| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,240,151 | A→G | 60.0% | L27P (CTG→CCG) | zwf ← | glucose‑6‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,240,151 | 0 | A | G | 60.0% | 27.6 / 44.3 | 40 | L27P (CTG→CCG) | zwf | glucose‑6‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (9/7); new base G (9/15); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
GCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCT > minE/1240090‑1240213 | gCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTac > 1:2664964/1‑71 (MQ=255) aTAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCGGCaa < 1:609451/62‑1 (MQ=255) cGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCGGCAATTTACGACGCGCaa < 1:3361263/70‑1 (MQ=255) cGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCGGCAATTTACGACGCGCAAg < 1:3177677/71‑1 (MQ=255) tgtCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCg > 1:201824/1‑71 (MQ=255) cGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCtt < 1:484222/71‑1 (MQ=255) cGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCtt < 1:1321015/71‑1 (MQ=255) ttGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTcgc < 1:1249525/71‑1 (MQ=255) cttaCCGGCTGTTTCCAGTTGATACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGa < 1:423707/68‑1 (MQ=255) cTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGa < 1:3322385/71‑1 (MQ=255) aCCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGaa < 1:1968104/69‑1 (MQ=255) ccGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAAt < 1:372472/71‑1 (MQ=255) ccGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAAt < 1:3660781/71‑1 (MQ=255) cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTacgacg > 1:1374192/1‑43 (MQ=255) cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGAcgc > 1:265230/1‑44 (MQ=255) cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGAcgc > 1:1273810/1‑44 (MQ=255) cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGAcgc > 1:3034050/1‑44 (MQ=255) cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGAcgc > 1:3397137/1‑44 (MQ=255) cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGAcgc > 1:3444556/1‑44 (MQ=255) cTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGAcc < 1:671866/71‑1 (MQ=255) ttGATACAGGGAAGGCAGCAATTTACGACGCGCAAg < 1:1358523/36‑1 (MQ=255) tGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGc > 1:4172802/1‑71 (MQ=255) gATACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGcc > 1:1825779/1‑71 (MQ=255) gATACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGcc > 1:1162994/1‑71 (MQ=255) gATACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGc > 1:4209281/1‑70 (MQ=255) gATACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGc > 1:4159573/1‑70 (MQ=255) tACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCtt < 1:492671/40‑1 (MQ=255) tACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCtt < 1:4239072/40‑1 (MQ=255) tACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCtt < 1:426231/40‑1 (MQ=255) tACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCtt < 1:1114376/40‑1 (MQ=255) tACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCtt < 1:684492/40‑1 (MQ=255) tACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCtt < 1:3667473/40‑1 (MQ=255) tACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCtt < 1:3525574/40‑1 (MQ=255) tACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCtt < 1:2685120/40‑1 (MQ=255) tACAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCtt < 1:2149425/40‑1 (MQ=255) cAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAg > 1:3976967/1‑57 (MQ=255) cAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAg > 1:3557212/1‑57 (MQ=255) cAGGGAAGGCGGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAg > 1:1835577/1‑57 (MQ=255) cAGGGAAGGCGGCAATTTACGACGCGCAACGTCGCCTTTCGCGCCGAAAATGa > 1:4023795/1‑53 (MQ=255) gggAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCt > 1:456138/1‑71 (MQ=255) gcagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTca < 1:30645/53‑1 (MQ=255) gcagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTca < 1:291051/53‑1 (MQ=255) gcagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTca < 1:109832/53‑1 (MQ=255) cagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTca < 1:3632872/52‑1 (MQ=255) cagcaATTTACGACGCACAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTca < 1:795416/52‑1 (MQ=255) | GCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCT > minE/1240090‑1240213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |