| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 813,910 | A→G | 44.6% | C60C (TGT→TGC) | prsA ← | phosphoribosylpyrophosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 813,910 | 0 | A | G | 44.6% | 29.1 / 131.9 | 96 | C60C (TGT→TGC) | prsA | phosphoribosylpyrophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (23/30); new base G (22/20); total (46/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCG > minE/813845‑813976 | gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGt > 1:86423/1‑71 (MQ=255)gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGt > 1:1544091/1‑71 (MQ=255) cGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTg < 1:1780505/71‑1 (MQ=255) gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg > 1:417040/1‑67 (MQ=255) cgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGgat > 1:406555/1‑71 (MQ=255) cgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGgat > 1:2527988/1‑71 (MQ=255) cATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAata < 1:2004841/71‑1 (MQ=255) cATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAata < 1:119781/71‑1 (MQ=255) cATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAata < 1:2272481/71‑1 (MQ=255) aTCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAatat > 1:2936586/1‑71 (MQ=255) aTCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAatat > 1:3777274/1‑71 (MQ=255) aTCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGAACAAGTGGACTGGATGATGAAAata > 1:2616961/1‑70 (MQ=255) tCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGcaca < 1:4220634/47‑1 (MQ=255) tCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:3156896/68‑1 (MQ=255) tCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:2595125/68‑1 (MQ=255) tCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:468838/68‑1 (MQ=255) tCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:3416829/68‑1 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAatat > 1:1564033/1‑68 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAatat > 1:393256/1‑68 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAatat > 1:3976732/1‑68 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcac > 1:402864/1‑71 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcac < 1:399061/71‑1 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATcac < 1:1124986/71‑1 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATcac < 1:3968658/71‑1 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATcac < 1:1757355/71‑1 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATcac < 1:4089036/71‑1 (MQ=255) gccATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGa > 1:3868085/2‑50 (MQ=255) aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTATGGGCACAAGTGGa > 1:2104473/1‑50 (MQ=255) aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcac < 1:2941083/70‑1 (MQ=255) aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGa > 1:2354567/1‑50 (MQ=255) aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGa > 1:410215/1‑50 (MQ=255) aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGa > 1:1327469/1‑50 (MQ=255) aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATcacc < 1:1076530/71‑1 (MQ=255) ccATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAg < 1:733618/45‑1 (MQ=255) ccATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAg < 1:1367325/45‑1 (MQ=255) aTAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGgatgat < 1:3974164/56‑1 (MQ=255) aTAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGgatgat < 1:2749691/56‑1 (MQ=255) aTAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGgatgat < 1:1312225/56‑1 (MQ=255) tAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcacc < 1:3717022/67‑1 (MQ=255) tAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcacc < 1:4118794/67‑1 (MQ=255) tAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcacc < 1:3779264/67‑1 (MQ=255) tAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcacc < 1:3814907/67‑1 (MQ=255) tAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTg < 1:3320635/44‑1 (MQ=255) tAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTg < 1:3039227/44‑1 (MQ=255) aaCTACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACaact > 1:2589001/1‑40 (MQ=38) aaCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACcgc > 1:4173636/1‑71 (MQ=255) aaCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACcgc > 1:4053965/1‑71 (MQ=255) aaCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACcgc > 1:1317147/1‑71 (MQ=255) aCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACcgcg > 1:2045954/1‑71 (MQ=255) aCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACcgcg > 1:1466184/1‑71 (MQ=255) aCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACcgcg > 1:2998924/1‑71 (MQ=255) cGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAat < 1:495557/58‑1 (MQ=255) cGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAat < 1:2849373/58‑1 (MQ=255) cGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAat < 1:2509283/58‑1 (MQ=255) gACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACTAGTGGACTGGATGATGAAAATATCACCACCGCGTa < 1:511134/71‑1 (MQ=255) aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTAc < 1:3475351/71‑1 (MQ=255) aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTAc < 1:1909907/71‑1 (MQ=255) aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTAc < 1:814788/71‑1 (MQ=255) cTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTAc < 1:3480359/70‑1 (MQ=255) aTTCCATCAGGTTGTCGTTAGTAGGGGCTCAAGTGGACTGGATGATGAAAATATcac > 1:3582207/1‑57 (MQ=255) aTTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcac > 1:1841352/1‑57 (MQ=255) aTTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcac > 1:3519183/1‑57 (MQ=255) aTTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcac > 1:4170487/1‑57 (MQ=255) aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAatat > 1:3585028/1‑54 (MQ=255) aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAatat > 1:3358509/1‑54 (MQ=255) ttCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACAtttt < 1:1190188/71‑1 (MQ=255) ccATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTc > 1:619970/1‑70 (MQ=255) ccATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCa > 1:1695248/1‑71 (MQ=255) ccATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCa > 1:933634/1‑71 (MQ=255) ccATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCa > 1:2382057/1‑71 (MQ=255) aTCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCAtt < 1:2190274/71‑1 (MQ=255) aTCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCAtt < 1:763115/71‑1 (MQ=255) tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTa < 1:1254562/71‑1 (MQ=255) cAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTaa > 1:2257584/1‑71 (MQ=255) cAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTaa > 1:1475816/1‑71 (MQ=255) cAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTaa > 1:3783108/1‑71 (MQ=255) gTTGTCGTTAGTAGGTGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCAtt < 1:3345220/66‑1 (MQ=255) gTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCAtt < 1:580893/66‑1 (MQ=255) gTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAAtt < 1:3048996/70‑1 (MQ=255) ttGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGaa < 1:3020795/38‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATca > 1:2338917/1‑44 (MQ=255) tGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTa > 1:1434028/1‑54 (MQ=255) tGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTa > 1:3198212/1‑54 (MQ=255) gTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGaaaa > 1:650343/1‑38 (MQ=255) gTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGaaaa > 1:91978/1‑38 (MQ=255) gTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGc > 1:3429919/1‑71 (MQ=255) gTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCAtt < 1:2925083/60‑1 (MQ=255) ttAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAAt < 1:1708762/62‑1 (MQ=255) ttAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAAt < 1:4218416/62‑1 (MQ=255) tagtatGGGCGCAAGTTGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAAt < 1:1555202/61‑1 (MQ=255) ggggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACtt < 1:476097/70‑1 (MQ=255) ggggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACtt < 1:579497/70‑1 (MQ=255) gggCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAAt < 1:2349778/55‑1 (MQ=255) gggCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAAt < 1:932136/55‑1 (MQ=255) ggCACAAGTGGCCTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTc > 1:2516398/1‑69 (MQ=255) ggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTc > 1:3340508/1‑69 (MQ=255) ggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCg > 1:3779225/1‑70 (MQ=255) | GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCG > minE/813845‑813976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |