| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,027,446 | A→G | 46.2% | N134S (AAC→AGC) | malX → | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,027,446 | 0 | A | G | 46.2% | ‑0.2 / 34.4 | 26 | N134S (AAC→AGC) | malX | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Reads supporting (aligned to +/- strand): ref base A (6/8); new base G (8/4); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
CGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGG > minE/1027377‑1027509 | cgGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAAc < 1:1375958/71‑1 (MQ=255) tAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATc > 1:1912375/1‑71 (MQ=255) cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCagag < 1:183858/69‑1 (MQ=255) gTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt < 1:986705/70‑1 (MQ=255) tGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCtt > 1:1671853/1‑71 (MQ=255) aGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCTTCCAGAGCATTCTTGGGATCCAGt > 1:795129/1‑71 (MQ=255) aGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAg > 1:3726421/1‑70 (MQ=255) ccACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCggg < 1:2604603/71‑1 (MQ=255) ccACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCggg < 1:2557938/71‑1 (MQ=255) aCGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGAt > 1:4187461/1‑71 (MQ=255) gATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCt > 1:4035302/1‑71 (MQ=255) gATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCt > 1:3012254/1‑71 (MQ=255) gATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCt > 1:2080647/1‑71 (MQ=255) aTGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTc < 1:1337276/71‑1 (MQ=255) cgGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggtgc > 1:1191485/1‑70 (MQ=255) ggTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggt > 1:1742103/1‑67 (MQ=255) ttCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggtgcggtg < 1:2209764/71‑1 (MQ=255) tCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGa > 1:2072389/1‑71 (MQ=255) cTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGAt > 1:2218339/1‑71 (MQ=255) cTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGAt > 1:3926967/1‑71 (MQ=255) aaaGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCt > 1:3861228/1‑56 (MQ=255) aaaGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATc < 1:2434091/69‑1 (MQ=255) aaaGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCg < 1:770954/70‑1 (MQ=255) gCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCgg < 1:2048462/71‑1 (MQ=255) gCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCgg < 1:4111216/71‑1 (MQ=255) gCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCgg < 1:1511400/71‑1 (MQ=255) aaCATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:3546780/1‑35 (MQ=255) aaCATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:696260/1‑35 (MQ=255) aaCATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:1450669/1‑35 (MQ=255) | CGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGG > minE/1027377‑1027509 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |