| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,026,348 | T→C | 50.9% | E175G (GAG→GGG) | malI ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,026,348 | 0 | T | C | 50.9% | 1.4 / 87.4 | 59 | E175G (GAG→GGG) | malI | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (19/10); new base C (18/12); total (37/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAAC > minE/1026283‑1026411 | gggTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGt > 1:3583634/1‑70 (MQ=255) gTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaa > 1:271981/1‑71 (MQ=255) aTGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTcaacaa < 1:1961596/70‑1 (MQ=255) aTGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTcaacaa < 1:1290582/70‑1 (MQ=255) tGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:4210461/67‑1 (MQ=255) tGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCaacaac < 1:2487733/70‑1 (MQ=255) gAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCaacaac < 1:1440574/69‑1 (MQ=255) gAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCaacaac < 1:3131168/69‑1 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCt > 1:3787400/1‑71 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGACCCGTCAACAACTGTGCAGCCt > 1:824013/1‑71 (MQ=255) tGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTg > 1:4020312/1‑71 (MQ=255) tGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTCCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTg > 1:3151506/1‑71 (MQ=255) cTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCaacaac > 1:1627740/1‑56 (MQ=255) cTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCaacaac > 1:2064429/1‑56 (MQ=255) cTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCaacaac > 1:238474/1‑56 (MQ=255) cTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCaacaac > 1:2423676/1‑56 (MQ=255) cccAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgtt > 1:3076593/1‑71 (MQ=255) cccAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgtt > 1:2816903/1‑71 (MQ=255) cccAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgtt > 1:2525853/1‑71 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgttg > 1:3873300/1‑71 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgttg > 1:2996312/1‑71 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgttg > 1:3012473/1‑71 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgttg > 1:2714990/1‑71 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgttg > 1:2734446/1‑71 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgttg > 1:926497/1‑71 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgttg > 1:1634613/1‑71 (MQ=255) cagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgttgt > 1:1195425/1‑71 (MQ=255) cagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgttgt < 1:3923287/71‑1 (MQ=255) gCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgtt < 1:2453813/62‑1 (MQ=255) gCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgtt < 1:3118746/62‑1 (MQ=255) gCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgtt < 1:3127952/62‑1 (MQ=255) cGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGa < 1:1882858/71‑1 (MQ=255) ccGCTGATGCCCATTGCGAATGAGATGCTCCGTcaa > 1:3796020/1‑36 (MQ=255) ccGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg > 1:783762/1‑71 (MQ=255) cGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg > 1:1011440/1‑70 (MQ=255) cGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg > 1:3268913/1‑70 (MQ=255) cGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg < 1:866351/70‑1 (MQ=255) cGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg < 1:2911882/70‑1 (MQ=255) cGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg > 1:4017126/1‑70 (MQ=255) cTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACt > 1:3122063/1‑38 (MQ=255) cTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACt > 1:3471943/1‑38 (MQ=255) aTTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat > 1:835874/1‑71 (MQ=255) aTTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat > 1:978809/1‑71 (MQ=255) aTTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat > 1:3677138/1‑71 (MQ=255) aTTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat > 1:2532058/1‑71 (MQ=255) aTTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat > 1:2418300/1‑71 (MQ=255) ttGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat > 1:597268/1‑70 (MQ=255) ttGCGAATGAGATGCCCCGTCAACAACTGTGCAGc < 1:2858441/35‑1 (MQ=255) ttGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgtt < 1:630991/44‑1 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:2512426/1‑69 (MQ=255) cGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:2821196/71‑1 (MQ=255) cGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:857092/71‑1 (MQ=255) cGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:2372203/71‑1 (MQ=255) cGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:1219397/71‑1 (MQ=255) aaTGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt < 1:2513739/71‑1 (MQ=255) aaTGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt < 1:4086059/71‑1 (MQ=255) aaTGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt < 1:1060264/71‑1 (MQ=255) gagaTGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCg > 1:3853379/1‑45 (MQ=255) gagaTGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAAc > 1:3876262/1‑71 (MQ=255) | GGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAAC > minE/1026283‑1026411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |