| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,687,159 | G→A | 44.0% | L374L (CTG→TTG) | pck ← | phosphoenolpyruvate carboxykinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,687,159 | 0 | G | A | 44.0% | 40.5 / 120.6 | 91 | L374L (CTG→TTG) | pck | phosphoenolpyruvate carboxykinase |
| Reads supporting (aligned to +/- strand): ref base G (13/38); new base A (22/18); total (35/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.09e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
CTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCC > minE/2687094‑2687226 | cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:561974/71‑1 (MQ=255)cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:750865/71‑1 (MQ=255) cAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgaga > 1:1133984/1‑70 (MQ=255) gTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGaaa > 1:1111586/1‑70 (MQ=255) gTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGaaa > 1:2895554/1‑70 (MQ=255) gTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAAc > 1:3754960/1‑71 (MQ=255) ccGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcg < 1:973381/70‑1 (MQ=255) ccGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcg < 1:3942382/70‑1 (MQ=255) ccGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcg < 1:3804580/70‑1 (MQ=255) ccGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACcgg > 1:2833800/1‑71 (MQ=255) ccGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACcgg > 1:2251693/1‑71 (MQ=255) ccGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACcgg > 1:1469361/1‑71 (MQ=255) ccGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACcgg > 1:1591338/1‑71 (MQ=255) ccGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACcgg > 1:1635870/1‑71 (MQ=255) ccAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCggcggc < 1:3340194/71‑1 (MQ=255) ccAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCggcggc < 1:2523970/71‑1 (MQ=255) ccAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCggcggc < 1:242808/71‑1 (MQ=255) tGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACg > 1:1219867/1‑71 (MQ=255) tGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAacac < 1:2419294/70‑1 (MQ=255) tGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcg < 1:3648060/54‑1 (MQ=255) gAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAg > 1:1772570/1‑71 (MQ=255) gAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAg > 1:2412177/1‑71 (MQ=255) gAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAg > 1:3020903/1‑71 (MQ=255) aaGCCAGAGAGGATGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAg < 1:3406057/70‑1 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGaaa > 1:3194362/1‑69 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAg > 1:3713969/1‑70 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAg > 1:2816823/1‑70 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAg > 1:224650/1‑70 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:907111/44‑1 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:3048064/44‑1 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:2298792/44‑1 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:3093941/44‑1 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:3881240/44‑1 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:3732947/44‑1 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:371440/44‑1 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgag < 1:1172932/44‑1 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCAt < 1:2962810/71‑1 (MQ=255) cAGAGAGGAAGTGATACTGTGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCAt > 1:1416880/1‑69 (MQ=255) cAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCAt > 1:3175741/1‑69 (MQ=255) gagagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:2433534/71‑1 (MQ=255) gagagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:2438668/71‑1 (MQ=255) gagagGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:3483622/71‑1 (MQ=255) gagagGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:1123489/71‑1 (MQ=255) gagagGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:2563812/71‑1 (MQ=255) agagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcggcgg < 1:2002297/49‑1 (MQ=255) agagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcag > 1:171/1‑69 (MQ=255) agagGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCaa > 1:2524481/1‑52 (MQ=255) agagGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCaa > 1:1008228/1‑52 (MQ=255) agagGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCaa > 1:2098748/1‑52 (MQ=255) agGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGt < 1:3034616/71‑1 (MQ=255) agGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGt < 1:797265/71‑1 (MQ=255) agGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGt < 1:1774483/71‑1 (MQ=255) gAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGa < 1:2034478/56‑1 (MQ=255) gTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGa < 1:1754654/71‑1 (MQ=255) gATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:464242/1‑68 (MQ=255) gATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:1524469/1‑68 (MQ=255) gATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:1377421/1‑68 (MQ=255) gATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:2097059/1‑68 (MQ=255) gATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:2075953/1‑68 (MQ=255) aCTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGc < 1:1029724/52‑1 (MQ=255) aCTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGc < 1:3828780/52‑1 (MQ=255) aCTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGc < 1:1898899/52‑1 (MQ=255) aCTGGGTTTGATCGGCAGTCAGGCGAGAAAACGGCGGCAACACGCCGAAAGc < 1:1054245/52‑1 (MQ=255) cTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGc < 1:2394248/51‑1 (MQ=255) cTGGGTTTGATCGGCAGTCAAGCGTGAAACCGGCGGCAACACGCCg < 1:1652683/46‑1 (MQ=255) cTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCg < 1:2569633/46‑1 (MQ=255) tGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATc < 1:3492057/53‑1 (MQ=255) gggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAcc > 1:416183/1‑71 (MQ=255) ggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCt > 1:3226000/1‑71 (MQ=255) ggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCt > 1:2725084/1‑71 (MQ=255) ggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCt > 1:4204229/1‑71 (MQ=255) tttGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:3006845/52‑1 (MQ=255) tttGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:3937958/71‑1 (MQ=255) tttGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:2967244/71‑1 (MQ=255) tttGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:1759179/71‑1 (MQ=255) tttGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCGGCAGTCAGGAAGATAACCTTa < 1:1607480/71‑1 (MQ=255) tttGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:4027535/71‑1 (MQ=255) tttGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:2657527/71‑1 (MQ=255) tttGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:2830531/71‑1 (MQ=255) tttGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:1118304/71‑1 (MQ=255) tttGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:1015387/71‑1 (MQ=255) tttGATCGACAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:231093/71‑1 (MQ=255) gATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGc < 1:719586/43‑1 (MQ=255) aTCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagca < 1:4241061/49‑1 (MQ=255) aTCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagca < 1:1783234/49‑1 (MQ=255) aTCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagca < 1:1321340/49‑1 (MQ=255) cAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTgg < 1:735127/71‑1 (MQ=255) aGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa > 1:3458700/1‑61 (MQ=255) aGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGc > 1:2984594/1‑71 (MQ=255) tCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAc < 1:752522/55‑1 (MQ=255) cAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGccc > 1:3177900/1‑70 (MQ=255) | CTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCC > minE/2687094‑2687226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |