >  1:1783853/1‑62 (MQ=255)

                                    gTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGaaa                                           >  1:1722062/1‑62 (MQ=255)

                                    gTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGaaa                                           >  1:2558920/1‑62 (MQ=255)

                                    gTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGaaa                                           >  1:189345/1‑62 (MQ=255)

                                    gTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAg                                  >  1:2966639/1‑71 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGAt                                     <  1:3240791/67‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGAt                                     <  1:2809322/67‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGAt                                     <  1:1599824/67‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGAGGCAATAGCGTAACGCATCATGCGAAATGCGAt                                     <  1:3116563/67‑1 (MQ=255)

                                       ttGTATCCAGCATCAAGGTGATTTTTCATTGTGGCATTAGCGTAACGCATCATGCGAAATGCGAt                                     <  1:3767147/65‑1 (MQ=255)

                                        tGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttg                              >  1:3667125/1‑71 (MQ=255)

                                        tGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttg                              >  1:4206890/1‑71 (MQ=255)

                                        tGTATCAAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttg                              >  1:2898212/1‑71 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                          <  1:1501402/71‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                          <  1:1449575/71‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                          <  1:2795667/71‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                          <  1:2647759/71‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                          <  1:2573868/71‑1 (MQ=255)

                                             ccAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCa                                   >  1:239997/1‑61 (MQ=255)

                                              cAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAg                        >  1:3103447/1‑71 (MQ=255)

                                              cAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAg                        >  1:741323/1‑71 (MQ=255)

                                                gCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGt                      >  1:1971463/1‑71 (MQ=255)

                                                  aTCAAGGTGATTTTGCATTGCGGCAATAGCGTAACTCATCATGCGAAATGCGATCAGtttgtttg                          <  1:2446029/65‑1 (MQ=255)

                                                  aTCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgt                                 >  1:3719699/1‑58 (MQ=255)

                                                  aTCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgt                                 >  1:656690/1‑58 (MQ=255)

                                                  aTCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                          <  1:3181639/65‑1 (MQ=255)

                                                  aTCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                          <  1:3625282/65‑1 (MQ=255)

                                                     aaGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCttt                 <  1:1645689/71‑1 (MQ=255)

                                                     aaGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCttt                 <  1:840303/71‑1 (MQ=255)

                                                     aaGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCg                                       <  1:1078290/49‑1 (MQ=255)

                                                     aaGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCg                                       <  1:608615/49‑1 (MQ=255)

                                                      aGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttgttt                           <  1:1644015/60‑1 (MQ=255)

                                                      aGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttgttt                           <  1:2037506/60‑1 (MQ=255)

                                                      aGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTg                <  1:2572886/71‑1 (MQ=255)

                                                      aGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTg                <  1:3343958/71‑1 (MQ=255)

                                                       ggTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGa               >  1:1771944/1‑71 (MQ=255)

                                                       ggTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGa               >  1:2039905/1‑71 (MQ=255)

                                                       ggTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGa               >  1:1690852/1‑71 (MQ=255)

                                                       ggTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGa               <  1:2115553/71‑1 (MQ=255)

                                                             tttGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTc          >  1:3128687/1‑70 (MQ=255)

                                                                gCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAAt       <  1:2614845/70‑1 (MQ=255)

                                                                 cATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAATCa     <  1:112590/71‑1 (MQ=255)

                                                                  aTTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAAt       <  1:970657/68‑1 (MQ=255)

                                                                  aTTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAAt       <  1:2755884/68‑1 (MQ=255)

                                                                  aTTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAAt       <  1:2882679/68‑1 (MQ=255)

                                                                   ttGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttgt                             <  1:689015/45‑1 (MQ=255)

                                                                   ttGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttgt                             <  1:4187408/45‑1 (MQ=255)

                                                                   ttGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttgt                             <  1:868580/45‑1 (MQ=255)

                                                                   ttGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttgt                             <  1:3798546/45‑1 (MQ=255)

                                                                    tGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAATCAGa   >  1:2094704/1‑70 (MQ=255)

CCGTGGTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAATCAGAC  >  minE/2677556‑2677694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`