| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,408,722 | G→A | 35.0% | A331T (GCG→ACG) | yejF → | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,408,722 | 0 | G | A | 35.0% | 45.9 / 34.3 | 40 | A331T (GCG→ACG) | yejF | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (10/16); new base A (8/6); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAA > minE/1408655‑1408788 | gCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGacgc < 1:2800864/71‑1 (MQ=255) cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGACgctgct > 1:4182283/1‑71 (MQ=255) gggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGAc > 1:3648874/1‑71 (MQ=255) acTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAAtt < 1:2639982/71‑1 (MQ=255) cTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATtc < 1:4025108/71‑1 (MQ=255) gggTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATtct > 1:467470/1‑70 (MQ=255) ggggCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATtc < 1:2161224/60‑1 (MQ=255) ggCGAGTCCGGTACCGGGAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGcat > 1:1034599/1‑71 (MQ=255) ggCGAGTCCGGGTCCGGGAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGcat > 1:3651468/1‑71 (MQ=255) cGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTg < 1:907432/71‑1 (MQ=255) cGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTg < 1:1313264/71‑1 (MQ=255) ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGt > 1:2321671/1‑71 (MQ=255) ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGt > 1:2465670/1‑71 (MQ=255) ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGt > 1:787297/1‑71 (MQ=255) ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGt > 1:496580/1‑71 (MQ=255) gggAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcatc < 1:387004/57‑1 (MQ=255) gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATtc > 1:3739460/1‑42 (MQ=255) gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcatcat > 1:1759228/1‑57 (MQ=255) gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcatcat > 1:1709955/1‑57 (MQ=255) gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcatcat > 1:435666/1‑57 (MQ=255) gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGc < 1:25716/71‑1 (MQ=255) gAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGc > 1:1024645/1‑71 (MQ=255) aaaaGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGcc < 1:4205840/71‑1 (MQ=255) aaaaGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGcc < 1:830111/71‑1 (MQ=255) acgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa > 1:1011355/1‑56 (MQ=255) acgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa > 1:4029728/1‑56 (MQ=255) acgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa > 1:4251111/1‑56 (MQ=255) acgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa > 1:3807940/1‑56 (MQ=255) cgacgGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGc < 1:3949744/69‑1 (MQ=255) cgacgGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGc < 1:4143515/69‑1 (MQ=255) cgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCaa < 1:1272137/71‑1 (MQ=255) cgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCaa < 1:4033570/71‑1 (MQ=255) gggACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAAtt < 1:2202081/71‑1 (MQ=255) gggACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAACCACTGCAAAAtt < 1:367070/71‑1 (MQ=255) ggACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAAttt < 1:1805829/71‑1 (MQ=255) ggACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAAttt < 1:727758/71‑1 (MQ=255) aCTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:1348707/71‑1 (MQ=255) aCTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:3191514/71‑1 (MQ=255) aCTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:1736644/71‑1 (MQ=255) aCTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:2772017/71‑1 (MQ=255) aCTGGCGCTGATGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:4069688/71‑1 (MQ=255) | GCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAA > minE/1408655‑1408788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |