| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 498,441 | C→T | 63.6% | G24S (GGT→AGT) | galE ← | UDP‑galactose‑4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 498,441 | 0 | C | T | 63.6% | 143.9 / 171.1 | 155 | G24S (GGT→AGT) | galE | UDP‑galactose‑4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (45/11); new base T (51/47); total (97/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 156 total aligned reads displayed.
GCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCAT > minE/498375‑498510 | gCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGttt < 1:2129348/71‑1 (MQ=255)gCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGttt < 1:1288222/71‑1 (MQ=255)gCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGttt < 1:2927345/71‑1 (MQ=255) cTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtttt < 1:1467982/71‑1 (MQ=255) gATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTg < 1:2215895/69‑1 (MQ=255) gATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTg > 1:2470091/1‑69 (MQ=255) tAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAg > 1:774819/1‑70 (MQ=255) tAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt < 1:243357/71‑1 (MQ=255) aaCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa > 1:762001/1‑68 (MQ=255) aaCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa > 1:2023554/1‑68 (MQ=255) aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTaa > 1:3658529/1‑71 (MQ=255) aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTa < 1:2322295/70‑1 (MQ=255) aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTaa < 1:1941562/71‑1 (MQ=255) aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTaa < 1:3048310/71‑1 (MQ=255) ggCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGt < 1:2404745/66‑1 (MQ=255) gCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGt < 1:2639009/57‑1 (MQ=255) tACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtttt > 1:2433885/1‑56 (MQ=255) tACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtttt > 1:254794/1‑56 (MQ=255) tACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtttt > 1:399288/1‑56 (MQ=255) aCGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGcaca > 1:383384/1‑69 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAg > 1:3156950/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAg > 1:756181/1‑71 (MQ=255) gCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAgg < 1:17016/71‑1 (MQ=255) gCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAgg < 1:1644046/71‑1 (MQ=255) gCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAgg < 1:3455411/71‑1 (MQ=255) gCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAgg < 1:2473436/71‑1 (MQ=255) gCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAgg < 1:207714/71‑1 (MQ=255) gCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:3864423/1‑70 (MQ=255) tGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGt > 1:908670/1‑48 (MQ=255) tGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa > 1:1250373/1‑54 (MQ=255) tGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa > 1:2565144/1‑54 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTaa > 1:3957433/1‑56 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTaa > 1:405696/1‑56 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTaa > 1:159604/1‑56 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTAt < 1:2159961/70‑1 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTAt < 1:4069814/70‑1 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTAt > 1:553987/1‑70 (MQ=255) gcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTa < 1:3204987/54‑1 (MQ=255) gcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTa < 1:490628/54‑1 (MQ=255) gcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTa < 1:726558/54‑1 (MQ=255) gcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa < 1:1913355/71‑1 (MQ=255) gcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa < 1:1198401/71‑1 (MQ=255) cTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa < 1:1719652/70‑1 (MQ=255) cTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTAt < 1:609678/64‑1 (MQ=255) tGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACt < 1:3172752/67‑1 (MQ=255) tGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACt < 1:1424833/67‑1 (MQ=255) tGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACt < 1:2066520/67‑1 (MQ=255) tGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGcaca > 1:2599546/1‑56 (MQ=255) tGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGcaca > 1:532055/1‑56 (MQ=255) ttACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTc > 1:2057265/1‑67 (MQ=255) ttACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTc > 1:752860/1‑67 (MQ=255) ttACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTc > 1:1162908/1‑67 (MQ=255) ttACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt < 1:3122987/45‑1 (MQ=255) aCAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATg > 1:2936161/1‑70 (MQ=255) aCAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTc > 1:2214547/1‑65 (MQ=255) cAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:1480535/1‑60 (MQ=255) cAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:559277/1‑60 (MQ=255) cAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTa < 1:2827348/71‑1 (MQ=255) cAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTa < 1:3898878/71‑1 (MQ=255) ggTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCg > 1:3263078/1‑71 (MQ=255) gTTATCAAGATTGATGACATCATGACTGTTTTGCAGTa > 1:134538/1‑38 (MQ=37) gTTATCAAGAATGATGACATCATGACTGTTTTGCAGTaa > 1:4199931/1‑39 (MQ=255) gTTATCAAGAATGATGACATCATGACTGTTTTGCAGTaa > 1:2257974/1‑39 (MQ=255) gTTATCAAGAATGATGACATCATGACTGTTTTGCAGTaa > 1:3552511/1‑39 (MQ=255) gTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGc < 1:2176953/71‑1 (MQ=255) gTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGc < 1:1203390/71‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGc < 1:2315565/70‑1 (MQ=255) ttATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGc > 1:3172022/1‑70 (MQ=255) ttATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:375086/71‑1 (MQ=255) tATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTa > 1:3432403/1‑71 (MQ=255) tATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTa > 1:1372483/1‑71 (MQ=255) aaGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGt > 1:4014418/1‑45 (MQ=255) aaGAATGATGACATCATGACTGTTTTGCAGTAATAGCACACAGGTATGACTTCCAATGTAACCGCTaccac > 1:1800696/1‑71 (MQ=255) aGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:1174550/65‑1 (MQ=255) gAATGATGACATCATGACCGTTTTGCAGTAATTGca > 1:2789229/1‑36 (MQ=255) gAATGATGACATCATGACCGTTTTGCAGTAATTGca > 1:2563964/1‑36 (MQ=255) gAATGATGACATCATGACCGTTTTGCAGTAATTGca > 1:2318644/1‑36 (MQ=255) tgatgaCATCATGACTGTTTTGCAGTAATTGCACACAg < 1:262410/38‑1 (MQ=255) tgatgaCATCATGACTGTTTTGCAGTAATTGCACACAg < 1:689298/38‑1 (MQ=255) tgatgaCATCATGACTGTTTTGCAGTAATTGCACACAg < 1:2797090/38‑1 (MQ=255) atgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:2854356/1‑61 (MQ=255) atgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:2584455/1‑61 (MQ=255) atgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:3969833/1‑61 (MQ=255) tgaCATCATGACTGTTTTGCAGTAATTGCACACAg < 1:582022/35‑1 (MQ=255) tgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTa < 1:1509813/52‑1 (MQ=255) tgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc > 1:3088543/1‑70 (MQ=255) aCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAg < 1:2034779/71‑1 (MQ=255) catGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAct > 1:2900462/1‑71 (MQ=255) catGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAct > 1:2743314/1‑71 (MQ=255) atGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:3669126/51‑1 (MQ=255) atGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActc > 1:1114090/1‑71 (MQ=255) atGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActc > 1:130988/1‑71 (MQ=255) atGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActc > 1:677641/1‑71 (MQ=255) atGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActc > 1:4154197/1‑71 (MQ=255) gACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActct > 1:1059545/1‑70 (MQ=255) gACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActct > 1:2143130/1‑70 (MQ=255) gACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActct > 1:2941660/1‑70 (MQ=255) gACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActct > 1:309919/1‑70 (MQ=255) gACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActct > 1:3600664/1‑70 (MQ=255) ccGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCAt < 1:3207281/71‑1 (MQ=255) | GCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCAT > minE/498375‑498510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |