| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,073,671 | C→T | 52.2% | G50S (GGC→AGC) | ydhQ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,073,671 | 0 | C | T | 52.2% | ‑2.2 / 68.4 | 46 | G50S (GGC→AGC) | ydhQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (13/9); new base T (12/12); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAG > minE/1073608‑1073737 | gCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGccccc < 1:2397636/71‑1 (MQ=255) gTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGt < 1:144348/71‑1 (MQ=255) gTTACCTTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACgg > 1:543221/1‑71 (MQ=255) gTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACgg > 1:2980144/1‑71 (MQ=255) gTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACgg > 1:2223901/1‑71 (MQ=255) gTTACCGTACTGTGTGCATTTGGCGATCACACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGAc > 1:725246/1‑66 (MQ=255) tgtgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGa > 1:3383224/1‑53 (MQ=255) tgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGctct < 1:619429/70‑1 (MQ=255) tgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGctct < 1:3429221/70‑1 (MQ=255) tgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGctct < 1:2954292/70‑1 (MQ=255) tgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCtt > 1:2171572/1‑71 (MQ=255) ggCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAg > 1:1809685/1‑71 (MQ=255) ggCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAg > 1:255833/1‑71 (MQ=255) aTCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCtt > 1:206287/1‑70 (MQ=255) aTCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTc < 1:1179824/71‑1 (MQ=255) tCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTc < 1:2210995/52‑1 (MQ=255) ccACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCgg > 1:2189993/1‑52 (MQ=255) aCCGGTTGGCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTca < 1:3715089/67‑1 (MQ=255) aCCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGctct < 1:1965587/54‑1 (MQ=255) aCCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGctct < 1:3641684/54‑1 (MQ=255) aCCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGctct < 1:3061302/54‑1 (MQ=255) aCCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTca < 1:589720/67‑1 (MQ=255) aCCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTca < 1:42984/67‑1 (MQ=255) cGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTc > 1:1947282/1‑54 (MQ=255) cGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTc > 1:1382276/1‑54 (MQ=255) cGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTc > 1:1407200/1‑54 (MQ=255) cGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTc > 1:178240/1‑54 (MQ=255) cGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTc > 1:2738171/1‑54 (MQ=255) cGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTc > 1:2405602/1‑54 (MQ=255) ggTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAAt > 1:1253255/1‑69 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:2814756/62‑1 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:753228/62‑1 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:748062/62‑1 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:1013663/62‑1 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:2401714/62‑1 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:1408045/1‑71 (MQ=255) cTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:1868141/1‑71 (MQ=255) tttCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTc > 1:3108839/1‑44 (MQ=255) ttCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt > 1:2944242/1‑70 (MQ=255) ttCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt > 1:3264763/1‑70 (MQ=255) ttCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt > 1:320174/1‑70 (MQ=255) tCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt > 1:1395487/1‑69 (MQ=255) tCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt > 1:2600820/1‑69 (MQ=255) aCCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTa < 1:4209414/71‑1 (MQ=255) aCCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTa < 1:1863610/71‑1 (MQ=255) aCCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTa < 1:1915705/71‑1 (MQ=255) ccagctagCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGtt < 1:1822781/70‑2 (MQ=255) cagctagcCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAg > 1:347081/1‑71 (MQ=255) | GCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAG > minE/1073608‑1073737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |