| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 623,375 | C→T | 67.6% | T19I (ACC→ATC) | msbA → | fused lipid transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 623,375 | 0 | C | T | 67.6% | 73.7 / 55.9 | 63 | T19I (ACC→ATC) | msbA | fused lipid transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (14/6); new base T (30/12); total (45/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGC > minE/623305‑623439 | aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:1076211/1‑71 (MQ=255)aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:1234952/1‑71 (MQ=255)aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:4187498/1‑71 (MQ=255)aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:1312927/1‑71 (MQ=255)aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc < 1:1799587/71‑1 (MQ=255)aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:893468/1‑71 (MQ=255) tGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAcc < 1:1687833/71‑1 (MQ=255) cTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCAt > 1:29049/1‑71 (MQ=255) cTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCAt > 1:2842070/1‑71 (MQ=255) cTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCAt > 1:2216433/1‑71 (MQ=255) tttGAATGGATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:2303158/1‑71 (MQ=255) tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:1578915/1‑71 (MQ=255) tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:700961/1‑71 (MQ=255) tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:564524/1‑71 (MQ=255) tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:346974/1‑71 (MQ=255) tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:3658607/1‑71 (MQ=255) ttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCt < 1:2527960/70‑1 (MQ=255) cATAACGACAAAGATCTCTCTACGTGGGAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:3701355/1‑71 (MQ=255) cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:3009296/1‑70 (MQ=255) cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:3587444/1‑71 (MQ=255) cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:3906961/1‑71 (MQ=255) cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc < 1:2674779/71‑1 (MQ=255) cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:808810/1‑71 (MQ=255) aaTAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:1257852/2‑71 (MQ=255) tAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCa < 1:1615868/53‑1 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:3084136/1‑49 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:3078786/1‑49 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTgcgc > 1:2676048/1‑57 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTg > 1:3221269/1‑54 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTg > 1:3640869/1‑54 (MQ=255) gACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGc > 1:4193730/1‑65 (MQ=255) gACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCt > 1:1719779/1‑71 (MQ=255) gACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCATTTCAAAGCGGGTCt > 1:3990834/1‑71 (MQ=255) aaGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATc > 1:98805/1‑71 (MQ=255) aaGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATc > 1:4074037/1‑71 (MQ=255) aaGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATc > 1:3458174/1‑71 (MQ=255) gATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGt < 1:2464907/71‑1 (MQ=255) ctACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTggc < 1:954459/67‑1 (MQ=255) tACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTc > 1:4088482/1‑46 (MQ=255) tACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggcg > 1:2860147/1‑71 (MQ=255) tACGTGGCAGACATTCCGCCGACTGTGGACAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggcg > 1:316824/1‑71 (MQ=255) gTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg < 1:3479223/71‑1 (MQ=255) gTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg < 1:1159562/71‑1 (MQ=255) gTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg > 1:4130883/1‑71 (MQ=255) cAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGtt < 1:1613261/71‑1 (MQ=255) aGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGtt < 1:2406333/70‑1 (MQ=255) aGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTa < 1:3965867/71‑1 (MQ=255) aGACATTCCGCCGACTGTGGCCAAACATTGCGCCTTTCAAAGCGGGTCTGATCGTgg > 1:1737833/1‑57 (MQ=255) aCATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:3884939/1‑71 (MQ=255) aCATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1517420/1‑71 (MQ=255) aCATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1846560/1‑71 (MQ=255) aCATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1847693/1‑71 (MQ=255) aCATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:785582/1‑71 (MQ=255) cATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATc < 1:297253/71‑1 (MQ=255) aTTCCGGCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATcc > 1:540772/1‑71 (MQ=255) aTTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATGGTGGCGGGCGTAGCGTTAATcc > 1:2159995/1‑71 (MQ=255) aTTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATcc > 1:3010453/1‑71 (MQ=255) aTTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1688822/1‑69 (MQ=255) aTTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1693549/1‑69 (MQ=255) tCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:1677659/1‑36 (MQ=255) tCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:3263746/1‑36 (MQ=255) ccgccgACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:2061519/1‑35 (MQ=255) ccgccgACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:3741719/1‑35 (MQ=255) cgccgACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCa < 1:3518362/70‑1 (MQ=255) cgccgACTGTGGCCAATCATTGCGCCTTTCAAAGCGAGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCaa < 1:197374/71‑1 (MQ=255) gccgACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATACTCaa < 1:4197124/70‑1 (MQ=255) cgACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGc < 1:4221783/71‑1 (MQ=255) cgACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGc < 1:2152337/71‑1 (MQ=255) tgtgGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagc > 1:1628822/1‑70 (MQ=255) tgtgGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagc > 1:3399556/1‑70 (MQ=255) tggcAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagccagc < 1:3842937/68‑1 (MQ=255) gCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagccagc < 1:2939662/70‑1 (MQ=255) | ATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGC > minE/623305‑623439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |