| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,912,429 | C→T | 47.8% | A135T (GCG→ACG) | pyrI ← | aspartate carbamoyltransferase, regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,912,429 | 0 | C | T | 47.8% | 20.7 / 165.0 | 113 | A135T (GCG→ACG) | pyrI | aspartate carbamoyltransferase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base C (37/22); new base T (31/23); total (68/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTG > minE/2912364‑2912496 | gCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCa > 1:436346/1‑71 (MQ=255) gCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCa > 1:118673/1‑71 (MQ=255) gCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCa > 1:2370881/1‑71 (MQ=255) aattaattGGCTAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt < 1:369509/71‑1 (MQ=255) aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcg > 1:840397/1‑71 (MQ=255) aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc < 1:3969217/70‑1 (MQ=255) attGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcgc < 1:1585415/71‑1 (MQ=255) attGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcgc < 1:2135318/71‑1 (MQ=255) attGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc < 1:3084999/69‑1 (MQ=255) attGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc < 1:682148/69‑1 (MQ=255) ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGt < 1:1172031/70‑1 (MQ=255) gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGttt < 1:3569455/71‑1 (MQ=255) gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGttt < 1:861940/71‑1 (MQ=255) gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGttt < 1:2056069/71‑1 (MQ=255) ccAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGttt < 1:1075157/70‑1 (MQ=255) aGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTCCg < 1:1822571/71‑1 (MQ=255) gCACCATATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGCGCGTGATATCATTGGCGCGttt < 1:1878250/67‑1 (MQ=255) caccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCa < 1:4047246/71‑1 (MQ=255) accacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCa < 1:3997554/54‑1 (MQ=255) accacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCAc > 1:3302360/1‑71 (MQ=255) ccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATc < 1:3633670/52‑1 (MQ=255) ccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATc < 1:2828341/52‑1 (MQ=255) ccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGgcgc < 1:2077998/60‑1 (MQ=255) ccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGgcgc < 1:1757660/60‑1 (MQ=255) ccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGcgcg < 1:2896592/61‑1 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt > 1:2908922/1‑52 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt > 1:2046907/1‑52 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt > 1:2804427/1‑52 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt > 1:3334528/1‑52 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt > 1:455945/1‑52 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:3960362/1‑70 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:668605/1‑70 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:1143561/1‑70 (MQ=255) caTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:1784813/1‑45 (MQ=255) aTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGAGTGatat < 1:1455024/47‑1 (MQ=255) ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGcgcg < 1:219761/56‑1 (MQ=255) tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAg > 1:2450590/1‑71 (MQ=255) aTGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAtt > 1:2673116/1‑48 (MQ=255) aTGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGc > 1:4021626/1‑71 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:1529697/1‑38 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:2538891/1‑38 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:2582034/1‑38 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg > 1:2553916/1‑70 (MQ=255) aaaCTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCa > 1:900030/1‑40 (MQ=255) aaaCTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGAt > 1:1652956/1‑70 (MQ=255) aaaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:2671179/1‑50 (MQ=255) aaaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:4187364/1‑50 (MQ=255) aaaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:803448/1‑50 (MQ=255) aaaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:3890782/1‑50 (MQ=255) aaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGa < 1:4227492/71‑1 (MQ=255) aaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGa < 1:3138526/71‑1 (MQ=255) aaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGa < 1:1708498/71‑1 (MQ=255) aaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGa < 1:3047552/71‑1 (MQ=255) ctctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAg > 1:2395299/1‑61 (MQ=255) ctctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAg > 1:1326636/1‑61 (MQ=255) ctctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAg > 1:3134875/1‑61 (MQ=255) ctctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAg > 1:1934348/1‑61 (MQ=255) ctctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAg > 1:4060631/1‑61 (MQ=255) tttttCACAGTTTTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg > 1:3777811/1‑61 (MQ=255) tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg > 1:1912493/1‑61 (MQ=255) tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg > 1:3430056/1‑61 (MQ=255) tttttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCATTTTCGCACGGCAAAGCTGGATGAAACCg > 1:2019096/1‑71 (MQ=255) cacaGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCaa < 1:2313144/51‑1 (MQ=255) acaGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGTAAAGCTGGATGAAACCGGTTCg < 1:1420690/70‑1 (MQ=255) acaGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg < 1:3147821/55‑1 (MQ=255) acaGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg < 1:2519950/55‑1 (MQ=255) acaGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg < 1:1391685/55‑1 (MQ=255) acaGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg < 1:1307790/71‑1 (MQ=255) caGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg > 1:1901573/1‑70 (MQ=255) aGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg < 1:2475649/53‑1 (MQ=255) aGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGa > 1:324115/1‑55 (MQ=255) aGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa < 1:2909288/71‑1 (MQ=255) gTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg < 1:951669/52‑1 (MQ=255) gTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCCCACGGCAAAGCTg < 1:1359983/52‑1 (MQ=255) tATTTGCATTTGAGCGCGATATCATTGGCGCGttt > 1:1060371/1‑35 (MQ=255) tATTTGCATTTGAGCGCGATATCATTGGCGCGttt > 1:1194761/1‑35 (MQ=255) aTTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATgg > 1:1056139/1‑71 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGc > 1:3848329/1‑71 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGc > 1:3277686/1‑71 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGc > 1:253654/1‑71 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGc > 1:2809653/1‑71 (MQ=255) tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACg > 1:3507074/1‑40 (MQ=255) tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaaa > 1:4166431/1‑56 (MQ=255) tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaaa > 1:485950/1‑56 (MQ=255) tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaaa > 1:2709495/1‑56 (MQ=255) tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaaa > 1:31893/1‑56 (MQ=255) tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGc > 1:3319483/1‑66 (MQ=255) tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa > 1:2921050/1‑67 (MQ=255) cATTTGAGCGCTATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGa > 1:2286444/1‑70 (MQ=255) cATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGa > 1:1125212/1‑70 (MQ=255) tttGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATAc < 1:2796233/71‑1 (MQ=255) tttGAGCGCGATATCATTTGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGa < 1:3526899/68‑1 (MQ=255) tttGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATTGCTGa < 1:414035/68‑1 (MQ=255) tttGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGa < 1:3612187/68‑1 (MQ=255) ttGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGa < 1:603826/67‑1 (MQ=255) tGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAg > 1:2716006/1‑71 (MQ=255) tGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAg > 1:283400/1‑71 (MQ=255) tGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAg > 1:2183825/1‑71 (MQ=255) tGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAg > 1:3868776/1‑71 (MQ=255) gcgtGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTg < 1:3105036/71‑1 (MQ=255) | CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTG > minE/2912364‑2912496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |