| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 597,552 | C→T | 54.2% | intergenic (+133/‑106) | serS → / → dmsA | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine/dimethyl sulfoxide reductase, anaerobic, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 597,552 | 0 | C | T | 54.2% | 19.0 / 156.1 | 107 | intergenic (+133/‑106) | serS/dmsA | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine/dimethyl sulfoxide reductase, anaerobic, subunit A |
| Reads supporting (aligned to +/- strand): ref base C (28/21); new base T (32/26); total (60/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
TTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTT > minE/597491‑597616 | ttACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTc > 1:1301658/1‑71 (MQ=255) cTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTaa < 1:3967523/54‑1 (MQ=255) cTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTaa < 1:615085/54‑1 (MQ=255) cTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTaa < 1:1192724/54‑1 (MQ=255) cTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTaa < 1:3440152/54‑1 (MQ=255) cTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATAc > 1:3828058/1‑70 (MQ=255) cTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATAc > 1:1576603/1‑70 (MQ=255) ttACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGAt < 1:1051757/63‑1 (MQ=255) ttACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGAt < 1:3094063/63‑1 (MQ=255) ttACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGAt < 1:486591/63‑1 (MQ=255) tACACGTAATACTACTTTCGAGTGAAAATCTCCCTATCTCTTTGATTTTCAAATTATTCg > 1:3705342/1‑60 (MQ=255) tACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAat > 1:341287/1‑54 (MQ=255) tACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAat > 1:3774044/1‑54 (MQ=255) tACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAat > 1:181407/1‑54 (MQ=255) cacGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGcc < 1:3259952/71‑1 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCt > 1:1718881/1‑70 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCt > 1:1713950/1‑70 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCt > 1:671155/1‑70 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta < 1:3648576/71‑1 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCt < 1:1012861/70‑1 (MQ=255) gTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCta < 1:3368462/70‑1 (MQ=255) tAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:3931804/1‑71 (MQ=255) tAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata < 1:1804619/71‑1 (MQ=255) tAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:1430003/1‑71 (MQ=255) tAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata < 1:778296/71‑1 (MQ=255) tAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:2432246/1‑71 (MQ=255) aTACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAatta > 1:3013140/1‑48 (MQ=255) tactacTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAg > 1:3105364/1‑71 (MQ=255) tactacTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAg > 1:1154346/1‑71 (MQ=255) tactacTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAg > 1:3002513/1‑71 (MQ=255) actactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGc > 1:1676684/1‑71 (MQ=255) actactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGc > 1:59412/1‑71 (MQ=255) ctactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta > 1:559673/1‑54 (MQ=255) ctactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAg > 1:3137747/1‑69 (MQ=255) ctactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAg > 1:392472/1‑69 (MQ=255) ctactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAg > 1:360497/1‑69 (MQ=255) ctactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAg > 1:3705768/1‑69 (MQ=255) tactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTc < 1:1879489/47‑1 (MQ=255) ctTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAAc > 1:3703838/1‑71 (MQ=255) tttCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGAt < 1:2876836/47‑1 (MQ=255) ttCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTg > 1:772451/1‑71 (MQ=255) ttCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGAt > 1:332498/1‑46 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc > 1:3289988/1‑71 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc > 1:4070835/1‑71 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc > 1:1104793/1‑71 (MQ=255) gAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGta < 1:2002338/46‑1 (MQ=255) gAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCt < 1:1966942/70‑1 (MQ=255) gTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCta > 1:351611/1‑69 (MQ=255) gTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCta > 1:585232/1‑69 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCg < 1:2944945/60‑1 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCg < 1:951159/60‑1 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTCAAATTATTc < 1:3580625/37‑1 (MQ=255) gAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta < 1:563984/52‑1 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCta < 1:2932782/51‑1 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCta < 1:1184768/51‑1 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTCAAATTATTCGa > 1:398637/1‑37 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTCAAATTATTCGa > 1:2588645/1‑37 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCt < 1:3817534/50‑1 (MQ=255) aaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGt > 1:3581083/1‑39 (MQ=255) aaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:785034/1‑52 (MQ=255) aaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:2266653/1‑52 (MQ=255) aaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:710492/1‑52 (MQ=255) aaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtat > 1:914740/1‑50 (MQ=255) aaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaat < 1:1637024/71‑1 (MQ=255) ctCTTCCGATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCTAACTGc < 1:2635503/60‑1 (MQ=255) ctCTTCCGATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc < 1:3973265/60‑1 (MQ=255) tCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataa > 1:1629118/1‑71 (MQ=255) tCTACCTATCTCTTTGATTTTTAAATTATTAGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataa > 1:1856191/1‑71 (MQ=255) tCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataa > 1:2898008/1‑71 (MQ=255) ccTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa > 1:1088358/1‑52 (MQ=255) ccTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa > 1:3428102/1‑52 (MQ=255) tctcTTTGATTTTCAAATTATTCGATGTATACAAGCCta < 1:3036661/39‑1 (MQ=255) tctcTTTGATTTTCAAATTATTCGATGTATACAAGCCta < 1:1500700/39‑1 (MQ=255) tctTTGATTTTTAAATTATTCGATTTATACAAGCCTATATAGCGAACTGCTATAGAaataa > 1:2584266/1‑61 (MQ=255) tctTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataa > 1:408644/1‑61 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:1136856/1‑39 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaaa > 1:4256063/1‑58 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaaa > 1:1455125/1‑58 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaaa > 1:2855699/1‑58 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaaa > 1:2761373/1‑58 (MQ=255) ctTTGATTTTTAAATTATTCGATGTATACAAGCCta < 1:739921/36‑1 (MQ=255) ctTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCg < 1:1034942/43‑1 (MQ=255) ctTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCg < 1:1865881/43‑1 (MQ=255) ctTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCg < 1:2914658/43‑1 (MQ=255) ctTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCg < 1:1863050/43‑1 (MQ=255) ctTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATAc > 1:26948/1‑71 (MQ=255) tttGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACg > 1:1674747/1‑71 (MQ=255) tttGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg < 1:2050442/42‑1 (MQ=255) tttGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg < 1:3441759/42‑1 (MQ=255) tttGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg < 1:2006073/42‑1 (MQ=255) ttGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGaa > 1:2075263/1‑43 (MQ=255) ttGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGaa > 1:1389285/1‑43 (MQ=255) ttGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGaa > 1:348987/1‑43 (MQ=255) ttGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGaa > 1:2490297/1‑43 (MQ=255) ttGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACgg > 1:1286606/1‑71 (MQ=255) tGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACgg < 1:2105002/70‑1 (MQ=255) gATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGtt > 1:2429868/1‑71 (MQ=255) gATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGt < 1:2609394/70‑1 (MQ=255) aTTTTTAAATTATTCGATGTATACAAGCCTATATAGCg < 1:352561/38‑1 (MQ=255) aTTTTTAAATTATTCGATGTATACAAGCCTATATAGCg < 1:1666385/38‑1 (MQ=255) | TTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTT > minE/597491‑597616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |