| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,111,527 | G→A | 50.9% | C287Y (TGT→TAT) | ydiD → | short chain acyl‑CoA synthetase, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,111,527 | 0 | G | A | 50.9% | ‑5.4 / 87.7 | 57 | C287Y (TGT→TAT) | ydiD | short chain acyl‑CoA synthetase, anaerobic |
| Reads supporting (aligned to +/- strand): ref base G (11/17); new base A (12/17); total (23/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATG > minE/1111458‑1111589 | aTGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCgtgt > 1:1232559/1‑71 (MQ=255)aTGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCgtgt > 1:618429/1‑71 (MQ=255)aTGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCgtgt > 1:2305196/1‑71 (MQ=255) tGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGtc < 1:770194/71‑1 (MQ=255) tGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGtc < 1:2783396/71‑1 (MQ=255) tGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGtc < 1:1803614/71‑1 (MQ=255) aCGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCgcgc < 1:3516118/70‑1 (MQ=255) aCGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCgct > 1:1105505/1‑71 (MQ=255) cGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCgctg < 1:93406/71‑1 (MQ=255) cGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCgctg < 1:390015/71‑1 (MQ=255) cGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCgctg < 1:2670409/71‑1 (MQ=255) ggCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCgctgc < 1:30475/71‑1 (MQ=255) aCCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCtt > 1:2381409/1‑70 (MQ=255) ccATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCtt > 1:3263761/1‑69 (MQ=255) ccATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGAGCTGCtt > 1:4155566/1‑69 (MQ=255) tCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagcag > 1:3750998/1‑71 (MQ=255) tCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagcag > 1:344337/1‑71 (MQ=255) ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGt < 1:2196044/71‑1 (MQ=255) gcgcTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGc < 1:762860/71‑1 (MQ=255) gcgcTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGc < 1:327299/71‑1 (MQ=255) gcgcTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGc < 1:2629447/71‑1 (MQ=255) cGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcagc > 1:1048711/1‑58 (MQ=255) cGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTg > 1:4255659/1‑71 (MQ=255) tgttgtTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGag < 1:753531/47‑1 (MQ=255) tgttgtTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGag < 1:3670826/47‑1 (MQ=255) tgttgtTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGag < 1:467164/47‑1 (MQ=255) tgttgtTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGag < 1:3118498/47‑1 (MQ=255) tgttgtTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGag < 1:1604899/47‑1 (MQ=255) tgttgtTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGag < 1:3994946/47‑1 (MQ=255) tgttgtTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGag < 1:3447444/47‑1 (MQ=255) tgttggTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGag < 1:1537678/47‑1 (MQ=255) tgttggTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGag < 1:4131458/47‑1 (MQ=255) ttAGATATTTTCACTCCTGATGCGTGTCTCGCgctg < 1:375324/36‑1 (MQ=255) ttAGATATTTTCACTCCTGATGCGTGTCTCGCgctg < 1:488227/36‑1 (MQ=255) ttAGATATTTTCACTCCTGATGCGTGTCTCGCgctg < 1:3848766/36‑1 (MQ=255) gATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCaa < 1:1784139/70‑1 (MQ=255) gATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCaa < 1:3220988/70‑1 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGt > 1:1957769/1‑45 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGt > 1:2424609/1‑45 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGcc > 1:1713707/1‑71 (MQ=255) atTTTCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGt > 1:3873954/1‑45 (MQ=255) atTTTCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGt > 1:2120086/1‑45 (MQ=255) atTTTCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGt > 1:2855657/1‑45 (MQ=255) atTTTCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGAAGCGt > 1:3346626/1‑45 (MQ=255) ttCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGtt > 1:3108377/1‑71 (MQ=255) ttCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGtt > 1:3103207/1‑71 (MQ=255) ttCACTCCTGATGCGTATGTCGCGCTGCTTGAGCAGCAGCGTTGc > 1:2308018/1‑45 (MQ=255) ttCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGc > 1:1574305/1‑45 (MQ=255) ttCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGtt > 1:2848793/1‑71 (MQ=255) tCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGtt < 1:2009287/70‑1 (MQ=255) cTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTc < 1:634345/71‑1 (MQ=255) ccTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTa > 1:3537289/1‑71 (MQ=255) ccTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTa > 1:2257573/1‑71 (MQ=255) ccTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTa > 1:1566793/1‑71 (MQ=255) cTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCt < 1:3162018/69‑1 (MQ=255) cTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCt < 1:4242305/69‑1 (MQ=255) cTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCt < 1:2046753/69‑1 (MQ=255) aTGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATg < 1:3723892/69‑1 (MQ=255) aTGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATg < 1:3906934/69‑1 (MQ=255) aTGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGGTTGTCTATg < 1:198754/69‑1 (MQ=255) | ATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATG > minE/1111458‑1111589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |