| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,179,007 | A→T | 13.2% | N153Y (AAC→TAC) | ansA → | cytoplasmic L‑asparaginase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,179,007 | 0 | A | T | 13.2% | 236.8 / 27.6 | 91 | N153Y (AAC→TAC) | ansA | cytoplasmic L‑asparaginase I |
| Reads supporting (aligned to +/- strand): ref base A (46/33); new base T (8/4); total (54/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGCC > minE/1178938‑1179069 | aTTAATCTGCTGAATGCGTTGTAGGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTT‑AATAAc > 1:1513353/1‑71 (MQ=255)aTTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATaa > 1:795734/1‑71 (MQ=255) ttAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATa < 1:3915788/69‑1 (MQ=255) ttAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATa < 1:3183194/69‑1 (MQ=255) ttAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATa < 1:2769790/69‑1 (MQ=255) ttAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATa < 1:4116194/69‑1 (MQ=255) ttAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATa < 1:3599800/69‑1 (MQ=255) tAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc > 1:2297348/1‑71 (MQ=255) tAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc > 1:2956763/1‑71 (MQ=255) aaTCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc > 1:1229299/1‑70 (MQ=255) aaTCTGATGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc > 1:1525473/1‑70 (MQ=255) ttCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCg < 1:2802561/69‑1 (MQ=255) aTCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc < 1:2364612/69‑1 (MQ=255) aTCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc < 1:1892859/69‑1 (MQ=255) tCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc < 1:874624/68‑1 (MQ=255) tgctgAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTg > 1:2286952/1‑71 (MQ=255) ctgAATGCGT‑‑TACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTAt < 1:2047058/70‑1 (MQ=255) aaTGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTAt < 1:1389161/69‑1 (MQ=255) aaTGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTAt < 1:876272/69‑1 (MQ=255) aaTGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTAt < 1:377216/69‑1 (MQ=255) aaTGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATc < 1:2747075/70‑1 (MQ=255) aaTGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATc < 1:3629484/70‑1 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgcg > 1:3089551/1‑71 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgcg < 1:2047543/71‑1 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgcg > 1:3763190/1‑71 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgcg > 1:3632822/1‑71 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgcg < 1:2470867/71‑1 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgcg < 1:1345119/71‑1 (MQ=255) gTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATa < 1:3199290/53‑1 (MQ=255) gTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGc > 1:3964609/1‑70 (MQ=255) gTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGc > 1:1320892/1‑70 (MQ=255) gTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGc > 1:3356041/1‑70 (MQ=255) gTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGc > 1:2685330/1‑70 (MQ=255) gTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGa > 1:168655/1‑55 (MQ=255) gTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGa > 1:317933/1‑55 (MQ=255) tACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCg > 1:3453219/1‑71 (MQ=255) tACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCg < 1:3396130/71‑1 (MQ=255) tACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCg < 1:3865602/71‑1 (MQ=255) tACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCg > 1:4086511/1‑71 (MQ=255) tACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCg > 1:814275/1‑71 (MQ=255) cGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGc < 1:3697090/70‑1 (MQ=255) cGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCa > 1:2842337/1‑71 (MQ=255) ttGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTAt > 1:1234820/1‑56 (MQ=255) ttGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTAt > 1:3419944/1‑56 (MQ=255) gcgAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCaa < 1:840735/71‑1 (MQ=255) aTTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGcc > 1:2405343/1‑71 (MQ=255) ttATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc < 1:878824/38‑1 (MQ=255) aTCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCaa > 1:581527/1‑52 (MQ=255) tCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATg > 1:3862971/1‑71 (MQ=255) tCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATg > 1:106255/1‑71 (MQ=255) cGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGcc < 1:856121/71‑1 (MQ=255) cGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGcc < 1:1008792/71‑1 (MQ=255) cAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGc > 1:741652/1‑43 (MQ=255) cAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGc > 1:4186684/1‑43 (MQ=255) cAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATg > 1:279638/1‑71 (MQ=255) gAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCaaa < 1:995845/54‑1 (MQ=255) gAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCaaa < 1:3154332/54‑1 (MQ=255) gAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCaaa < 1:2125464/54‑1 (MQ=255) aaGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCTCACTACCAAAGCCCATGCCGATGGtttt < 1:1486129/71‑1 (MQ=255) aaGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGtttt < 1:3233328/71‑1 (MQ=255) aGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCa > 1:3455228/1‑44 (MQ=255) aGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGtttt > 1:3296177/1‑70 (MQ=255) aaCGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGAt > 1:138097/1‑70 (MQ=255) aaCGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGAt > 1:2898045/1‑70 (MQ=255) aaCGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGAt > 1:3894634/1‑70 (MQ=255) aaCGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATg > 1:3862813/1‑71 (MQ=255) cGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCg < 1:375722/71‑1 (MQ=255) gCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGt < 1:1763059/71‑1 (MQ=255) gCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGt < 1:1716479/71‑1 (MQ=255) gCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGt < 1:1774647/71‑1 (MQ=255) gCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGt < 1:3961064/71‑1 (MQ=255) ttcttTTTCAATTACCGgtgtgtcatctgtattcagta > 1:1709466‑M2/2‑17 (MQ=255) ttcttTTTCAATTACCGgtgtgtcatctgtattcagta > 1:2928702‑M2/2‑17 (MQ=255) tctTTTTCAATTACCGgtgtgtcatctgtattcagta < 1:378878‑M2/37‑22 (MQ=255) tctTTTTCAATTACCGgtgtgtcatctgtattcagta > 1:2473875‑M2/1‑16 (MQ=255) tctTTTTCAATTACCGgtgtgtcatctgtattcagta < 1:2163955‑M2/37‑22 (MQ=255) tctTTTTCAATTACCGgtgtgtcatctgtattcagta < 1:1590322‑M2/37‑22 (MQ=255) tctTTTTCAATTACCGgtgtgtcatctgtattcagta < 1:2920206‑M2/37‑22 (MQ=255) tctTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGttt > 1:869037/1‑71 (MQ=255) ctTTTTCAATTACCGgtgtgtcatctgtattcagtac > 1:1445550‑M2/1‑15 (MQ=255) ctTTTTCAATTACCGgtgtgtcatctgtattcagtac > 1:1231206‑M2/1‑15 (MQ=255) ctTTTTCAATTACCGgtgtgtcatctgtattcagtac > 1:3946086‑M2/1‑15 (MQ=255) ctTTTTCAATTACCGgtgtgtcatctgtattcagtac > 1:2887637‑M2/1‑15 (MQ=255) ctTTTTCAATTACCGgtgtgtcatctgtattcagtac > 1:3024537‑M2/1‑15 (MQ=255) ctTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGtttt > 1:3026120/1‑61 (MQ=255) ttttCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGcc > 1:4252897/1‑71 (MQ=255) ttttCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGcc > 1:1894822/1‑71 (MQ=255) ttttCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGcc > 1:908728/1‑71 (MQ=255) tttCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGa > 1:1528700/1‑60 (MQ=255) tttCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGa > 1:3648987/1‑60 (MQ=255) tttCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGc < 1:1738383/69‑1 (MQ=255) tttCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGc < 1:1132191/69‑1 (MQ=255) ttCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGc < 1:401419/68‑1 (MQ=255) tCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCa > 1:661089/1‑43 (MQ=255) tCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCa > 1:1475440/1‑43 (MQ=255) tCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCa > 1:1466874/1‑43 (MQ=255) tCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCa > 1:292125/1‑43 (MQ=255) cAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCg > 1:2678475/1‑61 (MQ=255) | ATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGCC > minE/1178938‑1179069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |