| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,104,294 | A→T | 10.2% | G339G (GGA→GGT) | ydiF → | fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit and beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,104,294 | 0 | A | T | 10.2% | 130.5 / 7.7 | 49 | G339G (GGA→GGT) | ydiF | fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit and beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (22/22); new base T (2/3); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACG > minE/1104232‑1104357 | gCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTc < 1:3803820/71‑1 (MQ=255)gCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTc < 1:3234401/71‑1 (MQ=255) gaagaaGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGTATTACTTca < 1:274938/66‑1 (MQ=255) gaagaaGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGTATTACTTca < 1:1577789/66‑1 (MQ=255) gaagaaGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGTATTACTTca < 1:3675666/66‑1 (MQ=255) agaagGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGTATTACTTcaca > 1:3293747/1‑66 (MQ=255) agaagGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGTATTACTTcaca > 1:1600240/1‑66 (MQ=255) aagGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATc < 1:3440463/71‑1 (MQ=255) ggTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGc < 1:98194/71‑1 (MQ=255) gtgCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGaa > 1:3685791/1‑48 (MQ=255) cTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGgc > 1:608984/1‑71 (MQ=255) gatgaCTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCttt < 1:4049897/66‑1 (MQ=255) tgaCTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGcgcg > 1:2134421/1‑70 (MQ=255) tgaCTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGcgcg > 1:1388435/1‑70 (MQ=255) gaCTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTcaca > 1:2114004/1‑50 (MQ=255) gaCTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAg > 1:2292029/1‑51 (MQ=255) aCTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAggg > 1:2674863/1‑52 (MQ=255) tttATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGt > 1:1749346/1‑71 (MQ=255) tttATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGt > 1:1243626/1‑71 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGTATCGCCTTTGGCGCGAACGTg < 1:1137005/70‑1 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACTTg < 1:602060/70‑1 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTg < 1:4057134/70‑1 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTg < 1:229905/70‑1 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTg < 1:3209840/70‑1 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTg > 1:296514/1‑70 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTg < 1:2890179/70‑1 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTg > 1:1893764/1‑70 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTg > 1:98627/1‑70 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGAGCGCCTTTGGCGCGAACGt < 1:2077400/69‑1 (MQ=255) tATTCTGACGGTAGAAACAGGTCCGATTGG‑GGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGt < 1:1670817/68‑1 (MQ=255) aTTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGc < 1:931635/53‑1 (MQ=255) ttCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGaa < 1:4094874/70‑1 (MQ=255) ttCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGaa < 1:1581941/70‑1 (MQ=255) gACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCtt > 1:1681277/1‑51 (MQ=255) gACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATAccc > 1:3214547/1‑71 (MQ=255) gACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATAccc > 1:1233271/1‑71 (MQ=255) gACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATAcc > 1:3455194/1‑70 (MQ=255) gTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGaa < 1:4255199/56‑1 (MQ=255) aGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCa > 1:453735/1‑71 (MQ=255) aGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCa > 1:2410247/1‑71 (MQ=255) ggTCCGATTGGCGGAATTACTTCACACGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGa < 1:3666696/71‑1 (MQ=255) gTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTg > 1:3874960/1‑68 (MQ=255) gTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTg > 1:3295561/1‑68 (MQ=255) cGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGatat < 1:4183701/70‑1 (MQ=255) cGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGatat < 1:3072258/70‑1 (MQ=255) cGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGatat < 1:1707606/70‑1 (MQ=255) aTTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGa > 1:406936/1‑70 (MQ=255) ttGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACg > 1:4023923/1‑71 (MQ=255) ttGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACg < 1:1489000/71‑1 (MQ=255) ggCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGa > 1:3886266/1‑62 (MQ=255) | GCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACG > minE/1104232‑1104357 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |