| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,249,224 | C→T | 45.2% | D17N (GAT→AAT) | ruvB ← | ATP‑dependent DNA helicase, component of RuvABC resolvasome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,249,224 | 0 | C | T | 45.2% | 24.9 / 99.3 | 73 | D17N (GAT→AAT) | ruvB | ATP‑dependent DNA helicase, component of RuvABC resolvasome |
| Reads supporting (aligned to +/- strand): ref base C (26/14); new base T (12/21); total (38/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
AACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAACGC > minE/1249158‑1249289 | aacgaacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTcc > 1:1146627/1‑71 (MQ=255) aacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCTGCa < 1:703556/71‑1 (MQ=255) aacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCa < 1:830925/71‑1 (MQ=255) acCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCa < 1:4163913/70‑1 (MQ=255) cTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAg < 1:3009676/71‑1 (MQ=255) cTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAg < 1:1289046/71‑1 (MQ=255) cTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAg < 1:1592501/71‑1 (MQ=255) cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:4225050/69‑1 (MQ=255) cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:3170994/69‑1 (MQ=255) cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2105487/69‑1 (MQ=255) gACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCg > 1:2915738/1‑71 (MQ=255) cAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAg > 1:563035/1‑60 (MQ=255) cAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCgg > 1:475323/1‑69 (MQ=255) aaCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:1204116/60‑1 (MQ=255) aaCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg > 1:834757/1‑68 (MQ=255) aaCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg > 1:395449/1‑68 (MQ=255) aaCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg > 1:1249954/1‑68 (MQ=255) aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAg > 1:3181956/1‑70 (MQ=255) aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAg > 1:2299415/1‑70 (MQ=255) cATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTgg > 1:4031286/1‑60 (MQ=255) cATACTATTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCaaa > 1:2387989/1‑56 (MQ=255) aTACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGaaa > 1:3056356/1‑71 (MQ=255) aTACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGaaa < 1:787148/71‑1 (MQ=255) tACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGaaa > 1:981451/1‑70 (MQ=255) tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGaaa > 1:3361186/1‑67 (MQ=255) tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:1776411/1‑68 (MQ=255) tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:2614867/1‑68 (MQ=255) tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGG‑‑GAAATCAGAc > 1:3568926/1‑71 (MQ=255) tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGCCAGAAAt > 1:377011/1‑68 (MQ=255) ctTCCAGTAATTTGGGGCGAATGGCGAGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:1558141/1‑71 (MQ=255) ttCCAGTAATTTGGTGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGt < 1:1266732/54‑1 (MQ=255) ttCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGt < 1:3579695/54‑1 (MQ=255) ttCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGt < 1:3635833/54‑1 (MQ=255) ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:20616/1‑42 (MQ=255) ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACgg > 1:1246490/1‑71 (MQ=255) ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACgg > 1:1256308/1‑71 (MQ=255) tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:578143/41‑1 (MQ=255) tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:3448699/41‑1 (MQ=255) tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:25568/41‑1 (MQ=255) tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:401346/41‑1 (MQ=255) tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:1669431/41‑1 (MQ=255) tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:2436797/41‑1 (MQ=255) tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:2307278/41‑1 (MQ=255) tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:4113650/41‑1 (MQ=255) aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:321380/1‑36 (MQ=255) aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:1639508/1‑36 (MQ=255) aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:190319/1‑36 (MQ=255) aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:1920083/1‑36 (MQ=255) aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:2481764/1‑36 (MQ=255) aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:2774765/1‑36 (MQ=255) tttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:3520759/1‑61 (MQ=255) tttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:1372634/1‑61 (MQ=255) tttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:2135945/1‑61 (MQ=255) tttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:2501970/1‑61 (MQ=255) ttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCtt > 1:3395351/1‑70 (MQ=255) cGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAatcat > 1:3579350/1‑71 (MQ=255) cGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAatca > 1:1560675/1‑70 (MQ=255) aTGGCGCGATCTGCTACATTTTCCGGCAAAGTGGt < 1:1040488/35‑1 (MQ=255) aTGGCGCGATCTGCTACATTTTCCGGCAAAGTGGt < 1:954882/35‑1 (MQ=255) aTGGCGCGATCTGCTACATTTTCCGGCAAAGTGGt < 1:959857/35‑1 (MQ=255) aTGGCGCGATCTGCTACATTTTCCGGCAAAGTGGt < 1:1565779/35‑1 (MQ=255) tGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGaa < 1:1396016/44‑1 (MQ=255) tGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCtt < 1:116473/71‑1 (MQ=255) ggCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCtt > 1:4186057/1‑59 (MQ=255) cgATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCt > 1:954661/1‑71 (MQ=255) tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:1552159/68‑1 (MQ=255) tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:3931981/68‑1 (MQ=255) tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:3616069/68‑1 (MQ=255) tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:3104118/68‑1 (MQ=255) tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:152259/68‑1 (MQ=255) tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:3497568/68‑1 (MQ=255) cTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAAcg > 1:2800280/1‑71 (MQ=255) cATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAAcgc < 1:1568004/69‑1 (MQ=255) | AACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAACGC > minE/1249158‑1249289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |