| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,986,542 | A→G | 33.1% | L19L (TTG→CTG) | arcA ← | DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,986,542 | 0 | A | G | 33.1% | 157.5 / 132.3 | 133 | L19L (TTG→CTG) | arcA | DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA |
| Reads supporting (aligned to +/- strand): ref base A (53/36); new base G (25/19); total (78/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
ATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAA > minE/2986477‑2986610 | aTGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgt < 1:1260509/69‑1 (MQ=255) cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc < 1:1012706/71‑1 (MQ=255) cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc < 1:2889002/71‑1 (MQ=255) cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc > 1:2414027/1‑71 (MQ=255) cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc > 1:1254960/1‑71 (MQ=255) tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgt < 1:2506291/71‑1 (MQ=255) ttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtg > 1:4146511/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCgtgt > 1:2003125/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCgtgt > 1:1508527/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt > 1:2912607/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt > 1:535069/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt > 1:4257997/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt > 1:4222865/1‑71 (MQ=255) cgcCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAcc < 1:2728025/71‑1 (MQ=255) cgcCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAcc < 1:4038738/71‑1 (MQ=255) cATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAAc < 1:1252419/71‑1 (MQ=255) cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCg > 1:3896217/1‑71 (MQ=255) cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCg > 1:1879309/1‑71 (MQ=255) cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCg > 1:385168/1‑71 (MQ=255) cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCg > 1:1761605/1‑71 (MQ=255) cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCg > 1:362214/1‑71 (MQ=255) tGTCGCTTCGAAAAGATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc > 1:3573879/1‑69 (MQ=255) gTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgt < 1:802745/50‑1 (MQ=255) tCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCa < 1:2174283/63‑1 (MQ=255) tCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgt < 1:1411516/49‑1 (MQ=255) tCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc < 1:1082136/67‑1 (MQ=255) tCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc < 1:1556739/67‑1 (MQ=255) gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTTTTGCGTGTTACCAAc > 1:3927043/1‑63 (MQ=255) gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAAc > 1:4012571/1‑63 (MQ=255) gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTc < 1:2431931/71‑1 (MQ=255) gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTc < 1:2747800/71‑1 (MQ=255) gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTc < 1:4137939/71‑1 (MQ=255) ttCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAcc < 1:3508607/58‑1 (MQ=255) ttCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAcc < 1:1416960/58‑1 (MQ=255) ttCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAcc < 1:3126116/58‑1 (MQ=255) ttCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCaa > 1:1776439/1‑71 (MQ=255) ttCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCaa > 1:1798502/1‑71 (MQ=255) tCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAAc > 1:2609941/1‑71 (MQ=255) tCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAAc > 1:3049154/1‑71 (MQ=255) tCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAAc > 1:3978835/1‑71 (MQ=255) tCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGAGTGTTACCAACTCGTCTTCAAc > 1:3687572/1‑71 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAcaa > 1:3367846/1‑55 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCaa > 1:3514148/1‑58 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCaa > 1:2580208/1‑58 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCaa > 1:3736527/1‑58 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCaa > 1:2084470/1‑58 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTc > 1:2244158/1‑64 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTc > 1:3323799/1‑64 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAc > 1:2143720/1‑70 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAc > 1:3278879/1‑70 (MQ=255) aaCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:4240352/1‑69 (MQ=255) aCATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTc < 1:4108514/56‑1 (MQ=255) aCATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTc < 1:609098/56‑1 (MQ=255) aCATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTc < 1:164319/56‑1 (MQ=255) aCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:4065612/1‑68 (MQ=255) catcatAGGCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATATGa < 1:2970128/71‑1 (MQ=255) catcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGa < 1:954687/71‑1 (MQ=255) catcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGa < 1:3907199/71‑1 (MQ=255) tcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAt < 1:525396/71‑1 (MQ=255) catAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtg > 1:3574332/1‑71 (MQ=255) catAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGa > 1:3962559/1‑63 (MQ=255) catAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGa > 1:771100/1‑63 (MQ=255) catAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtg > 1:3353330/1‑71 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCaa > 1:3125807/1‑48 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCaa > 1:2878593/1‑48 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtgt > 1:3243923/1‑71 (MQ=255) tAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtgtg < 1:1001504/71‑1 (MQ=255) tAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtgtg < 1:2171697/71‑1 (MQ=255) tAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtg > 1:2137819/1‑69 (MQ=255) ttCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGa < 1:647412/56‑1 (MQ=255) ttCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGa < 1:3109167/56‑1 (MQ=255) tCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGc > 1:3487651/1‑66 (MQ=255) ccGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGt < 1:4070094/70‑1 (MQ=255) ccGCTTCGAAAATACTTTTCAGAGTGTTGCGTGTTACCAACTCGCCTTCAACGATAAGAATGTGCGGGGt < 1:468796/70‑1 (MQ=255) cGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCg > 1:3019670/1‑65 (MQ=255) cGCTTCGAAAATACTTTACAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCg > 1:3453400/1‑65 (MQ=255) gCTTCGAAAATACTTTTCAGCGTGTTGCGTTTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTg < 1:2909852/71‑1 (MQ=255) gCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTg < 1:3551337/71‑1 (MQ=255) cTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGc < 1:1145716/71‑1 (MQ=255) tCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCggg > 1:3924459/1‑63 (MQ=255) cGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCaa > 1:3214948/1‑45 (MQ=255) cGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCaa > 1:636486/1‑45 (MQ=255) cGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCaa > 1:2436740/1‑45 (MQ=255) cGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCggg > 1:4031658/1‑62 (MQ=255) cGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCggg > 1:2533522/1‑62 (MQ=255) aaaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGttt < 1:2514143/71‑1 (MQ=255) aTACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTg < 1:1725185/62‑1 (MQ=255) tACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGt < 1:1832718/66‑1 (MQ=255) tACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGt < 1:3902776/66‑1 (MQ=255) tACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGt < 1:2754082/66‑1 (MQ=255) cTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCgggg < 1:2599490/55‑1 (MQ=255) cTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCgggg < 1:877584/55‑1 (MQ=255) cTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCgggg < 1:2904358/55‑1 (MQ=255) cTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGttt > 1:3960444/1‑66 (MQ=255) cTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGttt > 1:7138/1‑66 (MQ=255) cTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGAGCgggg < 1:10123/55‑1 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCt > 1:1168284/1‑70 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCt > 1:2144316/1‑70 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCt > 1:2679583/1‑70 (MQ=255) cAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTaaa > 1:2807042/1‑71 (MQ=255) | ATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAA > minE/2986477‑2986610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |