| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,094,567 | T→C | 58.7% | I604V (ATT→GTT) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,094,567 | 0 | T | C | 58.7% | 27.7 / 53.9 | 46 | I604V (ATT→GTT) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (7/12); new base C (13/14); total (20/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
CCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAAC > minE/1094513‑1094632 | ccAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCgg > 1:2987610/1‑59 (MQ=255)ccAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCgg > 1:1288365/1‑59 (MQ=255) aacaaACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCtt < 1:619404/71‑1 (MQ=255) acaaACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGtt < 1:299608/58‑1 (MQ=255) acaaACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCAGGGTGATCTTc < 1:1350786/71‑1 (MQ=255) acaaACGCAGCCATTCACGCACAAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTc < 1:1385204/71‑1 (MQ=255) cGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGa > 1:2068314/1‑71 (MQ=255) cGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGa > 1:1831217/1‑71 (MQ=255) ttCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATc < 1:1715451/61‑1 (MQ=255) ttCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATc < 1:2310988/61‑1 (MQ=255) ttCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATc < 1:4111489/61‑1 (MQ=255) ttCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATc < 1:3759282/61‑1 (MQ=255) ttCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATc < 1:2457393/61‑1 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:3063318/1‑71 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCg > 1:3476512/1‑40 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGAcaca > 1:3330596/1‑69 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:988042/1‑71 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:2695489/1‑71 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:1930398/1‑71 (MQ=255) aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:987502/71‑1 (MQ=255) aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:1043946/71‑1 (MQ=255) aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:3278789/71‑1 (MQ=255) cGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCgg < 1:693064/71‑1 (MQ=255) aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGaca > 1:2183669/1‑59 (MQ=255) aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGaca > 1:2311025/1‑59 (MQ=255) aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGaca > 1:1007217/1‑59 (MQ=255) aGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACg > 1:3172069/1‑71 (MQ=255) ttGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGAc < 1:1850321/63‑1 (MQ=255) tGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCAt > 1:1473482/1‑71 (MQ=255) gcgcgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCtt > 1:3289881/1‑41 (MQ=255) gcgcgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCtt > 1:3924130/1‑41 (MQ=255) gcgcgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCtt > 1:2781713/1‑41 (MQ=255) cgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCtt < 1:521392/40‑1 (MQ=255) cTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:3566231/71‑1 (MQ=255) cTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:200068/71‑1 (MQ=255) cTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:1130707/71‑1 (MQ=255) cTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:1034554/71‑1 (MQ=255) cTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:1115144/71‑1 (MQ=255) tttCGGTGAATGAATCCGTTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCaa < 1:2371882/65‑1 (MQ=255) tttCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCaa < 1:2539130/65‑1 (MQ=255) tttCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCaa < 1:2426644/65‑1 (MQ=255) tttCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCaa < 1:3699167/65‑1 (MQ=255) tttCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCaa < 1:573325/65‑1 (MQ=255) tttCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg > 1:1858858/1‑70 (MQ=255) tCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:788396/1‑71 (MQ=255) ggTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCa < 1:2508514/71‑1 (MQ=255) aatgaatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATaaa < 1:2867156/71‑1 (MQ=255) aatgaatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATaaa < 1:2143892/71‑1 (MQ=255) aatgaacCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATaa < 1:806690/70‑1 (MQ=255) aatgaacCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATaa < 1:938608/70‑1 (MQ=255) atgaatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATaaa < 1:3110028/70‑1 (MQ=255) atgaatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAAc < 1:455473/71‑1 (MQ=255) | CCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAAC > minE/1094513‑1094632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |