| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,702,622 | G→T | 5.1% | V5F (GTC→TTC) | aroB → | 3‑dehydroquinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,702,622 | 0 | G | T | 5.1% | 447.8 / 4.4 | 134 | V5F (GTC→TTC) | aroB | 3‑dehydroquinate synthase |
| Reads supporting (aligned to +/- strand): ref base G (80/46); new base T (2/5); total (82/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
Only 100 of 141 total aligned reads displayed.
ATTCTGGCTTTATATACACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTAATGAACCAGCT > minE/2702553‑2702687 | aTTCTGGCTTTATATACACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGt > 1:1263573/1‑71 (MQ=255) cTGGCTTTATATACACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTGGCGTTATGGAGAGGATTGTCGt > 1:1136324/1‑71 (MQ=255) cTGGCTTTATATACACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGt > 1:823785/1‑71 (MQ=255) cTGGCTTTATATACACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGt > 1:3543789/1‑71 (MQ=255) cTGGCTTTATATACACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGt > 1:1405962/1‑71 (MQ=255) gCTTTATATACACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGt < 1:437124/68‑1 (MQ=255) tataCACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCgg > 1:2988782/1‑71 (MQ=255) ataCACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCggg > 1:1666058/1‑71 (MQ=255) ataCACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCggg > 1:2617620/1‑71 (MQ=255) ataCACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCggg > 1:2866963/1‑71 (MQ=255) ataCACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCggg > 1:3028142/1‑71 (MQ=255) ataCACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCggg > 1:3208772/1‑71 (MQ=255) ataCACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCggg > 1:3881089/1‑71 (MQ=255) taCACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCgtgg < 1:4078007/71‑1 (MQ=255) taCACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCggg > 1:1204948/1‑70 (MQ=255) acacTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTc > 1:3834678/1‑57 (MQ=255) acacTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTc > 1:3658923/1‑57 (MQ=255) cacTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTAct > 1:4214549/1‑62 (MQ=255) cacTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCgg > 1:192125/1‑67 (MQ=255) cacTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCgg > 1:1618304/1‑67 (MQ=255) cTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACg < 1:2582869/71‑1 (MQ=255) cTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACg > 1:2701072/1‑71 (MQ=255) cTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACg > 1:2834340/1‑71 (MQ=255) tCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTTTCGTTACTCTCGGGGAACg > 1:4127924/1‑70 (MQ=255) tCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTTTCGTTACTCTCGGGGAACg > 1:2956993/1‑70 (MQ=255) tCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTTTCGTTACTCTCGGGGAACGTAGt < 1:239004/71‑1 (MQ=255) tCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTTTCGTTACTCTCGGGGAACGTAGt < 1:3299018/71‑1 (MQ=255) tCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTTTCGTTACTCTCGGGGAACGTAGt < 1:609773/71‑1 (MQ=255) ggTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCa < 1:2688707/71‑1 (MQ=255) ggTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCa < 1:2212916/71‑1 (MQ=255) cAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAAtt < 1:2716340/70‑1 (MQ=255) cAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTa > 1:1562378/1‑71 (MQ=255) cAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTa < 1:3211970/71‑1 (MQ=255) gTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTActc > 1:1169875/1‑45 (MQ=255) gTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTAc > 1:1069498/1‑70 (MQ=255) gTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACACTCGGGGAACGTAGTTACCCAATTAc > 1:3651445/1‑70 (MQ=255) tAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGaa < 1:3330111/52‑1 (MQ=255) tAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGaa < 1:2496892/52‑1 (MQ=255) tAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCa > 1:1251441/1‑71 (MQ=255) tAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCa < 1:1747283/71‑1 (MQ=255) aaTTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:3705371/1‑71 (MQ=255) aaTTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:421722/1‑71 (MQ=255) aaTTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:901710/1‑71 (MQ=255) aaTTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:1494319/1‑71 (MQ=255) aaTTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:2263248/1‑71 (MQ=255) aTTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCaa > 1:3616098/1‑63 (MQ=255) aTTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCaa > 1:308113/1‑63 (MQ=255) aaGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGt > 1:2954659/1‑53 (MQ=255) aaGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTAcc > 1:359689/1‑65 (MQ=255) aaGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGc < 1:3974681/70‑1 (MQ=255) aaGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGc > 1:306986/1‑70 (MQ=255) aaGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCa > 1:1033434/1‑71 (MQ=255) aaGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCa > 1:1745584/1‑71 (MQ=255) aaGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCa > 1:3526245/1‑71 (MQ=255) aaGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCa > 1:2573341/1‑71 (MQ=255) aaGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCa > 1:233486/1‑71 (MQ=255) aGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACtctc > 1:2998497/1‑40 (MQ=255) aGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACtctc > 1:2935471/1‑40 (MQ=255) aGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACtctc > 1:477486/1‑40 (MQ=255) aGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACtctc > 1:1218070/1‑40 (MQ=255) aGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCAt > 1:855158/1‑71 (MQ=255) aGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCAt > 1:1085190/1‑71 (MQ=255) aGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCAt > 1:1400104/1‑71 (MQ=255) gATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCg > 1:2609927/1‑36 (MQ=255) gATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCAt > 1:1424469/1‑66 (MQ=255) aTGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTAccc < 1:3431471/51‑1 (MQ=255) aTGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTAccc < 1:2217258/51‑1 (MQ=255) aTGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTAccc < 1:1648901/51‑1 (MQ=255) tGTCGCGTTATGGAGATGATTGTCGTTACTCTCggg < 1:1970382/36‑1 (MQ=255) tGTCGCGTTATGGAGAGGATTGTCGTTACTCTCgggg < 1:2778335/37‑1 (MQ=255) tGTCGCGTTATGGAGAGGATTGTCGTTACTCTCgggg < 1:147793/37‑1 (MQ=255) tGTCGCGTTATGGAGAGGATTGTCGTTACTCTCggg < 1:3328249/36‑1 (MQ=255) tGTCGCGTTATGGAGAGGATTGTCGTTACTCTCggg < 1:765921/36‑1 (MQ=255) tCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGaa > 1:1788376/1‑37 (MQ=255) tCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGaa > 1:2412260/1‑37 (MQ=255) gcgTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGgtttgtt < 1:1864035/70‑1 (MQ=255) gcgTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGgtttgtt < 1:572505/70‑1 (MQ=255) gTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTaa < 1:131929/71‑1 (MQ=255) gTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTaa > 1:2918496/1‑71 (MQ=255) gTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTaa < 1:113978/71‑1 (MQ=255) ttATGGAGAGGATTGTCGTTACTCTCGGGGAACGTa < 1:1530285/36‑1 (MQ=255) tATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGgtttgttt < 1:3339369/67‑1 (MQ=255) aTGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTAATg > 1:2290663/1‑70 (MQ=255) ggAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTaa > 1:843418/1‑66 (MQ=255) ggAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTaa > 1:2206606/1‑66 (MQ=255) ggAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTaa > 1:4159438/1‑66 (MQ=255) ggAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTaa > 1:3874951/1‑66 (MQ=255) gagaGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTAATGAAc > 1:2184958/1‑70 (MQ=255) ggATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATc > 1:4167124/1‑49 (MQ=255) ggATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTAATGAACCAGCt > 1:3826692/1‑71 (MQ=255) ggATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTAATGAACCAGCt > 1:769695/1‑71 (MQ=255) ggATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTAATGAACCAGCt > 1:3577747/1‑71 (MQ=255) ttGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGgtttgttt < 1:1796395/56‑1 (MQ=255) ttGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGgtttgttt < 1:1706069/56‑1 (MQ=255) tGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGgtttgttt < 1:528133/55‑1 (MQ=255) gTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:1363361/1‑38 (MQ=255) gTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:3558698/1‑38 (MQ=255) gTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:3846606/1‑38 (MQ=255) gTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:741531/1‑38 (MQ=255) gTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCAt > 1:3637468/1‑38 (MQ=255) | ATTCTGGCTTTATATACACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTCGGGGAACGTAGTTACCCAATTACCATCGCATCTGGTTTGTTTAATGAACCAGCT > minE/2702553‑2702687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |