| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,142,011 | T→C | 43.0% | F152L (TTC→CTC) | katE → | hydroperoxidase HPII(III) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,142,011 | 0 | T | C | 43.0% | 30.0 / 105.7 | 79 | F152L (TTC→CTC) | katE | hydroperoxidase HPII(III) |
| Reads supporting (aligned to +/- strand): ref base T (25/20); new base C (24/10); total (49/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
CACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGGCT > minE/1141943‑1142080 | cACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGAttt < 1:898549/70‑1 (MQ=255) gcgGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCctc < 1:277302/71‑1 (MQ=255) ggATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCtctc < 1:3559812/71‑1 (MQ=255) ggATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCtctc < 1:4056383/71‑1 (MQ=255) gATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCtctc < 1:4208560/70‑1 (MQ=255) aTCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCctct > 1:234748/1‑68 (MQ=255) tCAGACGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGa < 1:3146878/71‑1 (MQ=255) gCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATcc > 1:10923/1‑71 (MQ=255) ggTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACGAAAGCGGATCTCCTCTCAGATCCGAACAAAAt < 1:3397597/71‑1 (MQ=255) ggTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAAt < 1:2424611/71‑1 (MQ=255) ggTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAAt < 1:2893070/71‑1 (MQ=255) ggTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAAt < 1:1940564/71‑1 (MQ=255) ggTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAAt < 1:3506550/71‑1 (MQ=255) gTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCctct < 1:1453334/54‑1 (MQ=255) gTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAAt > 1:481914/1‑70 (MQ=255) ttATTTCCAGCCATATAAAAGCTTAAGCGATATTAACAAAGCGGATTTCCTCTCAGATCCGAACAAAATCa > 1:559062/1‑71 (MQ=255) aTTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCAc < 1:3404247/70‑1 (MQ=255) tCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCAccc > 1:3416768/1‑69 (MQ=255) tCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCAccc > 1:3680554/1‑69 (MQ=255) tCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCAccc > 1:366770/1‑69 (MQ=255) cAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAg < 1:828631/70‑1 (MQ=255) cAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGt < 1:1033251/71‑1 (MQ=255) ccATATAAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAATATCACCCCAGTAtt < 1:2917237/71‑1 (MQ=255) cATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTAttt > 1:2278063/1‑71 (MQ=255) cATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTAttt > 1:4188012/1‑71 (MQ=255) tataAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTg > 1:2172604/1‑70 (MQ=255) tataAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTg > 1:2934079/1‑70 (MQ=255) tataAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACaa < 1:658434/53‑1 (MQ=255) tataAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACaa < 1:923981/53‑1 (MQ=255) ataAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCa > 1:3748467/1‑57 (MQ=255) ataAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCa > 1:3954321/1‑57 (MQ=255) ataAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCa > 1:2580506/1‑57 (MQ=255) ataAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCa > 1:4155553/1‑57 (MQ=255) taAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAAt > 1:4051210/1‑54 (MQ=255) taAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAAt > 1:1135864/1‑54 (MQ=255) taAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAAt > 1:2972880/1‑54 (MQ=255) taAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTAc > 1:1899065/1‑71 (MQ=255) taAAAGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTAc > 1:1739771/1‑71 (MQ=255) taAAAGCTTAAGCGATATTACAAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTAc > 1:952357/1‑71 (MQ=255) aaaGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCa > 1:1505057/1‑38 (MQ=255) aaaGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTAc > 1:1654137/1‑69 (MQ=255) aGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCAcccc > 1:834140/1‑56 (MQ=255) aGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCa > 1:2013382/1‑57 (MQ=255) aGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCa > 1:2915751/1‑57 (MQ=255) aGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCa > 1:2111813/1‑57 (MQ=255) aGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCa > 1:4228868/1‑57 (MQ=255) aGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTtc < 1:2574749/66‑1 (MQ=255) aGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTtc < 1:1921484/66‑1 (MQ=255) aGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTtc < 1:521003/66‑1 (MQ=255) cGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTtctc > 1:2959739/1‑66 (MQ=255) atatTACCAAAGCGGATTTCCTCTCAGATCCGAACaa < 1:3600580/37‑1 (MQ=255) atatTACCAAAGCGGATTTCCTCTCAGATCCGAACaa < 1:4070408/37‑1 (MQ=255) atatTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGtt > 1:2701362/1‑71 (MQ=255) atatTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGtt > 1:4091019/1‑71 (MQ=255) tatTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc < 1:2650730/71‑1 (MQ=255) tatTACCAAAGCGGATCTCCTCTCAGATCCGAACaaaa > 1:4256960/1‑38 (MQ=255) tatTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGtt > 1:3545106/1‑70 (MQ=255) tatTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGtt > 1:1219898/1‑70 (MQ=255) atTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGtt > 1:3765941/1‑69 (MQ=255) ttACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCa < 1:1876306/70‑1 (MQ=255) ttACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCa < 1:1503252/70‑1 (MQ=255) ttACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCa < 1:2727012/70‑1 (MQ=255) ttACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAg > 1:3891156/1‑71 (MQ=255) ttACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAg > 1:3597227/1‑71 (MQ=255) tACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTa > 1:2231264/1‑52 (MQ=255) tACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTa > 1:3995852/1‑52 (MQ=255) tACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTa > 1:1423111/1‑52 (MQ=255) aCCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAggg > 1:1363599/1‑71 (MQ=255) aCCAAAGCGGATTTCATCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAggg > 1:1078876/1‑71 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCa > 1:2873102/1‑37 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAggg > 1:1069772/1‑71 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAggg > 1:947060/1‑71 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAggg > 1:3640966/1‑71 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAggg > 1:2478900/1‑71 (MQ=255) aGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACg > 1:3198540/1‑48 (MQ=255) gATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGt < 1:3251686/45‑1 (MQ=255) gATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGt < 1:3039210/45‑1 (MQ=255) gATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGt < 1:2646213/45‑1 (MQ=255) gATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGt < 1:1709155/45‑1 (MQ=255) gATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTgg > 1:2145075/1‑71 (MQ=255) tttCCTCTCAGATCCGAACAAAATCACCCCAGTAt < 1:3169136/35‑1 (MQ=255) tttCCTCTCAGATCCGAACAAAATCACCCCAGTAt < 1:340999/35‑1 (MQ=255) tttCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGGct > 1:2039035/1‑71 (MQ=255) | CACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGGCT > minE/1141943‑1142080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |