| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,948,123 | T→C | 33.8% | T40A (ACC→GCC) | yjjB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,948,123 | 0 | T | C | 33.8% | 73.7 / 59.7 | 65 | T40A (ACC→GCC) | yjjB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (22/21); new base C (6/16); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCA > minE/2948055‑2948185 | ttttACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTa > 1:3825701/1‑70 (MQ=255) ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACt < 1:159483/71‑1 (MQ=255) cgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACt < 1:3044534/70‑1 (MQ=255) gccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACttt > 1:2101633/1‑71 (MQ=255) gccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACttt > 1:2432974/1‑71 (MQ=255) gccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACtt > 1:345474/1‑70 (MQ=255) aaTCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGAt < 1:1824204/71‑1 (MQ=255) tCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATgc < 1:11549/71‑1 (MQ=255) gCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCa < 1:2823278/70‑1 (MQ=255) ggTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGAt < 1:2269627/71‑1 (MQ=255) tataAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATAc < 1:3009252/71‑1 (MQ=255) aGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGa > 1:2110812/1‑55 (MQ=255) aGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAgc > 1:2315141/1‑71 (MQ=255) ccGATATGCCCGGGAACATAGGGATGACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAgcgc > 1:187783/1‑71 (MQ=255) ccGATATGCCCGGGAACATAGGGATAGCGGCCGCCACGGTAAACACttt > 1:60743/1‑49 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACttt > 1:3898108/1‑49 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACttt > 1:3699182/1‑49 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACttt > 1:3360509/1‑49 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAgcgc > 1:4011684/1‑71 (MQ=255) atatGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCa < 1:646339/57‑1 (MQ=255) atatGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCa < 1:598439/57‑1 (MQ=255) atatGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCa < 1:2955900/57‑1 (MQ=255) atGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCa > 1:953412/1‑71 (MQ=255) tGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCAt < 1:3213613/71‑1 (MQ=255) tGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCAt < 1:1400295/71‑1 (MQ=255) tGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCAt < 1:719794/71‑1 (MQ=255) gCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCAtt > 1:2665559/1‑71 (MQ=255) gCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGAGACCAtt > 1:2949392/1‑71 (MQ=255) cccGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCAtt < 1:3380490/70‑1 (MQ=255) cccGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTg < 1:1171236/71‑1 (MQ=255) ccGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGa < 1:3699965/71‑1 (MQ=255) ccGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTg < 1:1166379/70‑1 (MQ=255) ggAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATa > 1:3605087/1‑71 (MQ=255) ggAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGcc < 1:1154073/47‑1 (MQ=255) ggAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGcc < 1:911940/47‑1 (MQ=255) gAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGcc < 1:2164667/46‑1 (MQ=255) aaCATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATAcc < 1:1733726/71‑1 (MQ=255) aCATAGTGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCa < 1:2635669/71‑1 (MQ=255) aCATAGGGCTAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCa < 1:4145236/71‑1 (MQ=255) aCATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCa < 1:2701189/71‑1 (MQ=255) aCATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCa < 1:115949/45‑1 (MQ=255) cATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCaa > 1:283662/1‑71 (MQ=255) aTAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCATATACCAGCGCGACCATTGAATACCAAt < 1:1637545/71‑1 (MQ=255) aTAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAAt < 1:2337674/71‑1 (MQ=255) aTAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTAcc > 1:3042815/1‑71 (MQ=255) aTAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTAc > 1:3175010/1‑70 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATAcc < 1:2118135/61‑1 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATAcc < 1:2426758/61‑1 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCg < 1:2526961/71‑1 (MQ=255) aCGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAAt < 1:4114021/62‑1 (MQ=255) aCGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:3727008/1‑65 (MQ=255) aCGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTa > 1:340326/1‑66 (MQ=255) ggCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc > 1:3697277/1‑69 (MQ=255) ggCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc > 1:2237284/1‑69 (MQ=255) ggCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc > 1:3042671/1‑69 (MQ=255) ccgccACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCaa < 1:1740002/57‑1 (MQ=255) cgccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa < 1:2740876/71‑1 (MQ=255) cgccACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa < 1:1862890/71‑1 (MQ=255) cgccACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa < 1:538108/71‑1 (MQ=255) cgccACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa < 1:114952/71‑1 (MQ=255) gccACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATg > 1:932892/1‑57 (MQ=255) cACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCg < 1:3428277/61‑1 (MQ=255) cGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc > 1:531790/1‑61 (MQ=255) cGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc > 1:1258136/1‑61 (MQ=255) cGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc > 1:933366/1‑61 (MQ=255) | TTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCA > minE/2948055‑2948185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |