| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,864,584 | C→T | 43.2% | A295A (GCC→GCT) | hflK → | modulator for HflB protease specific for phage lambda cII repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,864,584 | 0 | C | T | 43.2% | 24.3 / 73.1 | 58 | A295A (GCC→GCT) | hflK | modulator for HflB protease specific for phage lambda cII repressor |
| Reads supporting (aligned to +/- strand): ref base C (18/14); new base T (12/13); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
CCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGC > minE/2864514‑2864654 | ccAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGcc > 1:2962320/1‑71 (MQ=255)ccAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGcc > 1:1303129/1‑71 (MQ=255) aaCGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCa < 1:307991/71‑1 (MQ=255) aaCGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGAGTGCGTACAAGGCTCa < 1:956800/71‑1 (MQ=255) cGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGCGGCGCGTGCGTACAAGGCCCa > 1:893705/1‑69 (MQ=255) cGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGa > 1:539393/1‑71 (MQ=255) cGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGa > 1:3290154/1‑71 (MQ=255) cGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGa > 1:3604256/1‑71 (MQ=255) tCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATc > 1:3079003/1‑70 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCa > 1:4199174/1‑67 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATcc > 1:2660885/1‑70 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCt > 1:1223257/1‑71 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCt > 1:3069391/1‑71 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCt > 1:2524120/1‑71 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCt > 1:2402754/1‑71 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCACAGACCATcc > 1:3889151/1‑70 (MQ=255) gCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTg > 1:1574345/1‑70 (MQ=255) gCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTg > 1:202904/1‑70 (MQ=255) gCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTg > 1:2173980/1‑70 (MQ=255) cGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCa < 1:1609307/70‑1 (MQ=255) cGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCa < 1:231814/70‑1 (MQ=255) cGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTg < 1:2655450/71‑1 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGAcc > 1:4215676/1‑51 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTg > 1:1070291/1‑70 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTg > 1:1655546/1‑70 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGa < 1:1057021/71‑1 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGa < 1:473278/71‑1 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGa < 1:1598328/71‑1 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGa < 1:258374/71‑1 (MQ=255) gcgcAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGgc > 1:198609/1‑71 (MQ=255) aCGTATCCTCGAAGAGGCGCTTGCGTACAAGGCTCAGACCATCCTgg < 1:2918080/47‑1 (MQ=255) aCGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTgg < 1:1674849/47‑1 (MQ=255) aCGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTgg < 1:232101/47‑1 (MQ=255) gTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGaa > 1:2768083/1‑47 (MQ=255) tCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAggg > 1:2083846/1‑52 (MQ=255) tCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAggg > 1:3747074/1‑52 (MQ=255) cTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTa > 1:629461/1‑70 (MQ=255) cGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTgg > 1:1746649/1‑38 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGgcgc > 1:1380049/1‑58 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGTCGCGCTTTGCTaa < 1:4132862/68‑1 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTaa < 1:317990/68‑1 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAAc > 1:3405928/1‑70 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAAc > 1:812898/1‑70 (MQ=255) ggCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAg < 1:1563102/47‑1 (MQ=255) ggCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAg < 1:4227141/47‑1 (MQ=255) gcgcGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGcc > 1:3232087/1‑71 (MQ=255) gcgtgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCgg < 1:3415849/71‑1 (MQ=255) cgtACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGaa > 1:2721679/1‑38 (MQ=255) tACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATaa < 1:3131309/71‑1 (MQ=255) tACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATaa < 1:2935985/71‑1 (MQ=255) tACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATaa < 1:2924023/71‑1 (MQ=255) tACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATaa < 1:2821036/71‑1 (MQ=255) cAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAg > 1:1420151/1‑71 (MQ=255) cAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAg > 1:4237597/1‑71 (MQ=255) aaGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTaa < 1:15956/50‑1 (MQ=255) aaGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTaa < 1:160281/50‑1 (MQ=255) aGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCgg < 1:3199334/60‑1 (MQ=255) aGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCgg < 1:3189391/60‑1 (MQ=255) aGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCgg < 1:2198765/60‑1 (MQ=255) gCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCcgc < 1:1166423/71‑1 (MQ=255) cccAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCcgcg < 1:1486054/71‑1 (MQ=255) ccAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCgcgc > 1:3181510/1‑71 (MQ=255) | CCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGC > minE/2864514‑2864654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |