| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 656,044 | T→C | 18.4% | F44L (TTC→CTC) | ycbS → | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 656,044 | 0 | T | C | 18.4% | 172.8 / 34.5 | 76 | F44L (TTC→CTC) | ycbS | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base T (27/35); new base C (7/7); total (34/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
GGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGCCAGG > minE/655979‑656111 | ggCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaa < 1:2890803/70‑1 (MQ=255) gtggtgTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTTGATCCTGCTTTTTTCAAAga < 1:1049636/68‑1 (MQ=255) gtggtgTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAga < 1:3175248/68‑1 (MQ=255) gtggtgTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAga < 1:3803067/68‑1 (MQ=255) gtggtgTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACATGGTTCGATCCTGCTTTTTTCAAAga < 1:2977535/68‑1 (MQ=255) ttGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTtctc > 1:1487798/1‑56 (MQ=255) ttGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTCTCa > 1:3745713/1‑57 (MQ=255) ttGTGTCGGCAGCGTTCAATGAACAAGCTGAAACCTGGTTCGATCCTGCTTTTCTCa > 1:670521/1‑57 (MQ=39) tgtgTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCaa > 1:3595086/1‑71 (MQ=255) tgtgTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCaa > 1:3872112/1‑71 (MQ=255) gtgtCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCaa < 1:2609267/70‑1 (MQ=255) gCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaa < 1:3483707/50‑1 (MQ=255) gCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaa < 1:2193596/50‑1 (MQ=255) gCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaa < 1:1974703/50‑1 (MQ=255) cGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAt < 1:3363834/71‑1 (MQ=255) cGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAt < 1:3821941/71‑1 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTg > 1:3369707/1‑70 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTg > 1:277150/1‑70 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTg > 1:147340/1‑70 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTg > 1:1111867/1‑70 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGt > 1:3216162/1‑71 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGt > 1:3157311/1‑71 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGt > 1:532023/1‑71 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGt > 1:760928/1‑71 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGT‑‑CCGATTTGtc > 1:1393375/1‑70 (MQ=255) aaTGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGt < 1:3682366/70‑1 (MQ=255) aaTGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGt < 1:2395602/70‑1 (MQ=255) aaTGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGtc > 1:527349/1‑71 (MQ=255) gCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCg > 1:956737/1‑71 (MQ=255) gCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCg > 1:315458/1‑71 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGt > 1:542232/1‑71 (MQ=255) aaGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGcc < 1:1046363/56‑1 (MQ=255) aaGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGcc < 1:2890797/56‑1 (MQ=255) aaGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGcc < 1:2410567/56‑1 (MQ=255) aaGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGcc < 1:3736224/56‑1 (MQ=255) aGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAt < 1:1257035/48‑1 (MQ=255) aGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAt < 1:649418/48‑1 (MQ=255) aGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTc > 1:2362800/1‑70 (MQ=255) aGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTc > 1:1776685/1‑70 (MQ=255) cTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtgg > 1:3995252/1‑48 (MQ=255) cTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtgg > 1:3482826/1‑48 (MQ=255) aaCCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAg < 1:1412112/71‑1 (MQ=255) aaCCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAg < 1:2711978/71‑1 (MQ=255) aaCCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAg < 1:2643769/71‑1 (MQ=255) aaCCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAg < 1:244397/71‑1 (MQ=255) gTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaa < 1:3406802/71‑1 (MQ=255) gTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaa < 1:144427/71‑1 (MQ=255) gTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaa > 1:1037410/1‑70 (MQ=255) gTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaa > 1:2169629/1‑70 (MQ=255) tCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaaa > 1:1700560/1‑70 (MQ=255) gATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:1258585/71‑1 (MQ=255) gATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:3380203/71‑1 (MQ=255) gATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:3968915/71‑1 (MQ=255) gATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:1094825/71‑1 (MQ=255) gATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:650257/71‑1 (MQ=255) gATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:1444422/71‑1 (MQ=255) gATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:3993358/71‑1 (MQ=255) aTCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:3262356/70‑1 (MQ=255) aTCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:2372477/57‑1 (MQ=255) aTCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:2812611/57‑1 (MQ=255) aTCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:3252275/57‑1 (MQ=255) aTCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:1527848/57‑1 (MQ=255) tCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATaa > 1:3045215/1‑71 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:371054/54‑1 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:755216/54‑1 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:2787805/54‑1 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:1161496/54‑1 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:3837653/54‑1 (MQ=255) tGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGttt > 1:3496537/1‑48 (MQ=255) gCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGc > 1:1009676/1‑70 (MQ=255) gCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGc > 1:970320/1‑70 (MQ=255) gCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGc > 1:1292138/1‑70 (MQ=255) gCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGc > 1:1570606/1‑70 (MQ=255) gCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGc > 1:2345721/1‑70 (MQ=255) gCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGc > 1:2857762/1‑70 (MQ=255) gCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGc > 1:3019517/1‑70 (MQ=255) gCTTTTTACAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGc > 1:2839214/1‑70 (MQ=255) tttctCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGCCAgg > 1:1345417/1‑71 (MQ=255) tttctCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGCCAgg > 1:1550758/1‑71 (MQ=255) | GGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACGCCAGG > minE/655979‑656111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |