| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 138,172 | A→G | 52.8% | N665N (AAT→AAC) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 138,172 | 0 | A | G | 52.8% | 13.4 / 102.0 | 72 | N665N (AAT→AAC) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (19/15); new base G (22/16); total (41/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
CAGTCCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACATA > minE/138106‑138237 | cagtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTg > 1:1292232/1‑71 (MQ=255) agtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGATAGAAACTTGACGGCTGTTATCGCTGTTTg < 1:3499757/70‑1 (MQ=255) agtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTg < 1:3218643/70‑1 (MQ=255) agtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTg < 1:4092560/70‑1 (MQ=255) agtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGc < 1:3928179/71‑1 (MQ=255) gtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:4103344/71‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGa < 1:4170510/70‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGa < 1:3821705/70‑1 (MQ=255) aCCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGaaa > 1:3498025/1‑71 (MQ=255) cGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGa < 1:326707/67‑1 (MQ=255) cGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGa < 1:3948405/67‑1 (MQ=255) cGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGa < 1:1681010/67‑1 (MQ=255) gCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTATGCAGAAAtt > 1:533748/1‑70 (MQ=255) gCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTg < 1:909614/71‑1 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGaaa > 1:3161689/1‑67 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGaaa > 1:2758349/1‑67 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGaaa > 1:2428858/1‑67 (MQ=255) tGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:156198/1‑71 (MQ=255) cAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGaa > 1:3301215/1‑67 (MQ=255) cAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTg > 1:3589007/1‑71 (MQ=255) cAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTg > 1:4104627/1‑71 (MQ=255) cAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTg > 1:3238631/1‑71 (MQ=255) cAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTg > 1:1748220/1‑71 (MQ=255) cAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTg > 1:1314292/1‑71 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCa > 1:3048136/1‑56 (MQ=255) aCAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAg > 1:2345388/1‑71 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGa < 1:1341136/59‑1 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGa < 1:3498914/59‑1 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGa < 1:1515305/59‑1 (MQ=255) aaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGa < 1:2529611/58‑1 (MQ=255) caccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTccc > 1:402022/1‑71 (MQ=255) ccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCa < 1:2064500/71‑1 (MQ=255) cGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGt > 1:4139003/1‑61 (MQ=255) cGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGt > 1:843812/1‑61 (MQ=255) ggTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGt < 1:2411293/70‑1 (MQ=255) ggTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGt < 1:2319205/70‑1 (MQ=255) tGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGc < 1:3923339/37‑1 (MQ=255) tGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAAtt < 1:1108859/44‑1 (MQ=255) tGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGAc > 1:460526/1‑71 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACt > 1:3000359/1‑70 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCAt > 1:903193/1‑63 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCAt > 1:4135773/1‑63 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCAt > 1:1689442/1‑63 (MQ=255) cTGAGAGAAACTTGACGGATGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCAt > 1:1093435/1‑63 (MQ=255) tGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGtt < 1:1994358/56‑1 (MQ=255) tGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGtt < 1:31212/56‑1 (MQ=255) agagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCa < 1:4005445/69‑1 (MQ=255) agagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa < 1:1009418/71‑1 (MQ=255) agagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa < 1:495778/71‑1 (MQ=255) agagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa < 1:2826117/71‑1 (MQ=255) agaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGc > 1:2358730/1‑71 (MQ=255) agaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGc > 1:3768169/1‑71 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGc < 1:2266926/70‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCa > 1:4098461/1‑56 (MQ=255) gaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCa > 1:1658535/1‑56 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGc > 1:2083495/1‑70 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCa > 1:770033/1‑71 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCa > 1:419195/1‑71 (MQ=255) gACGTCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:4072477/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATaa > 1:1193379/1‑71 (MQ=255) aCGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCa < 1:1685743/48‑1 (MQ=255) aCGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCa < 1:2995454/48‑1 (MQ=255) ggCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATg > 1:2943952/1‑48 (MQ=255) cTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAAcccc > 1:4105044/1‑70 (MQ=255) aTCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCa > 1:4127035/1‑39 (MQ=255) aTCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATaa > 1:101181/1‑61 (MQ=255) aTCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACa > 1:601597/1‑70 (MQ=255) aTCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACa > 1:402950/1‑70 (MQ=255) aTCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCa < 1:2356870/49‑1 (MQ=255) aTCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACa > 1:181454/1‑70 (MQ=255) aTCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACa > 1:4146025/1‑70 (MQ=255) cGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACATa > 1:4008417/1‑70 (MQ=255) | CAGTCCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACATA > minE/138106‑138237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |