| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,291,126 | G→A | 66.3% | intergenic (+241/+212) | yeeN → / ← asnW | conserved hypothetical protein/tRNA‑Asn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,291,126 | 0 | G | A | 66.3% | 96.6 / 84.6 | 86 | intergenic (+241/+212) | yeeN/asnW | conserved hypothetical protein/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base G (19/10); new base A (40/17); total (59/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAAT > minE/1291056‑1291191 | aaTTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTg < 1:1265411/71‑1 (MQ=255)aaTTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTg < 1:1475287/71‑1 (MQ=255) ttACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTa > 1:1547676/1‑71 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATGCCGCATAAGGCATTTTTCTCTTTATCTTTATACg > 1:408330/1‑71 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACg > 1:667276/1‑71 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACg > 1:2694441/1‑71 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACg > 1:2135621/1‑71 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACg > 1:1965672/1‑71 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACg > 1:1823299/1‑71 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACg > 1:495848/1‑71 (MQ=255) aTAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTa > 1:1317389/1‑65 (MQ=255) aTAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTa > 1:2382735/1‑65 (MQ=255) aTAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTAc > 1:3643790/1‑71 (MQ=255) aTAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTAc > 1:748699/1‑71 (MQ=255) aTAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTAc > 1:1974617/1‑71 (MQ=255) tCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATg > 1:3244514/1‑71 (MQ=255) tCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATg > 1:975913/1‑71 (MQ=255) aaaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTg < 1:792748/50‑1 (MQ=255) aaaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTg < 1:3634673/50‑1 (MQ=255) aaaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAAc < 1:1493524/68‑1 (MQ=255) aaaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACa > 1:1305607/1‑69 (MQ=255) aaaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACa > 1:1300628/1‑69 (MQ=255) aaaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACa > 1:3846695/1‑69 (MQ=255) gACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGtt < 1:1773113/71‑1 (MQ=255) cgATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAAc < 1:1123017/61‑1 (MQ=255) aGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTtata > 1:910537/1‑41 (MQ=255) aGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTtata > 1:62312/1‑41 (MQ=255) aGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCt > 1:2315080/1‑71 (MQ=255) aGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCt > 1:2417961/1‑71 (MQ=255) aGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCt > 1:964480/1‑71 (MQ=255) gCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGaa < 1:4127337/55‑1 (MQ=255) gCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGaa < 1:4049130/55‑1 (MQ=255) gCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGaa < 1:3956810/55‑1 (MQ=255) gCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGaa < 1:118097/55‑1 (MQ=255) gCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACa > 1:4028246/1‑57 (MQ=255) gCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACag < 1:570976/58‑1 (MQ=255) gACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACa > 1:2468263/1‑55 (MQ=255) aTCCCGCATAAGGCATTTTTCTCTTTATCTTtata < 1:1338151/35‑1 (MQ=255) aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTa > 1:2251041/1‑35 (MQ=255) aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTa > 1:3149422/1‑35 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACaga > 1:693382/1‑51 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGa > 1:1651620/1‑59 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGa > 1:4162963/1‑59 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGa > 1:1549680/1‑59 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATc < 1:3036371/70‑1 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATc < 1:3586956/70‑1 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATc < 1:2425107/70‑1 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCg > 1:465579/1‑71 (MQ=255) cGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACag < 1:2997257/49‑1 (MQ=255) cGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:782466/71‑1 (MQ=255) cGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:306034/71‑1 (MQ=255) gCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt > 1:2315708/1‑70 (MQ=255) cATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACa < 1:4071059/46‑1 (MQ=255) tAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:3576574/67‑1 (MQ=255) tAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:1464529/67‑1 (MQ=255) aaGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATg < 1:888699/70‑1 (MQ=255) aGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:1002186/1‑67 (MQ=255) aGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:50942/1‑67 (MQ=255) aGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTATGACCTTGCGAATc > 1:567466/1‑63 (MQ=255) ggCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACaga > 1:2562156/1‑43 (MQ=255) ggCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACaga > 1:814322/1‑43 (MQ=255) ggCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACaga > 1:1852100/1‑43 (MQ=255) ggCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACaga > 1:1314578/1‑43 (MQ=255) tttttCTCTTTATCTTTATACTGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg < 1:813682/71‑1 (MQ=255) tttttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg < 1:1107682/71‑1 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:3289075/1‑71 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:3284273/1‑71 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGa > 1:2320189/1‑45 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGa > 1:3786699/1‑45 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGa > 1:3782755/1‑45 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGa > 1:335906/1‑45 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:2831977/1‑71 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:3783608/1‑71 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:450471/1‑71 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:1116773/1‑71 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg > 1:3270754/1‑70 (MQ=255) ttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:273033/1‑70 (MQ=255) ctTTATCTTTGTTCGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGt < 1:3293675/62‑1 (MQ=255) ttATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg < 1:428406/64‑1 (MQ=255) ttATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg < 1:3148782/64‑1 (MQ=255) ttATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg < 1:344838/64‑1 (MQ=255) tATCTTTGTACGGTACTTCATGGAACAGAGttttt > 1:1064843/1‑35 (MQ=255) tATCTTTATACGGTACTTCATGGAACAGAGTTTTTGAc > 1:638229/1‑38 (MQ=255) tATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGAc > 1:3212911/1‑70 (MQ=255) aTCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACaa > 1:1280867/1‑71 (MQ=255) tCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg < 1:3957600/59‑1 (MQ=255) tCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACaa < 1:2180286/70‑1 (MQ=255) tCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACaa < 1:188226/70‑1 (MQ=255) tCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAAt > 1:3834142/1‑71 (MQ=255) tCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAAt > 1:3432385/1‑71 (MQ=255) tCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAAt > 1:3342984/1‑71 (MQ=255) tCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAAt > 1:2192742/1‑71 (MQ=255) tCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAAt > 1:1343734/1‑71 (MQ=255) | AATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAAT > minE/1291056‑1291191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |