| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,475,665 | A→T | 48.6% | T244S (ACC→TCC) | nrdB → | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,475,665 | 0 | A | T | 48.6% | ‑5.9 / 103.4 | 70 | T244S (ACC→TCC) | nrdB | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base A (27/9); new base T (18/16); total (45/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01 | |||||||||||
GCGAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAAT > minE/1475600‑1475729 | gcgAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGc < 1:2520440/71‑1 (MQ=255)gcgAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGc < 1:2700037/71‑1 (MQ=255) gATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAg < 1:277064/70‑1 (MQ=255) aTGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCa < 1:415052/71‑1 (MQ=255) aTGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCa < 1:4196738/71‑1 (MQ=255) aTGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCa < 1:3090789/71‑1 (MQ=255) gCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCt > 1:2192071/1‑70 (MQ=255) cGCCGAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTTTCCGGCACCCAGc < 1:3071682/59‑1 (MQ=255) cGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGc < 1:1201954/59‑1 (MQ=255) gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCCGCata > 1:2737361/1‑61 (MQ=255) gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCata > 1:3540688/1‑61 (MQ=255) gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCata > 1:2479399/1‑61 (MQ=255) gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCata > 1:369456/1‑61 (MQ=255) gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATct > 1:2227613/1‑71 (MQ=255) ccAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATctg > 1:351621/1‑71 (MQ=255) ccAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATctg > 1:364272/1‑71 (MQ=255) aaaaTTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATctgct < 1:956886/71‑1 (MQ=255) ttattCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTgcgc < 1:2074900/71‑1 (MQ=255) ttCGCCTGATTGCCTGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAgc < 1:1520514/71‑1 (MQ=255) ttCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg < 1:2620987/70‑1 (MQ=255) cGCCTGATTGCCCGCGCCGAAGCCCTGCACCTGTCCGGCCCCCAGCATATGCTGAATCTGCTGCGCAg > 1:1852713/1‑68 (MQ=255) cGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg > 1:1526752/1‑68 (MQ=255) cGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg > 1:70061/1‑68 (MQ=255) cGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg > 1:1289253/1‑68 (MQ=255) ccTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGc < 1:3936258/37‑1 (MQ=255) tGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTgcgc < 1:2369457/62‑1 (MQ=255) tGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:3310229/1‑71 (MQ=255) tGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:1212557/1‑71 (MQ=255) gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg < 1:3012560/63‑1 (MQ=255) gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:2250382/1‑70 (MQ=255) gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:455992/1‑70 (MQ=255) gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:3875364/1‑70 (MQ=255) gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:2743611/1‑70 (MQ=255) cccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGa < 1:200174/70‑1 (MQ=255) cccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGa < 1:144582/70‑1 (MQ=255) cccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGAt < 1:2978092/71‑1 (MQ=255) cccGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGa < 1:3876784/70‑1 (MQ=255) cgcgACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg < 1:222416/56‑1 (MQ=255) gcgACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:4066903/1‑71 (MQ=255) gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:324433/1‑69 (MQ=255) gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:922770/1‑69 (MQ=255) gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:541663/1‑69 (MQ=255) gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga < 1:1198786/71‑1 (MQ=255) gacgaAGCCCTGCACCTGACCGGCACCCAGCATAAGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTg > 1:2690541/1‑70 (MQ=255) cgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACg > 1:3437178/1‑62 (MQ=255) cgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:2324019/1‑67 (MQ=255) gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:923235/1‑68 (MQ=255) gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:1727885/1‑68 (MQ=255) gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:2170450/1‑68 (MQ=255) aGCCCTGCACCTGTCCGGCA‑CCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGc > 1:492917/1‑71 (MQ=255) cccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:2071647/1‑64 (MQ=255) cccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:2413883/1‑64 (MQ=255) cccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:3816489/1‑64 (MQ=255) cccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTg > 1:2017773/1‑63 (MQ=255) ccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCg > 1:975543/1‑70 (MQ=255) cTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:1839362/71‑1 (MQ=255) cTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:290611/71‑1 (MQ=255) cTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:295652/71‑1 (MQ=255) cTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:3033548/71‑1 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGaaa > 1:1932230/1‑71 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGaaa > 1:119628/1‑71 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGaaa > 1:30341/1‑71 (MQ=255) cACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAAt > 1:2701638/1‑71 (MQ=255) cACCTGTCCGGCAACCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAAt > 1:2737911/1‑71 (MQ=255) cACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa > 1:3289723/1‑50 (MQ=255) cACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa > 1:1225759/1‑50 (MQ=255) cACCTGACCGGCACCCAGCATATGCAGAATCTGCTGCGCAGCGGCGCgg > 1:1281017/1‑49 (MQ=255) tGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTgaga > 1:2846701/1‑57 (MQ=255) gACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTg > 1:1899434/1‑53 (MQ=255) gACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTg > 1:423402/1‑53 (MQ=255) | GCGAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAAT > minE/1475600‑1475729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |