| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 300,220 | G→A | 51.6% | L372L (CTG→CTA) | mdlB → | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 300,220 | 0 | G | A | 51.6% | ‑2.3 / 97.0 | 64 | L372L (CTG→CTA) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (22/9); new base A (12/21); total (34/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
CTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGC > minE/300150‑300281 | cTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTg < 1:4209172/71‑1 (MQ=255)cTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTg < 1:3760773/71‑1 (MQ=255) tCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCg > 1:2824477/1‑71 (MQ=255) tCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCg > 1:2763799/1‑71 (MQ=255) tCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCg > 1:443999/1‑71 (MQ=255) cgcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTg < 1:3224950/66‑1 (MQ=255) gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTTTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:2257009/71‑1 (MQ=255) gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:3003697/71‑1 (MQ=255) gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:3519378/71‑1 (MQ=255) gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:2011911/71‑1 (MQ=255) gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTCTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:3251920/71‑1 (MQ=255) aTGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCAt < 1:2310196/71‑1 (MQ=255) tGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:12521/67‑1 (MQ=255) tGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:111276/67‑1 (MQ=255) tGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:2141096/67‑1 (MQ=255) aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:36325/1‑71 (MQ=255) aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:1718234/1‑71 (MQ=255) aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:2541250/1‑71 (MQ=255) aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:768310/1‑71 (MQ=255) aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:3799045/1‑71 (MQ=255) aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAc > 1:2543159/1‑70 (MQ=255) gTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCa < 1:4204950/67‑1 (MQ=255) gTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCa < 1:391510/67‑1 (MQ=255) tGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGt < 1:2334836/68‑1 (MQ=255) cTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa < 1:503236/71‑1 (MQ=255) tAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa > 1:3476498/1‑70 (MQ=255) tAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa > 1:3245903/1‑70 (MQ=255) tAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa > 1:3301816/1‑70 (MQ=255) aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaaa < 1:3953914/70‑1 (MQ=255) aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaaa < 1:3197187/70‑1 (MQ=255) aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaa < 1:2976/69‑1 (MQ=255) gAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCa < 1:1089853/71‑1 (MQ=255) aaCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCAc > 1:3706919/1‑71 (MQ=255) cATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGATGGTCGGGCATACCGGCAGTGGCAAAAGCAccc < 1:3014939/71‑1 (MQ=255) aaTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCa > 1:4040436/1‑44 (MQ=255) aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:3623774/1‑61 (MQ=255) aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:990464/1‑61 (MQ=255) aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:2494536/1‑61 (MQ=255) aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:1802215/1‑61 (MQ=255) aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGc < 1:2904784/70‑1 (MQ=255) aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGc < 1:3571606/70‑1 (MQ=255) aTCTCTCAGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:2367394/1‑61 (MQ=255) tctctGTGCCTTCGCGCAATTTTTTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:1412274/1‑71 (MQ=255) tctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:727537/1‑71 (MQ=255) tctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:721540/1‑71 (MQ=255) tctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:2038429/1‑71 (MQ=255) tctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:2177623/1‑71 (MQ=255) tctctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCAcc > 1:157531/1‑62 (MQ=255) ctctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:546581/1‑71 (MQ=255) tctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAg < 1:1658079/68‑1 (MQ=255) tctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAg < 1:531431/68‑1 (MQ=255) tctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:565022/1‑70 (MQ=255) tctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:2409741/1‑70 (MQ=255) tctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:1399864/1‑70 (MQ=255) tgtgCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt < 1:666824/68‑1 (MQ=255) gCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGa > 1:1056925/1‑71 (MQ=255) gCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGa > 1:1252058/1‑71 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAt < 1:1731823/71‑1 (MQ=255) cgcgCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGc > 1:1107190/1‑71 (MQ=255) gcgcAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGc < 1:651575/70‑1 (MQ=255) gcgcAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGc < 1:2844454/70‑1 (MQ=255) cgcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCt < 1:1662521/70‑1 (MQ=255) gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTAt < 1:1195153/71‑1 (MQ=255) gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTAt < 1:406235/71‑1 (MQ=255) aaTTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTa < 1:1468447/71‑1 (MQ=255) ttGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCg > 1:1720423/1‑71 (MQ=255) gtgGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGc > 1:1221776/1‑70 (MQ=255) | CTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGC > minE/300150‑300281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |