| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 507,425 | C→A | 32.2% | A71D (GCT→GAT) | ybhH → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 507,425 | 0 | C | A | 32.2% | 126.0 / 39.6 | 62 | A71D (GCT→GAT) | ybhH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (22/20); new base A (14/6); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01 | |||||||||||
ACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTGCGG > minE/507356‑507494 | aCGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:3811456/71‑1 (MQ=255) gTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGAt < 1:3162020/71‑1 (MQ=255) gTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGAt < 1:1451332/71‑1 (MQ=255) ttGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGAt < 1:684528/68‑1 (MQ=255) ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa > 1:2951130/1‑71 (MQ=255) ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa > 1:1318991/1‑71 (MQ=255) ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa > 1:1654438/1‑71 (MQ=255) ggcggTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGtgatga > 1:1344417/1‑62 (MQ=255) tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:522630/55‑1 (MQ=255) aaTCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGtt > 1:1824407/1‑71 (MQ=255) cTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:3436037/48‑1 (MQ=255) cTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:1378931/48‑1 (MQ=255) cTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTgcg > 1:205680/1‑69 (MQ=255) gACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTgc > 1:396687/1‑66 (MQ=255) gACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTgc > 1:4046476/1‑66 (MQ=255) gACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTgc > 1:1307399/1‑66 (MQ=255) gACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTgc > 1:1197983/1‑66 (MQ=255) ccAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTa < 1:3043223/71‑1 (MQ=255) tAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCg > 1:854041/1‑71 (MQ=255) tAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCg < 1:606333/71‑1 (MQ=255) aaaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATc > 1:4227718/1‑69 (MQ=255) aaaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt > 1:107680/1‑71 (MQ=255) aaaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt > 1:2387103/1‑71 (MQ=255) aaaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt > 1:3117712/1‑71 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTgatgat < 1:1054289/41‑1 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTgatgat < 1:3735250/41‑1 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTgatgat < 1:3553378/41‑1 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTgatgat < 1:3384362/41‑1 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTgatgat < 1:1255331/41‑1 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGtt > 1:3909295/1‑71 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGtt > 1:3052838/1‑71 (MQ=255) gTCGCCATTATTAGCCGTTCCAGCGATCCGTGTgatgat < 1:3653091/39‑1 (MQ=38) tCGCCATTATTTGCCGTTCCAGCGATCGGCGTGCTGATGTCGATTATc < 1:1486169/48‑1 (MQ=255) tCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt < 1:1963732/71‑1 (MQ=255) gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:1304063/1‑69 (MQ=255) gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:3734907/1‑69 (MQ=255) cATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc < 1:3582269/71‑1 (MQ=255) attattAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCt < 1:2747593/44‑1 (MQ=255) ttattaGTCGTTCCATCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa > 1:2010327/1‑71 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa > 1:2462343/1‑71 (MQ=255) tattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGt > 1:4017429/1‑44 (MQ=255) tattaGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAAc > 1:68854/1‑71 (MQ=255) tattaGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAAc > 1:3931475/1‑71 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACg > 1:1297519/1‑71 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGATGATGTCGAt > 1:685385/1‑36 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGATGATGTCGAt > 1:2500025/1‑36 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTc > 1:2592257/1‑61 (MQ=255) attaGCAGTTCCAGCGATCCGCGTGATGATGTCGAt > 1:247069/1‑36 (MQ=37) gCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTc < 1:2994921/71‑1 (MQ=255) gCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTc < 1:1658756/71‑1 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:3762596/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:3223221/1‑70 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa < 1:3186730/71‑1 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa < 1:3430225/71‑1 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa < 1:3425305/71‑1 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa < 1:374307/71‑1 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa < 1:4179364/71‑1 (MQ=255) gCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACg > 1:3269116/1‑71 (MQ=255) gATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa > 1:1329040/1‑54 (MQ=255) gATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa > 1:2951345/1‑54 (MQ=255) gATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa > 1:1013734/1‑54 (MQ=255) gATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa > 1:1645447/1‑54 (MQ=255) gATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa > 1:1798406/1‑54 (MQ=255) tCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTa < 1:3767065/71‑1 (MQ=255) tCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTa < 1:3196849/71‑1 (MQ=255) tCCGCGTGCTGATGTCGATTATCTGTTTGCGCAAGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTa < 1:1564407/71‑1 (MQ=255) cgTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTg < 1:3103279/71‑1 (MQ=255) cgTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTg < 1:1941655/71‑1 (MQ=255) cgTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTg < 1:3330119/71‑1 (MQ=255) cTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTGCgg > 1:3248158/1‑70 (MQ=255) | ACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTGCGG > minE/507356‑507494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |