| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 199,135 | T→A | 35.4% | S207C (AGC→TGC) | metQ ← | DL‑methionine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 199,135 | 0 | T | A | 35.4% | 32.1 / 25.8 | 31 | S207C (AGC→TGC) | metQ | DL‑methionine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (16/4); new base A (7/4); total (23/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTTG > minE/199067‑199198 | gTTTACGTACGGTGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctgg < 1:380518/71‑1 (MQ=255)gTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctgg < 1:848924/71‑1 (MQ=255) acgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtg > 1:2727212/1‑71 (MQ=255) ctctTTATCTTCAACAAAGATACCGTCTTTCGCCGTAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGa > 1:3365771/1‑71 (MQ=255) ctctTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGa > 1:3751760/1‑71 (MQ=255) ctctTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGa > 1:2823891/1‑71 (MQ=255) ctctTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGa < 1:4029020/71‑1 (MQ=255) tttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggcagg < 1:2561386/53‑1 (MQ=255) tttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATaa > 1:3800005/1‑71 (MQ=255) ttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCa > 1:4026601/1‑54 (MQ=255) tATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACt > 1:3792517/1‑71 (MQ=255) tATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACt > 1:3146616/1‑71 (MQ=255) tATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACt > 1:3263347/1‑71 (MQ=255) tCTTCAACAAAGATACCGTCTTTAGCCGGAGTCAGGCCAATctggctg > 1:1030356/1‑48 (MQ=255) ttCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGAt < 1:444026/63‑1 (MQ=255) ttCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGAt < 1:505073/63‑1 (MQ=255) caacaaAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTg > 1:2469795/1‑66 (MQ=255) caacaaAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGc < 1:3833012/67‑1 (MQ=255) aaaGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGa > 1:2832995/1‑71 (MQ=255) aaGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGAt > 1:1273133/1‑71 (MQ=255) aCCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg > 1:2770637/1‑40 (MQ=255) tCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACTGCCAGAGCGAtt > 1:2271349/1‑59 (MQ=255) tCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCgtcgtc > 1:1425883/1‑70 (MQ=255) tCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCgtcgtc > 1:3843395/1‑70 (MQ=255) cGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCgtcgtc > 1:2807230/1‑69 (MQ=255) ggAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCagag > 1:3603288/1‑70 (MQ=255) ggAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCagag > 1:718361/1‑70 (MQ=255) ggAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCagag > 1:2288593/1‑70 (MQ=255) gTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAAcg > 1:2388588/1‑71 (MQ=255) gTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAAcg > 1:2904717/1‑71 (MQ=255) gTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTAGTCCAGAGAAcg > 1:1930932/1‑71 (MQ=255) gTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAAcg < 1:3567915/71‑1 (MQ=255) gCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCa < 1:3271733/71‑1 (MQ=255) tCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTTg < 1:765048/70‑1 (MQ=255) tCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTTg < 1:3768004/70‑1 (MQ=255) ctggctggCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCaga > 1:1759224/1‑55 (MQ=255) ctggctggCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCaga > 1:3002659/1‑55 (MQ=255) ctggctggCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCaga > 1:25348/1‑55 (MQ=255) ctggctggCAT‑‑GTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCaga > 1:1192729/1‑53 (MQ=255) | GTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTTG > minE/199067‑199198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |