| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,069,991 | G→A | 41.3% | Q296Q (CAG→CAA) | cfa → | cyclopropane fatty acyl phospholipid synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,069,991 | 0 | G | A | 41.3% | 27.7 / 53.3 | 46 | Q296Q (CAG→CAA) | cfa | cyclopropane fatty acyl phospholipid synthase |
| Reads supporting (aligned to +/- strand): ref base G (16/11); new base A (6/13); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.97e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTACGATA > minE/1069929‑1070058 | aaTGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGc < 1:1673151/68‑1 (MQ=255) ttGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTc > 1:3640914/1‑70 (MQ=255) ttGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTcc < 1:3948230/71‑1 (MQ=255) tGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTcc < 1:3622334/70‑1 (MQ=255) gATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCAg > 1:2374054/1‑71 (MQ=255) gATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCAg > 1:3695413/1‑71 (MQ=255) tCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCg < 1:3078362/71‑1 (MQ=255) ccTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGaa > 1:3206275/1‑71 (MQ=255) aTTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAAcccc < 1:265505/70‑1 (MQ=255) atatatTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGa < 1:2249932/71‑1 (MQ=255) atatatTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGa < 1:2433358/71‑1 (MQ=255) atatTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGAt > 1:1474823/1‑70 (MQ=255) atatTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATg > 1:1250107/1‑71 (MQ=255) atatTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATg > 1:2481617/1‑71 (MQ=255) tCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGAc < 1:46872/70‑1 (MQ=255) tCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACt > 1:927992/1‑71 (MQ=255) aaCGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTtgt < 1:3765886/56‑1 (MQ=255) aCGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCAt < 1:2673283/71‑1 (MQ=255) gTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTTGCATAAc > 1:579101/1‑71 (MQ=255) gTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAAc > 1:2848247/1‑71 (MQ=255) gTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAAc < 1:2792848/71‑1 (MQ=255) gTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTAGTGATGGAAGACTGGCATAAc < 1:94969/71‑1 (MQ=255) tgcctgccCTCTGTACGCCAGATTGGTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACtt > 1:3477367/1‑71 (MQ=255) tgcctgccCTCTGTACGCCAGATTGCTCAGTCCAg > 1:3391092/1‑35 (MQ=255) tgcctgccCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTtgt < 1:99536/50‑1 (MQ=255) tgcctgccCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACtt > 1:1768587/1‑71 (MQ=255) gcctgccCTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACtt < 1:2066561/70‑1 (MQ=255) gcctgccCTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACtt < 1:2006838/70‑1 (MQ=255) gcctgccCTCTGTACGCCAAATTGCGCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACtt < 1:2086606/70‑1 (MQ=255) ctgccCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCgg < 1:2200208/71‑1 (MQ=255) ctgccCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCgg < 1:732075/71‑1 (MQ=255) ctgccCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCgg < 1:4228128/71‑1 (MQ=255) ctgccCTCTGTACGCCAGATTGCTCAGTCAAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCgg < 1:930987/71‑1 (MQ=255) cccTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTg < 1:2237695/70‑1 (MQ=255) cccTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTg < 1:3652237/70‑1 (MQ=255) ctGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGAt > 1:1721024/1‑71 (MQ=255) ctGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGAt > 1:494177/1‑71 (MQ=255) ctGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGAt > 1:719611/1‑71 (MQ=255) ctGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGa > 1:476492/1‑70 (MQ=255) tGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATTGAAGACTGGCATAACTTCGGTGCTGa < 1:3822621/69‑1 (MQ=255) cGCCAAATTACTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTACGa < 1:1598425/71‑1 (MQ=255) ccAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTACGAta > 1:3154516/1‑71 (MQ=255) ccAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTACGAta > 1:1292952/1‑71 (MQ=255) cAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACt > 1:2489130/1‑43 (MQ=255) cAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACt > 1:1623052/1‑43 (MQ=255) cAGATTGCCCAGTCCAGCGAACCCCACTTTGTGATGGAAGACt > 1:3550658/1‑43 (MQ=255) | AATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTACGATA > minE/1069929‑1070058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |