| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,732,642 | C→A | 49.0% | Q238K (CAA→AAA) | yheO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,732,642 | 0 | C | A | 49.0% | 80.1 / 121.1 | 104 | Q238K (CAA→AAA) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (14/39); new base A (29/22); total (43/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
CCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGTAGT > minE/2732575‑2732709 | ccTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:2184741/71‑1 (MQ=255) cTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCaa < 1:3225187/69‑1 (MQ=255) cTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCaa < 1:3631116/69‑1 (MQ=255) gAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGa > 1:4181992/1‑69 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGa > 1:2778799/1‑68 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt > 1:909676/1‑69 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt > 1:1818632/1‑69 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt > 1:1390659/1‑69 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt > 1:3370458/1‑69 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt > 1:1609150/1‑69 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt > 1:3928004/1‑69 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGa > 1:887033/1‑68 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGAt > 1:110207/1‑69 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGAt < 1:2335662/69‑1 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGAt > 1:882121/1‑69 (MQ=255) aCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAg < 1:2594655/71‑1 (MQ=255) aCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAg < 1:3366801/71‑1 (MQ=255) aCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAg < 1:3371603/71‑1 (MQ=255) cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:3328322/71‑1 (MQ=255) cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:4230101/71‑1 (MQ=255) aTCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTa < 1:2493770/71‑1 (MQ=255) ctcCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAAt > 1:92739/1‑71 (MQ=255) cAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGTGGCAAGACAAGTaa < 1:540684/67‑1 (MQ=255) aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGtt < 1:237983/71‑1 (MQ=255) aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGtt < 1:1339425/71‑1 (MQ=255) cacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGc < 1:1511002/71‑1 (MQ=255) acTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGcc < 1:1447975/71‑1 (MQ=255) acTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAAAGCGTTTTGcc < 1:335880/71‑1 (MQ=255) cTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCa < 1:3948805/71‑1 (MQ=255) cTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCa < 1:2898540/71‑1 (MQ=255) gTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATc > 1:2834066/1‑71 (MQ=255) tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAAt < 1:2172022/57‑1 (MQ=255) tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAAt < 1:1627304/57‑1 (MQ=255) tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAAt < 1:3834110/57‑1 (MQ=255) tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCg < 1:2150816/71‑1 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgtggt > 1:2850152/1‑71 (MQ=255) tctACATCCGCCAGTTCAAGAGCGGTCATTTCCAGGGGAAAGATAAGTAATGCGtt > 1:1481326/1‑56 (MQ=255) tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgtggtg < 1:4133762/68‑1 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCg > 1:2203248/1‑61 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg > 1:935504/1‑71 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg > 1:1932369/1‑71 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCg < 1:1879961/70‑1 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCg < 1:1634176/70‑1 (MQ=255) cGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGc > 1:388893/1‑69 (MQ=255) cAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGc < 1:1392216/70‑1 (MQ=255) cAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:928758/71‑1 (MQ=255) ttCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAAt < 1:903353/37‑1 (MQ=255) ttCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAAt < 1:3549239/37‑1 (MQ=255) ttCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAAt < 1:1713624/37‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg < 1:520617/70‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:706400/71‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:3513340/71‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg < 1:4169665/70‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg < 1:2029155/70‑1 (MQ=255) agagCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTa > 1:901157/1‑71 (MQ=255) cGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGc < 1:1937554/70‑1 (MQ=255) cGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGc < 1:1390804/70‑1 (MQ=255) cGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGc > 1:3681522/1‑70 (MQ=255) ggTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCaa < 1:811705/71‑1 (MQ=255) gTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtg < 1:2982886/43‑1 (MQ=255) gTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtg < 1:809971/43‑1 (MQ=255) gTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGAc < 1:2547407/48‑1 (MQ=255) gTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGAc < 1:74843/48‑1 (MQ=255) gTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGAc < 1:2483971/48‑1 (MQ=255) tGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg < 1:1588550/62‑1 (MQ=255) gATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAg > 1:1071233/1‑71 (MQ=255) gATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAg > 1:3180182/1‑71 (MQ=255) gATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAg > 1:1557705/1‑71 (MQ=255) gATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAg > 1:1935013/1‑71 (MQ=255) aTTTCCAGGGGCAAGATAATTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:3520049/55‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:1692850/55‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:1340933/55‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:362292/55‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:1203505/55‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:3958684/55‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:401639/55‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:1981314/55‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:2838651/55‑1 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCTATCGTGGTGACCGGGCCAGCATACGGTACGCAACa > 1:1333038/1‑69 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACa > 1:540832/1‑69 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACa > 1:135604/1‑69 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACa > 1:3177022/1‑69 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACa > 1:2994643/1‑69 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACa > 1:164835/1‑69 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:636562/1‑71 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:1949145/1‑71 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:2561418/1‑71 (MQ=255) aTTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:3275457/1‑71 (MQ=255) tttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGc > 1:4122609/1‑71 (MQ=255) tCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCg < 1:2835399/70‑1 (MQ=255) ccAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa > 1:1677087/1‑51 (MQ=255) ccAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa > 1:3549639/1‑51 (MQ=255) ccAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa > 1:442135/1‑51 (MQ=255) cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGag < 1:4080315/70‑1 (MQ=255) cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGag < 1:2110840/70‑1 (MQ=255) cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGag < 1:3161318/70‑1 (MQ=255) gggAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagtagt > 1:4116269/1‑71 (MQ=255) gggAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagtagt > 1:3618920/1‑71 (MQ=255) gggAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagtagt > 1:1153224/1‑71 (MQ=255) gggAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagtag > 1:1460560/1‑70 (MQ=255) | CCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGTAGT > minE/2732575‑2732709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |