| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 694,025 | T→A | 7.7% | L193* (TTA→TAA) | hyaF → | protein involved in nickel incorporation into hydrogenase‑1 proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 694,025 | 0 | T | A | 7.7% | 181.2 / 7.0 | 65 | L193* (TTA→TAA) | hyaF | protein involved in nickel incorporation into hydrogenase‑1 proteins |
| Reads supporting (aligned to +/- strand): ref base T (35/25); new base A (3/2); total (38/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01 | |||||||||||
GCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTAC > minE/693960‑694094 | gaaTGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTAc > 1:1018324/3‑68 (MQ=255) tGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTa > 1:3603487/1‑64 (MQ=255) tGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCAt > 1:993049/1‑69 (MQ=255) tGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCAt > 1:4209001/1‑69 (MQ=255) tACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGc > 1:2406717/1‑68 (MQ=255) tACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGc > 1:3431197/1‑68 (MQ=255) tACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGc > 1:1819717/1‑68 (MQ=255) tACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGc > 1:182797/1‑68 (MQ=255) aCTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAgg > 1:1266944/1‑71 (MQ=255) aCTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAg < 1:2929394/70‑1 (MQ=255) aCTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAg < 1:485524/70‑1 (MQ=255) acaTGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATc > 1:758742/1‑71 (MQ=255) acaTGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATc > 1:3841775/1‑71 (MQ=255) acaTGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATc > 1:3778880/1‑71 (MQ=255) tGTACGTAACCCCGACGCGCATCCGCACAGCATTAATCTGACGCAATTa < 1:1404315/49‑1 (MQ=255) tGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTg < 1:2312783/65‑1 (MQ=255) tACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGAt > 1:3507128/1‑65 (MQ=255) tACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGAt > 1:432234/1‑65 (MQ=255) tACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGAt > 1:406104/1‑65 (MQ=255) tACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCtt > 1:3010933/1‑71 (MQ=255) tACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCtt > 1:1175800/1‑71 (MQ=255) cGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTg < 1:287444/61‑1 (MQ=255) cGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCt < 1:565606/68‑1 (MQ=255) cGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCt < 1:2542835/68‑1 (MQ=255) gTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGa < 1:4182692/55‑1 (MQ=255) gTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt < 1:2078415/71‑1 (MQ=255) gTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt < 1:114993/71‑1 (MQ=255) gTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt < 1:2611395/71‑1 (MQ=255) gTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCt < 1:970226/67‑1 (MQ=255) gTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGCCttttt < 1:163212/71‑1 (MQ=255) tAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttt > 1:2272770/1‑68 (MQ=255) tAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtc < 1:3993553/71‑1 (MQ=255) tAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtc < 1:2039706/71‑1 (MQ=255) tAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtc < 1:1221620/71‑1 (MQ=255) tAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATAACCCATCAGCGAGGCTGATCGGCTTTTtc < 1:3948967/71‑1 (MQ=255) tAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATAACCCATCAGCGAGGCTGATCGGCTTTTtc < 1:3821746/71‑1 (MQ=255) ccccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct < 1:3672447/71‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa > 1:1562969/1‑69 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa > 1:3204616/1‑69 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa > 1:1546028/1‑69 (MQ=255) cgcAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGtctc < 1:2256891/71‑1 (MQ=255) caGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGtctc < 1:1970266/61‑1 (MQ=255) aTTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct < 1:2481856/49‑1 (MQ=255) aTTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtgt < 1:1127775/60‑1 (MQ=255) aTTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtgt < 1:3552341/60‑1 (MQ=255) ttAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtgt < 1:3675541/59‑1 (MQ=255) aaTCTGACGCAATTACCCATCAGCGAGGCTGATCGGCtttt > 1:1153475/1‑41 (MQ=255) aaTCTGACGCAATTACCCATCAGCGAGGCTGATCGGCtttt > 1:1755178/1‑41 (MQ=255) aaTCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAttat > 1:2626963/1‑71 (MQ=255) aaTCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAatat > 1:3846800/1‑71 (MQ=255) aaTCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAatat > 1:940315/1‑71 (MQ=255) tGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:3043602/1‑38 (MQ=255) tGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:1532564/1‑38 (MQ=255) tGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATAtt > 1:516745/1‑68 (MQ=255) tGACGCAATAACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATAtt > 1:1516013/1‑68 (MQ=255) tGACGCAATAACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATAtt > 1:2712543/1‑68 (MQ=255) tGACGCAATAACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATAtt > 1:2288556/1‑68 (MQ=255) gACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATAtt > 1:1622142/1‑67 (MQ=255) gCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTc > 1:3155778/1‑71 (MQ=255) gCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTc > 1:726336/1‑71 (MQ=255) gCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTc > 1:2938781/1‑71 (MQ=255) gCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTc > 1:608579/1‑71 (MQ=255) gCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTc > 1:1135076/1‑71 (MQ=255) cAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCg < 1:1017662/71‑1 (MQ=255) cAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCg < 1:292288/71‑1 (MQ=255) ttACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTAc < 1:3725214/71‑1 (MQ=255) ttACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTAc > 1:2636081/1‑71 (MQ=255) tACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTa > 1:2845051/1‑69 (MQ=255) tACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTAc > 1:538600/1‑70 (MQ=255) tACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTAc > 1:1845386/1‑70 (MQ=255) tACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTAc > 1:1419695/1‑70 (MQ=255) | GCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTAC > minE/693960‑694094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |