| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 163,666 | C→T | 42.4% | E77K (GAA→AAA) | yaeI ← | predicted phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 163,666 | 0 | C | T | 42.4% | 32.2 / 72.9 | 59 | E77K (GAA→AAA) | yaeI | predicted phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (19/15); new base T (9/16); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
GCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGC > minE/163596‑163732 | gCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTc < 1:2729485/71‑1 (MQ=255)gCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTc < 1:2046455/71‑1 (MQ=255) aaaTTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATg > 1:877810/1‑69 (MQ=255) aaaTTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGc > 1:3781445/1‑70 (MQ=255) atatCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGc < 1:1093407/65‑1 (MQ=255) atatCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCa < 1:2079240/66‑1 (MQ=255) tCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGt > 1:1657799/1‑70 (MQ=255) tCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGt > 1:2845038/1‑70 (MQ=255) tCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGt > 1:1801247/1‑70 (MQ=255) aaaTAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCt > 1:2721165/1‑49 (MQ=255) aaaTAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCt > 1:2449704/1‑49 (MQ=255) aaaTAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCt > 1:2340727/1‑49 (MQ=255) aaaTAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCt > 1:1601359/1‑49 (MQ=255) aaaTAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCt > 1:4237282/1‑49 (MQ=255) aaaTAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCt > 1:523374/1‑49 (MQ=255) ataataCGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTc > 1:1759603/1‑46 (MQ=255) ataataCGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTc > 1:3051838/1‑46 (MQ=255) ataataCGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTc > 1:3047118/1‑46 (MQ=255) ataataCGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGa > 1:3527563/1‑70 (MQ=255) ataCGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATc < 1:3231427/71‑1 (MQ=255) taCGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCaa < 1:2800449/50‑1 (MQ=255) aCGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAAt < 1:1799712/68‑1 (MQ=255) tCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaa < 1:3195542/71‑1 (MQ=255) tCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaa < 1:2460132/71‑1 (MQ=255) tCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaa < 1:901655/71‑1 (MQ=255) tAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAAc < 1:3961737/71‑1 (MQ=255) tAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAAc < 1:970834/71‑1 (MQ=255) atatCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAAc > 1:4253260/1‑69 (MQ=255) atatCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACga < 1:2894621/71‑1 (MQ=255) atatCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACga < 1:1406029/71‑1 (MQ=255) atatCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACga < 1:1437827/71‑1 (MQ=255) atatCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaaa > 1:3933596/1‑59 (MQ=255) atatCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAAc > 1:3561723/1‑69 (MQ=255) atatCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAAc > 1:254177/1‑69 (MQ=255) tatCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACaa < 1:4168523/65‑1 (MQ=255) atCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGc > 1:1324614/1‑52 (MQ=255) tCAAATCGTGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaa < 1:3699094/55‑1 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAAt < 1:1756047/46‑1 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAAt < 1:4009647/46‑1 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAAt < 1:332645/46‑1 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAAt > 1:3362512/1‑46 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAAt < 1:348578/46‑1 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAAt < 1:1494328/46‑1 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaaa > 1:3446083/1‑56 (MQ=255) tCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATcgcg > 1:4180364/1‑38 (MQ=255) tCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATcgcg > 1:3670435/1‑38 (MQ=255) tCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGc > 1:778781/1‑51 (MQ=255) tCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaa < 1:2619282/55‑1 (MQ=255) tCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaa < 1:1544940/55‑1 (MQ=255) tCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaa < 1:1604181/55‑1 (MQ=255) cAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGagag > 1:3876348/1‑70 (MQ=255) aaaTCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTa < 1:1438397/71‑1 (MQ=255) aaaTCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTa < 1:4217904/71‑1 (MQ=255) aaTCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACga < 1:3711240/65‑1 (MQ=255) aTCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAAt > 1:3563221/1‑71 (MQ=255) aTCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAAt > 1:2669898/1‑71 (MQ=255) gtgCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGga < 1:3255875/69‑1 (MQ=255) gggCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGga < 1:1912181/71‑1 (MQ=255) gggCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGga < 1:1345548/71‑1 (MQ=255) ggCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGgag < 1:3138406/71‑1 (MQ=255) tGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGc > 1:324021/1‑71 (MQ=255) tGTTTTATG‑CAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGc > 1:3035844/1‑70 (MQ=255) tGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGc > 1:2158575/1‑71 (MQ=255) ttCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAAt < 1:1361478/59‑1 (MQ=255) | GCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGC > minE/163596‑163732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |