| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,142,959 | A→G | 57.1% | N468D (AAC→GAC) | katE → | hydroperoxidase HPII(III) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,142,959 | 0 | A | G | 57.1% | 27.7 / 69.9 | 56 | N468D (AAC→GAC) | katE | hydroperoxidase HPII(III) |
| Reads supporting (aligned to +/- strand): ref base A (15/9); new base G (15/17); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01 | |||||||||||
CATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTGGAA > minE/1142902‑1143027 | catCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAAc > 1:2976865/1‑60 (MQ=255)catCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAAc > 1:3986136/1‑60 (MQ=255)catCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAAc > 1:2601696/1‑60 (MQ=255)catCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAAc > 1:2538630/1‑60 (MQ=255)catCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGa < 1:959948/71‑1 (MQ=255)catCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGa < 1:645508/71‑1 (MQ=255)catCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGa < 1:13538/71‑1 (MQ=255)catCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGa < 1:3360789/71‑1 (MQ=255) tGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACAccg > 1:2567571/1‑71 (MQ=255) tGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACAcc > 1:1047140/1‑70 (MQ=255) cacTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGAGGCCGa < 1:545802/71‑1 (MQ=255) acTAACCCGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAacac > 1:451675/1‑58 (MQ=255) acTAACCCGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAacac > 1:1464009/1‑58 (MQ=255) acTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGaaa > 1:1809419/1‑55 (MQ=255) acTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGaaa > 1:1841420/1‑55 (MQ=255) acTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGaaa > 1:3126520/1‑55 (MQ=255) acTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGaaa > 1:3389379/1‑55 (MQ=255) cTAACCCGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGa < 1:3065922/69‑1 (MQ=255) cTAACCCGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGa < 1:4238937/69‑1 (MQ=255) cTAACCCGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGa < 1:1404550/69‑1 (MQ=255) tAACCCGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAAc > 1:832044/1‑71 (MQ=255) aaCCCGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:2883381/1‑69 (MQ=255) ggCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACgcggcg > 1:3261768/1‑71 (MQ=255) gAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGtt > 1:3351528/1‑71 (MQ=255) aTTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGtttt < 1:4148347/71‑1 (MQ=255) aTTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGtttt < 1:1819392/71‑1 (MQ=255) ttACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAac < 1:3189392/42‑1 (MQ=255) ttACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAac < 1:1011278/42‑1 (MQ=255) ttACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAac < 1:988799/42‑1 (MQ=255) ttACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAac < 1:2979928/42‑1 (MQ=255) ttACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAac < 1:210044/42‑1 (MQ=255) ttACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAac < 1:1935728/42‑1 (MQ=255) ttACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTg < 1:596523/71‑1 (MQ=255) tACGAACCGAACTCGATTAACGATAACTGGCCGCGCGaaa > 1:2184110/1‑40 (MQ=255) tACGAACCGAACTCGATTAACGATAACTGGCCGCGCGaaa > 1:1573141/1‑40 (MQ=255) cgaaccgaacTCGATTAACGATGACTGGCCGCGCGaaa > 1:1179470/1‑38 (MQ=255) cgaaccgaacTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt < 1:1844072/71‑1 (MQ=255) cgaacTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTg < 1:3644605/63‑1 (MQ=255) cgaacTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATa < 1:3958656/70‑1 (MQ=255) cgaacTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATAc > 1:1237093/1‑71 (MQ=255) cgaacTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATAc > 1:2355720/1‑71 (MQ=255) cgaacTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATAc > 1:4035914/1‑71 (MQ=255) cgaacTCGATAAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATa < 1:3953547/70‑1 (MQ=255) gaacTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATAcc < 1:1691895/71‑1 (MQ=255) gaacTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATAc > 1:4019383/1‑70 (MQ=255) gaacTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATAc > 1:3966013/1‑70 (MQ=255) gaacTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATAc > 1:2294305/1‑70 (MQ=255) aacTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATAc > 1:4087349/1‑69 (MQ=255) ttAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:3093958/1‑41 (MQ=255) ttAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:495706/1‑41 (MQ=255) ttAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:196239/1‑41 (MQ=255) ttAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAgcg < 1:2194170/70‑1 (MQ=255) ttAACGATGACTGCCCGCGCGAAACACCGCCTGGGCCGaaa > 1:2825563/1‑41 (MQ=37) cGATGACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTgg < 1:1175587/71‑1 (MQ=255) gATGACTGGCCGCGCGAAACACCTCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTgg < 1:1335123/70‑1 (MQ=255) aTAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTGGaa < 1:3096809/71‑1 (MQ=255) | CATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTGGAA > minE/1142902‑1143027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |