| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,418,334 | G→A | 66.2% | V95I (GTC→ATC) | narP → | DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,418,334 | 0 | G | A | 66.2% | 73.9 / 67.3 | 68 | V95I (GTC→ATC) | narP | DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| Reads supporting (aligned to +/- strand): ref base G (10/13); new base A (28/17); total (38/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTAT > minE/1418268‑1418397 | gCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCtt > 1:2464433/1‑71 (MQ=255)gCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCtt > 1:2034379/1‑71 (MQ=255) ccTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCttt > 1:4051155/1‑71 (MQ=255) ccTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCttt > 1:2613106/1‑71 (MQ=255) cgcAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCt < 1:3514373/71‑1 (MQ=255) cAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa < 1:1521351/71‑1 (MQ=255) cAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa < 1:1837685/71‑1 (MQ=255) cAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa < 1:3429298/71‑1 (MQ=255) cAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa < 1:2906648/71‑1 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGa > 1:815351/1‑70 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGAc > 1:681350/1‑71 (MQ=255) tGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCTATGCCTCCAGCGATGTCTTTgc < 1:1291870/60‑1 (MQ=255) tGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTgc < 1:612367/60‑1 (MQ=255) tGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTgc < 1:1373835/60‑1 (MQ=255) tGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATa < 1:39716/67‑1 (MQ=255) tGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATa < 1:1361339/67‑1 (MQ=255) aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGgcg > 1:967295/1‑70 (MQ=255) aaaTTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGt < 1:2101787/71‑1 (MQ=255) aaTTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCt < 1:1243079/48‑1 (MQ=255) aaTTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCt < 1:4054441/48‑1 (MQ=255) aaTTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCt < 1:3811547/48‑1 (MQ=255) aaTTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCt < 1:2861269/48‑1 (MQ=255) aaTTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGtt < 1:4178642/71‑1 (MQ=255) attattATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTa > 1:1818617/1‑71 (MQ=255) attattATCCTGACCGTATCCGATGCCTACAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTa > 1:1682649/1‑71 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTAt < 1:1799774/71‑1 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTAt < 1:3612320/71‑1 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTa > 1:2438688/1‑70 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTa > 1:2667875/1‑70 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTAt > 1:3614735/1‑71 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTAt > 1:3764975/1‑71 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTAt > 1:3637691/1‑71 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTACAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTAt > 1:3620620/1‑71 (MQ=255) tattatCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTAt < 1:2777949/70‑1 (MQ=255) tattatCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa > 1:711203/1‑47 (MQ=255) tattatCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa > 1:3863178/1‑47 (MQ=255) tattatCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa > 1:418569/1‑47 (MQ=255) tattatCCTGACCGTATCCGATGACTCCAGCGATATCTTTGCGCTGa > 1:4007153/1‑47 (MQ=39) ttatCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa > 1:1914448/1‑45 (MQ=255) ttatCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa > 1:372287/1‑45 (MQ=255) tCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGa < 1:1842521/42‑1 (MQ=255) ccTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTg > 1:279848/1‑70 (MQ=255) aCCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGa > 1:2527629/1‑37 (MQ=255) aCCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGa > 1:2022970/1‑37 (MQ=255) aCCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGa > 1:4070007/1‑37 (MQ=255) aCCGTATCCGATGCCTCCAGCGATATCTTTTCGCTGATAGACGCAGGCGCAGACg > 1:3960116/1‑55 (MQ=39) aCCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaaa < 1:2384924/69‑1 (MQ=255) aCCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGa > 1:2327021/1‑71 (MQ=255) cGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACa < 1:3015172/71‑1 (MQ=255) gTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGtt < 1:2934232/55‑1 (MQ=255) gTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGtt < 1:4219478/55‑1 (MQ=255) gTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGtt < 1:3326493/55‑1 (MQ=255) tATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGc < 1:1637693/71‑1 (MQ=255) tATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGtt < 1:2111969/54‑1 (MQ=255) tCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGa > 1:1873940/1‑71 (MQ=255) ccGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGa < 1:828771/60‑1 (MQ=255) cGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:2427108/1‑71 (MQ=255) gATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaaa > 1:2758204/1‑60 (MQ=255) gATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaaa > 1:702211/1‑60 (MQ=255) gATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaaa > 1:3026909/1‑60 (MQ=255) gATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaaa > 1:2900662/1‑60 (MQ=255) gCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAccc > 1:3816148/1‑68 (MQ=255) cTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAg < 1:32207/71‑1 (MQ=255) ccAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGtt > 1:210840/1‑49 (MQ=255) cAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTg > 1:498073/1‑46 (MQ=255) cAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTg > 1:1964232/1‑46 (MQ=255) cAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTAt > 1:3079359/1‑71 (MQ=255) cAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTAt < 1:4072277/71‑1 (MQ=255) | GCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTAT > minE/1418268‑1418397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |