| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,850,177 | G→A | 54.2% | Q286* (CAG→TAG) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,850,177 | 0 | G | A | 54.2% | 8.2 / 83.3 | 59 | Q286* (CAG→TAG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (12/15); new base A (10/22); total (22/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
GCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTT > minE/2850111‑2850233 | gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTg > 1:3618345/1‑70 (MQ=255)gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:1730780/1‑71 (MQ=255)gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:478974/1‑71 (MQ=255)gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:3338752/1‑71 (MQ=255) cGACTGTTCACGCCGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc < 1:4180325/70‑1 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc < 1:2646403/70‑1 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc < 1:3365216/70‑1 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc < 1:2030038/70‑1 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc < 1:2093533/70‑1 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc < 1:1003372/70‑1 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc < 1:3865448/70‑1 (MQ=255) aCTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAg > 1:3357277/1‑71 (MQ=255) aCTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAg > 1:2604285/1‑71 (MQ=255) aCTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAg > 1:807660/1‑71 (MQ=255) cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGc > 1:2193155/1‑67 (MQ=255) cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAAc > 1:642924/1‑70 (MQ=255) cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAAc > 1:2340251/1‑70 (MQ=255) aCGCAGCGTATTCAACGCCTGGCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:809738/71‑1 (MQ=255) aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACg < 1:2419821/70‑1 (MQ=255) aGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTTACGCTGTTTCGAGGCAACGAGatc < 1:2069313/71‑1 (MQ=255) aGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc < 1:2041719/71‑1 (MQ=255) aGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc < 1:1997396/71‑1 (MQ=255) cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca > 1:2403419/1‑71 (MQ=255) cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGatcca < 1:3899079/71‑1 (MQ=255) cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGatcca < 1:1025603/71‑1 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccatc > 1:1885775/1‑69 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccatc > 1:3791983/1‑69 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:982659/67‑1 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:309432/67‑1 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:4107742/67‑1 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:4140600/67‑1 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:255105/67‑1 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCg > 1:2166332/1‑71 (MQ=255) cTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCt < 1:4020319/71‑1 (MQ=255) cTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCt < 1:1640104/71‑1 (MQ=255) cTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCt < 1:2309024/71‑1 (MQ=255) gCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc < 1:289316/41‑1 (MQ=255) cTGTAACGTCTGGCTTGCAGCCTGACGCTATTGCGAGGCAACGAGatcc < 1:3923796/49‑1 (MQ=255) cTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGatcc < 1:2024357/49‑1 (MQ=255) tGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGatcc < 1:568725/48‑1 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgcgc < 1:3098108/57‑1 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaaa < 1:381688/70‑1 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaaa < 1:1836535/70‑1 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAg > 1:930882/1‑71 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGat > 1:217206/1‑44 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTc < 1:62665/67‑1 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTc < 1:3557462/67‑1 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTc < 1:282300/67‑1 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAg > 1:406261/1‑71 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCAGCTGCGCCTGTTGATTCAAAg > 1:1943040/1‑71 (MQ=255) aaCGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgc > 1:2305558/1‑71 (MQ=255) aCGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc > 1:1302830/1‑71 (MQ=255) tCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:551267/71‑1 (MQ=255) tCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:3047657/71‑1 (MQ=255) tCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:3731123/71‑1 (MQ=255) ctggctggCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:2540810/70‑1 (MQ=255) tggctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTc > 1:2327813/1‑60 (MQ=255) gctggcAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa > 1:1572663/1‑69 (MQ=255) gcAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGtt < 1:677940/70‑1 (MQ=255) | GCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTT > minE/2850111‑2850233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |