| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,010,827 | C→T | 6.0% | Y85Y (TAC→TAT) | rstB → | sensory histidine kinase in two‑component regulatory system with RstA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,010,827 | 0 | C | T | 6.0% | 383.3 / 9.3 | 117 | Y85Y (TAC→TAT) | rstB | sensory histidine kinase in two‑component regulatory system with RstA |
| Reads supporting (aligned to +/- strand): ref base C (55/55); new base T (2/5); total (57/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 120 total aligned reads displayed.
GGGTAAAACTCTGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAGTACACGT > minE/1010758‑1010897 | gggTAAAACTCTGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATAc > 1:2729867/1‑70 (MQ=255) aaaCTCTGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt > 1:2032726/1‑71 (MQ=255) aaCTCTGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt > 1:3580212/1‑70 (MQ=255) ctctGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATc > 1:901395/1‑66 (MQ=255) ctctGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATc > 1:2696896/1‑66 (MQ=255) ctctGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTtgat > 1:3282860/1‑71 (MQ=255) ctctGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTtgat > 1:2736398/1‑71 (MQ=255) ctctGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTtgat > 1:3890886/1‑71 (MQ=255) ctGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTtgatga > 1:4095527/1‑71 (MQ=255) ctGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTtgatga > 1:3804111/1‑71 (MQ=255) tGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:1587459/65‑1 (MQ=255) tGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:1960692/65‑1 (MQ=255) tGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:3280574/65‑1 (MQ=255) tGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTtgatga > 1:2949320/1‑70 (MQ=255) aaGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTgatgat < 1:2604526/68‑1 (MQ=255) aaGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATAtt < 1:1244997/71‑1 (MQ=255) agagATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATAttt > 1:2144940/1‑71 (MQ=255) agagATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATAttt > 1:2688380/1‑71 (MQ=255) gagaTGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTc < 1:2309035/71‑1 (MQ=255) agaTGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATAttt > 1:2726941/1‑69 (MQ=255) aTGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:1522335/57‑1 (MQ=255) aTGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:399489/57‑1 (MQ=255) aTGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:3657591/57‑1 (MQ=255) aTGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:3528430/57‑1 (MQ=255) aTGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:1045858/57‑1 (MQ=255) aTGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCAt < 1:4230779/71‑1 (MQ=255) gATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCa > 1:4039593/1‑71 (MQ=255) gATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCa > 1:2173630/1‑71 (MQ=255) gATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCa > 1:3437959/1‑71 (MQ=255) gATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCa < 1:1897110/71‑1 (MQ=255) gATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCa < 1:426754/71‑1 (MQ=255) tttAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:1905379/52‑1 (MQ=255) tttAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCa < 1:88789/69‑1 (MQ=255) tttAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCa < 1:2891462/69‑1 (MQ=255) tttAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCAcc < 1:916102/71‑1 (MQ=255) tttAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCAc > 1:165319/1‑70 (MQ=255) ttATATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATAttt < 1:1427359/61‑1 (MQ=255) ttAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATAttt < 1:3916122/61‑1 (MQ=255) ttAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATAttt < 1:2599944/61‑1 (MQ=255) ttAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCAcc > 1:1418454/1‑70 (MQ=255) tAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATTCCATCTTGATGATATTTCCATGCACCg < 1:3758567/70‑1 (MQ=255) ctctctTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCAcc > 1:1130767/1‑64 (MQ=255) ctctctTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCAcc > 1:3396374/1‑64 (MQ=255) ctctTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTg > 1:4118/1‑71 (MQ=255) ctctTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTg > 1:4133515/1‑71 (MQ=255) tctTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTgg > 1:1815905/1‑71 (MQ=255) ctTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTggc > 1:715824/1‑71 (MQ=255) tttCGATCTGCGTGTCGAGCCACTGAGTAAATATCATCAagatgg < 1:1277065/45‑2 (MQ=25) tttCGATCTGCGTGTCGAGCCACTGAGTAAATATCATCAagatgg < 1:1510157/45‑2 (MQ=25) tttCGATCTGCGTGTCGAGCCACTGAGTAAATATCATCAagatgg < 1:2101237/45‑2 (MQ=25) tttCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:583491/40‑1 (MQ=255) tttCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:3093674/40‑1 (MQ=255) tttCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:4181868/40‑1 (MQ=255) tttCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:3393719/40‑1 (MQ=255) tttCGATCTGCGTGTCGAGACACTGAGTAAATACCATCtt < 1:4193140/40‑1 (MQ=255) tttCGATCTCCGTGTCGAGCCACTGAGTAAATACCATCtt < 1:1630160/40‑1 (MQ=255) ttCGATCTGCGTGTCGAGCCACTGAGTAAATATCATCAagatgg < 1:988371/44‑2 (MQ=25) tCGGTCTGCGTGTCGAGCCACTGAGTAAATATCATCAAGATGgta > 1:2855237/1‑45 (MQ=18) tCGATCTGCGTGTCGAGCCACTGAGTAAATATCATCAAGATGgta > 1:2390632/1‑45 (MQ=21) tCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTtga > 1:201095/1‑40 (MQ=255) cGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTggcggc > 1:2425583/1‑70 (MQ=255) cTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATAttt > 1:2211669/1‑43 (MQ=255) cTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCa > 1:637345/1‑46 (MQ=255) cTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCa > 1:3721180/1‑46 (MQ=255) cTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCa > 1:1504285/1‑46 (MQ=255) cTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCa > 1:68821/1‑46 (MQ=255) cTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAAt > 1:1127982/1‑71 (MQ=255) cGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGa < 1:1852828/65‑1 (MQ=255) cGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAAtt < 1:1603708/69‑1 (MQ=255) cGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAAt < 1:1538791/68‑1 (MQ=255) gAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCt > 1:2973218/1‑71 (MQ=255) aGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTg < 1:3420125/71‑1 (MQ=255) aGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTg < 1:2187551/71‑1 (MQ=255) aGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTg < 1:3498856/71‑1 (MQ=255) aGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTg < 1:2316142/71‑1 (MQ=255) aGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTg < 1:4001468/71‑1 (MQ=255) aGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTg < 1:2066546/71‑1 (MQ=255) aGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTg < 1:3745728/71‑1 (MQ=255) gCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTg > 1:840732/1‑70 (MQ=255) gCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTg > 1:4104180/1‑70 (MQ=255) aCTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGAc > 1:2828165/1‑70 (MQ=255) cTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGa < 1:2899699/71‑1 (MQ=255) cTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGa < 1:2315262/71‑1 (MQ=255) gAGTAAATACCATCTTGATGATATTTCCATGCACCGa > 1:1512230/1‑37 (MQ=255) gAGTAAATACCATCTTGATGATATTTCCATGCACCGa > 1:2262486/1‑37 (MQ=255) gAGTAAATACCATCTTGATGATATTTCCATGCACCGa > 1:2195790/1‑37 (MQ=255) gAGTAAATACCATCTTGATGATATTTCCATGCACCGa > 1:3683958/1‑37 (MQ=255) gAGTAAATACCATCTTGATGATATTTCCATGCACCGa > 1:3398054/1‑37 (MQ=255) aGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGAt > 1:1734534/1‑69 (MQ=255) aGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGAt > 1:1372783/1‑69 (MQ=255) aGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATc < 1:4143871/70‑1 (MQ=255) aGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATc < 1:160757/70‑1 (MQ=255) aGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATc < 1:695291/70‑1 (MQ=255) gTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAg > 1:2447617/1‑71 (MQ=255) aaTACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGc > 1:1320987/1‑55 (MQ=255) aaTACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAGTa > 1:2825192/1‑70 (MQ=255) aaTACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAGTa > 1:3092976/1‑70 (MQ=255) aaTACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAGTa > 1:1161707/1‑70 (MQ=255) aaTACCATCTTGATGATATATCCATGCACCGACTGCGTg > 1:2966078/1‑39 (MQ=255) aTACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAGTaca < 1:186883/71‑1 (MQ=255) | GGGTAAAACTCTGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAGCCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAGTACACGT > minE/1010758‑1010897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |