| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 581,876 | G→A | 24.7% | P638P (CCG→CCA) | clpA → | ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 581,876 | 0 | G | A | 24.7% | 164.5 / 54.5 | 85 | P638P (CCG→CCA) | clpA | ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity |
| Reads supporting (aligned to +/- strand): ref base G (32/32); new base A (7/14); total (39/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
TCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGG > minE/581808‑581946 | tCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCgg > 1:458860/1‑70 (MQ=255)tCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCgg > 1:1290599/1‑70 (MQ=255) cATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAAt < 1:1815843/71‑1 (MQ=255) tGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTcc > 1:2466040/1‑71 (MQ=255) tGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTcc > 1:420277/1‑71 (MQ=255) tGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTcc > 1:3220148/1‑71 (MQ=255) ggTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCg > 1:560108/1‑71 (MQ=255) gTCTTATCCACCAGGATAATAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCg > 1:4109279/1‑70 (MQ=255) gTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCg > 1:2274362/1‑70 (MQ=255) cTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTaa > 1:2234994/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCg > 1:588943/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCg > 1:2380099/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCg > 1:1365201/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCg > 1:1799867/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCg > 1:2677859/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCg > 1:3719387/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCg > 1:4180253/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAA‑AAGATCTTTACACCGGAATTCCGTAACCGt > 1:1972853/1‑71 (MQ=255) tCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGt < 1:501135/71‑1 (MQ=255) tCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGt < 1:3528878/71‑1 (MQ=255) tCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCGt < 1:894570/71‑1 (MQ=255) ccaccaGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGt < 1:780786/70‑1 (MQ=255) caccaGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGtct > 1:4056054/1‑71 (MQ=255) ccaGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGtctc > 1:3355035/1‑70 (MQ=255) ccaGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGtctc > 1:143179/1‑70 (MQ=255) ccaGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGtctc > 1:2882744/1‑70 (MQ=255) ccaGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGtctc > 1:3107084/1‑70 (MQ=255) aGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGac < 1:4097581/71‑1 (MQ=255) aGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGac < 1:927473/71‑1 (MQ=255) aGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGa > 1:1449170/1‑70 (MQ=255) aGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGa > 1:393440/1‑70 (MQ=255) gATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGacaa < 1:2685271/71‑1 (MQ=255) tAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGacaaca > 1:271580/1‑71 (MQ=255) tAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGacaaca > 1:2753794/1‑71 (MQ=255) tAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGacaaca > 1:620415/1‑71 (MQ=255) tAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCGTCTCGacaaca < 1:950102/71‑1 (MQ=255) tAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCGTCTCGacaaca < 1:912387/71‑1 (MQ=255) aCCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTg < 1:1832983/71‑1 (MQ=255) gcgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGa > 1:2029742/1‑53 (MQ=255) cgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGat > 1:3053081/1‑71 (MQ=255) cgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGa > 1:1930019/1‑70 (MQ=255) cgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGa > 1:330724/1‑70 (MQ=255) cgatgGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGa < 1:1784748/70‑1 (MQ=255) cgatgGAAGAGATCAAGAAGATCTTTAAACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGat > 1:1825074/1‑71 (MQ=255) atgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTg < 1:2831696/62‑1 (MQ=255) gAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCt < 1:3505136/71‑1 (MQ=255) gAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCt < 1:602758/71‑1 (MQ=255) gAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCt < 1:3119735/71‑1 (MQ=255) gAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCt < 1:1042302/71‑1 (MQ=255) gAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCt < 1:1739661/71‑1 (MQ=255) agagATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACAtt > 1:3763745/1‑52 (MQ=255) gagaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACAtt < 1:2156931/51‑1 (MQ=255) gagaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTc < 1:1525410/71‑1 (MQ=255) gagaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTc < 1:2712426/71‑1 (MQ=255) agaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGtctc < 1:3012659/41‑1 (MQ=255) agaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGtctc < 1:156238/41‑1 (MQ=255) agaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGtctc < 1:1952072/41‑1 (MQ=255) agaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGtctc < 1:3621918/41‑1 (MQ=255) aTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGAGAACATTATCTGGTTTGatc < 1:1032987/61‑1 (MQ=255) aTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatc < 1:985728/61‑1 (MQ=255) aTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatc < 1:4090827/61‑1 (MQ=255) aTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAAc > 1:1922511/1‑71 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAAc > 1:1956348/1‑69 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAAc > 1:2231199/1‑69 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAAc > 1:3835095/1‑69 (MQ=255) gaagaTCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatcatc > 1:680341/1‑59 (MQ=255) gaagaTCTTTACACCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatca < 1:4092782/57‑1 (MQ=255) gaagaTCTTTACACCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatca < 1:3083830/57‑1 (MQ=255) gaagaTCTTTACACCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatca < 1:1797244/57‑1 (MQ=255) gaTCTTTACACCGGAATTCCGTAACCGTCTCGacaaca > 1:2558559/1‑38 (MQ=255) gaTCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatca < 1:792510/54‑1 (MQ=255) aTCTTTACACCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatca < 1:1857271/53‑1 (MQ=255) ttACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCa > 1:937898/1‑70 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATcc < 1:358586/68‑1 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATcc < 1:2984058/68‑1 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATcc < 1:2808284/68‑1 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATc < 1:3691525/67‑1 (MQ=255) acacCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATccacca > 1:1138513/1‑71 (MQ=255) acacCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATc < 1:2419083/70‑1 (MQ=255) acacCAGAATTCCGTAACCGGCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATc < 1:1839295/70‑1 (MQ=255) cacCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCa < 1:1281126/70‑1 (MQ=255) cacCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCa < 1:2286868/70‑1 (MQ=255) cacCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCa < 1:3203805/70‑1 (MQ=255) cacCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCa < 1:3262317/70‑1 (MQ=255) cacCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCa < 1:1258815/70‑1 (MQ=255) acCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAgg < 1:2739546/71‑1 (MQ=255) ccGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:958964/71‑1 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggttg < 1:1076096/71‑3 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggtgg < 1:680391/71‑1 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggtgg > 1:2419495/1‑71 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggtgg > 1:987609/1‑71 (MQ=255) | TCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGG > minE/581808‑581946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |