| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 158,183 | A→G | 75.8% | N41S (AAC→AGC) | dgt → | deoxyguanosine triphosphate triphosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 158,183 | 0 | A | G | 75.8% | 120.1 / 36.8 | 62 | N41S (AAC→AGC) | dgt | deoxyguanosine triphosphate triphosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (7/8); new base G (27/20); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGGGCGTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGC > minE/158118‑158251 | gCAGGGCGTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGctct > 1:838170/1‑70 (MQ=255)gCAGGGCGTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGctct > 1:409481/1‑70 (MQ=255)gCAGGGCGTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGAGATCGCGGGCGTATCATCAGctct > 1:1774046/1‑70 (MQ=255) gTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAAt < 1:552569/71‑1 (MQ=255) gTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAAt < 1:495277/71‑1 (MQ=255) gTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAAt < 1:2558184/71‑1 (MQ=255) ccGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCaa > 1:1899319/1‑63 (MQ=255) ccGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCa > 1:2600426/1‑62 (MQ=255) gAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATtcgt < 1:1545908/66‑1 (MQ=255) gAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATtcgt < 1:1644034/66‑1 (MQ=255) aaCATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTg < 1:3566001/71‑1 (MQ=255) aaCATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTg < 1:3380443/71‑1 (MQ=255) tGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGcaa > 1:671470/1‑70 (MQ=255) agaTCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGcaacaa < 1:932396/71‑1 (MQ=255) agaTCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGcaaca < 1:3178224/70‑1 (MQ=255) agaTCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGcaaca < 1:3427724/70‑1 (MQ=255) agaTCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGcaacaa < 1:507292/71‑1 (MQ=255) aTCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACaaaa < 1:1449693/71‑1 (MQ=255) aTCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACaaaa < 1:2999894/71‑1 (MQ=255) aTCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACaaaa < 1:2534584/71‑1 (MQ=255) cTGCGGATCTTCGAGAGCGATCGCGTGCGTATCATCAGCTCTCCTGCAATTCGTCGTCTGCAACAAAAGAc > 1:802025/1‑71 (MQ=255) cTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTcgtcgt < 1:3336338/57‑1 (MQ=255) cTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTcgtcgt < 1:1462751/57‑1 (MQ=255) cTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTcgtcgt < 1:3927918/57‑1 (MQ=255) cTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGa > 1:3646674/1‑70 (MQ=255) cTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGAc > 1:4197889/1‑71 (MQ=255) cTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGAc > 1:288026/1‑71 (MQ=255) cTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGAc > 1:2132722/1‑71 (MQ=255) cTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATAAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGAc > 1:2742967/1‑71 (MQ=255) tGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGAcc > 1:427697/1‑71 (MQ=255) tGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGAcc > 1:2185059/1‑71 (MQ=255) cGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGtttt < 1:3671814/69‑1 (MQ=255) cGAGAGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGtttt < 1:1325572/69‑1 (MQ=255) cGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGttt < 1:4020885/68‑1 (MQ=255) gagaGCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGtttt < 1:4197196/68‑1 (MQ=255) gagaGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTc < 1:1200265/70‑1 (MQ=255) gagCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCAc > 1:1590121/1‑71 (MQ=255) gagCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCAc > 1:1350903/1‑71 (MQ=255) agCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGAc > 1:1720485/1‑56 (MQ=255) agCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGAc > 1:4178368/1‑56 (MQ=255) agCGATCGCGGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGAc > 1:2777435/1‑56 (MQ=255) cgcgGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCa > 1:1207871/1‑63 (MQ=255) cgcgGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCa > 1:1230690/1‑63 (MQ=255) cgcgGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCa > 1:1622970/1‑63 (MQ=255) cgcgGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCa > 1:2964535/1‑63 (MQ=255) gcgGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACaaaa > 1:1879181/1‑46 (MQ=255) gcgGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACaaa > 1:417801/1‑45 (MQ=255) gcgGGCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAgcgc < 1:3488188/71‑1 (MQ=255) gcgGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAgcg > 1:2549638/1‑70 (MQ=255) gggCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCaa < 1:3979954/71‑1 (MQ=255) gggCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCa < 1:3897997/70‑1 (MQ=255) ggCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAAt < 1:3743393/71‑1 (MQ=255) ggCGTATCATCAGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAAt < 1:1203037/71‑1 (MQ=255) tATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTc < 1:3957245/53‑1 (MQ=255) atcatcAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATgccgc > 1:398898/1‑71 (MQ=255) tcatcaGCTCTCCGGCAATTCGTCGTCTGCAAAAAAAGACACAGGttt > 1:1045964/1‑48 (MQ=38) atcaGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCAc > 1:1462151/1‑52 (MQ=255) atcaGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCAc > 1:4210303/1‑52 (MQ=255) atcaGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCAc > 1:3181922/1‑52 (MQ=255) atcaGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCAc > 1:897566/1‑52 (MQ=255) atcaGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCAc > 1:3186892/1‑52 (MQ=255) atcaGCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTg > 1:1959697/1‑54 (MQ=255) aaCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGttttt > 1:2807009/1‑45 (MQ=255) aaCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTc > 1:2350676/1‑46 (MQ=255) aaCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTc > 1:4198639/1‑46 (MQ=255) aaCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTc > 1:2843960/1‑46 (MQ=255) aaCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTc > 1:3822887/1‑46 (MQ=255) aaCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTgc < 1:3080277/70‑1 (MQ=255) | GCAGGGCGTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGC > minE/158118‑158251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |