| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 880,753 | G→A | 66.0% | intergenic (+72/‑122) | yciM → / → pyrF | conserved hypothetical protein/orotidine‑5'‑phosphate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 880,753 | 0 | G | A | 66.0% | 51.7 / 47.5 | 50 | intergenic (+72/‑122) | yciM/pyrF | conserved hypothetical protein/orotidine‑5'‑phosphate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base G (4/13); new base A (10/23); total (14/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
AATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCT > minE/880693‑880819 | aaTCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGaaaaa < 1:2018017/71‑1 (MQ=255) tCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGc < 1:1658539/71‑1 (MQ=255) tttAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGaaaaa < 1:2526127/63‑1 (MQ=255) tttAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGc > 1:4061281/1‑69 (MQ=255) gTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAggg < 1:3638266/70‑1 (MQ=255) caacaTACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTg < 1:1353119/60‑1 (MQ=255) caacaTACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGaa < 1:3662649/71‑1 (MQ=255) caTACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAAt > 1:1435265/1‑70 (MQ=255) aTACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCg < 1:2001735/71‑1 (MQ=255) tACTAATTATTAATGTTCCATTGTGCTTCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGc < 1:628437/71‑1 (MQ=255) tACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGc < 1:1286730/71‑1 (MQ=255) cTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCa < 1:1693031/70‑1 (MQ=255) aaTTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGc < 1:737123/52‑1 (MQ=255) aaTTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGc < 1:2940022/52‑1 (MQ=255) aaTTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGc < 1:3780275/52‑1 (MQ=255) aaTTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGc < 1:2019831/52‑1 (MQ=255) aaTTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGc < 1:601841/52‑1 (MQ=255) aaTTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGc < 1:2727078/52‑1 (MQ=255) attattAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAggg < 1:1151154/56‑1 (MQ=255) attaATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGgaga < 1:1145647/57‑1 (MQ=255) attaATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGc < 1:4103888/63‑1 (MQ=255) attaATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGtt > 1:3765661/1‑70 (MQ=255) ttaATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGc < 1:814687/62‑1 (MQ=255) aTGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:383897/71‑1 (MQ=255) ttCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttttt < 1:2371494/70‑1 (MQ=255) ttCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttttt < 1:505225/70‑1 (MQ=255) ttCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttttt > 1:17839/1‑70 (MQ=255) ttCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttttt < 1:2928400/70‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTa < 1:2505342/70‑1 (MQ=255) cATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTAtt > 1:3221164/1‑71 (MQ=255) ttGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGgag < 1:1447155/44‑1 (MQ=255) ttGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGgag < 1:3073212/44‑1 (MQ=255) tgtgCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTAttt > 1:291834/1‑69 (MQ=255) tgtgCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTc > 1:873255/1‑70 (MQ=255) tgtgCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTc > 1:3199666/1‑70 (MQ=255) gtgCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTc > 1:1302132/1‑69 (MQ=255) tgCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCAc < 1:1040397/71‑1 (MQ=255) ccGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACggg > 1:2849932/1‑70 (MQ=255) cGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACggg > 1:465657/1‑69 (MQ=255) cGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACg < 1:2663887/67‑1 (MQ=255) cGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACg < 1:2221353/67‑1 (MQ=255) cGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTa < 1:3949606/71‑1 (MQ=255) cGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTa < 1:2284736/71‑1 (MQ=255) gCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACggg > 1:3632574/1‑67 (MQ=255) gCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAg > 1:2712670/1‑70 (MQ=255) aaCGCCGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAAt > 1:2196243/1‑71 (MQ=255) aaCGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAAt < 1:3049270/71‑1 (MQ=255) aaCGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAAt < 1:2100411/71‑1 (MQ=255) gacgGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCt < 1:3801442/71‑1 (MQ=255) gacgGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCt < 1:1237018/71‑1 (MQ=255) | AATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCT > minE/880693‑880819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |