| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,257,376 | T→C | 54.1% | F7L (TTT→CTT) | yecP → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,257,376 | 0 | T | C | 54.1% | 19.9 / 101.9 | 74 | F7L (TTT→CTT) | yecP | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (14/20); new base C (25/15); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01 | |||||||||||
TCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACT > minE/1257306‑1257445 | tCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACt > 1:1202377/1‑71 (MQ=255)tCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACt > 1:2536861/1‑71 (MQ=255) aGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACcttt < 1:3600196/71‑1 (MQ=255) aGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACcttt < 1:2238648/71‑1 (MQ=255) cTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtc > 1:3671387/1‑71 (MQ=255) cTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtc > 1:1168638/1‑71 (MQ=255) ttAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATtctc > 1:744925/1‑71 (MQ=255) tAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATtct < 1:2603548/69‑1 (MQ=255) tAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATtct < 1:2598433/69‑1 (MQ=255) tAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATtct < 1:1633317/69‑1 (MQ=255) aaCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTctct < 1:2583366/70‑1 (MQ=255) aCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGa < 1:1039540/71‑1 (MQ=255) aCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGa > 1:4060543/1‑71 (MQ=255) aCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGa > 1:716716/1‑71 (MQ=255) ttGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTg > 1:2607080/1‑71 (MQ=255) ttGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTg > 1:3585499/1‑71 (MQ=255) ggTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGc > 1:3740382/1‑70 (MQ=255) ggTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGc > 1:3353000/1‑70 (MQ=255) tggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAat > 1:1630079/1‑70 (MQ=255) tgtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatca > 1:1434989/2‑71 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTTGTAACCTTTAtt < 1:654178/52‑1 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt > 1:3720466/1‑51 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt > 1:3351825/1‑51 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatta > 1:880334/1‑69 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTAtt < 1:3908972/52‑1 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTAtt < 1:254244/52‑1 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTAtt < 1:1261536/52‑1 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTAtt < 1:3342053/52‑1 (MQ=255) gCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCtt > 1:3429216/1‑71 (MQ=255) gCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCtt > 1:578620/1‑71 (MQ=255) cATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1870959/71‑1 (MQ=255) ttAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTctct < 1:1197695/49‑1 (MQ=255) ttAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTctct < 1:1095295/49‑1 (MQ=255) aaaaGCAGAGGATGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:3534894/1‑71 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGa > 1:1105967/1‑49 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatcatc > 1:2728450/1‑64 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcact > 1:2717238/1‑70 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:1561596/1‑71 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:750950/1‑71 (MQ=255) aaGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAatc > 1:1547454/1‑59 (MQ=255) gCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTa > 1:668629/1‑37 (MQ=255) gCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTa > 1:3262207/1‑37 (MQ=255) agGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATAATCTTTCAC‑CTGGCTCGa < 1:2287760/71‑1 (MQ=255) ggACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa < 1:26888/71‑1 (MQ=255) gACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa > 1:577953/1‑70 (MQ=255) gACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa > 1:3547378/1‑70 (MQ=255) gACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa > 1:2004623/1‑70 (MQ=255) aCGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGaaa > 1:3436205/1‑71 (MQ=255) aCGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGaaa > 1:913113/1‑71 (MQ=255) aCGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGaa > 1:2917145/1‑70 (MQ=255) cGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAAc < 1:518500/71‑1 (MQ=255) tGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGaaa > 1:3326435/1‑67 (MQ=255) tGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCt > 1:1341767/1‑71 (MQ=255) tGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCt > 1:2510383/1‑71 (MQ=255) cATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCt < 1:1040815/69‑1 (MQ=255) cATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGc > 1:2631216/1‑71 (MQ=255) aTGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCtt < 1:397891/48‑1 (MQ=255) tGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGaaa > 1:323844/1‑63 (MQ=255) tGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGaaa > 1:2708821/1‑63 (MQ=255) gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:863111/71‑1 (MQ=255) gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:2149486/71‑1 (MQ=255) tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:3739777/40‑1 (MQ=255) tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1971200/40‑1 (MQ=255) tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:1111704/68‑1 (MQ=255) tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:350423/68‑1 (MQ=255) tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:2540413/68‑1 (MQ=255) gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa < 1:2006508/49‑1 (MQ=255) gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:4103386/71‑1 (MQ=255) gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:3016333/71‑1 (MQ=255) gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:222187/71‑1 (MQ=255) gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:3089098/71‑1 (MQ=255) gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:80495/71‑1 (MQ=255) tAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcg < 1:395609/59‑1 (MQ=255) tAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcg < 1:3456812/59‑1 (MQ=255) tAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTa < 1:3880701/71‑1 (MQ=255) tAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGc > 1:405844/1‑69 (MQ=255) aCTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAAc > 1:190212/1‑71 (MQ=255) cTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACt < 1:2615238/71‑1 (MQ=255) | TCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACT > minE/1257306‑1257445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |