| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 204,556 | A→G | 100% | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 204,556 | 0 | A | G | 100.0% | 279.5 / NA | 91 | intergenic (+156/‑28) | alaV/rrlH | tRNA‑Ala/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (61/30); total (61/30) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT > minE/204495‑204623 | ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa > 1:2880215/1‑69 (MQ=255)ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa > 1:2220965/1‑69 (MQ=255)ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg > 1:2775875/1‑70 (MQ=255)ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg > 1:2805790/1‑70 (MQ=255)ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg > 1:378510/1‑70 (MQ=255)ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg > 1:3822318/1‑70 (MQ=255)ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATATTCGCAACACGATGATGGATCGCaa > 1:984471/1‑69 (MQ=11) acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa > 1:3273451/1‑70 (MQ=255) acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt > 1:2547118/1‑71 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:1926888/1‑71 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:3324080/1‑71 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:1946345/1‑71 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:1197808/1‑71 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc < 1:3111631/69‑1 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg < 1:473423/71‑1 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg < 1:1962292/71‑1 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:2273410/3‑71 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:708231/3‑71 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:3355898/3‑71 (MQ=255) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:1248901/2‑71 (MQ=19) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:1912488/2‑71 (MQ=19) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:2893750/2‑71 (MQ=19) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:4018379/2‑70 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa > 1:2033613/1‑56 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa > 1:4038565/1‑56 (MQ=255) tctgttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt < 1:2968727/66‑1 (MQ=255) aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg > 1:1679661/1‑71 (MQ=21) ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt < 1:2743497/71‑1 (MQ=21) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:2675555/47‑1 (MQ=255) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa > 1:3736816/1‑51 (MQ=255) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:3318539/1‑69 (MQ=25) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:1378375/46‑1 (MQ=255) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:2366831/46‑1 (MQ=255) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:2420095/46‑1 (MQ=255) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:2624866/46‑1 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:2287976/1‑59 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:2403023/1‑59 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:1719856/1‑59 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:304664/1‑59 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:1603927/1‑59 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2045748/70‑1 (MQ=25) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2986095/70‑1 (MQ=25) gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa < 1:451693/61‑1 (MQ=25) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:3357789/42‑1 (MQ=255) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt > 1:992964/1‑70 (MQ=25) tctctcTAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa < 1:3165734/56‑1 (MQ=11) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt < 1:1186337/45‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt < 1:4215737/45‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa < 1:865400/56‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa < 1:3294412/56‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAg < 1:3661268/71‑1 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc > 1:1037908/1‑45 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc > 1:686401/1‑45 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc > 1:4024608/1‑45 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc > 1:1713337/1‑45 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt < 1:65449/52‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:806148/1‑71 (MQ=21) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg > 1:2820832/1‑44 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg > 1:1413221/1‑44 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg > 1:211628/1‑44 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg > 1:3097567/1‑44 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:3554538/1‑71 (MQ=21) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:3630088/1‑71 (MQ=21) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:1873809/1‑71 (MQ=21) tcAAATATTCGCAACACGATGATGGATCGCAAGAAACATCTTCg > 1:7732/1‑44 (MQ=11) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg > 1:1706285/1‑45 (MQ=255) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:1677986/1‑70 (MQ=25) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:1690351/1‑70 (MQ=25) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:3953784/1‑70 (MQ=25) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:3243030/1‑70 (MQ=25) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:2106141/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca < 1:931428/71‑1 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:2550240/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca < 1:4076860/71‑1 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca < 1:3956767/71‑1 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:384727/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3832998/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:1727100/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3693372/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca < 1:2374175/71‑1 (MQ=21) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac > 1:1512971/1‑71 (MQ=21) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca < 1:3746661/70‑1 (MQ=25) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca < 1:2587686/70‑1 (MQ=25) ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg > 1:2171487/1‑71 (MQ=21) ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg > 1:270621/1‑70 (MQ=25) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:1727360/70‑1 (MQ=25) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:2853537/70‑1 (MQ=25) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc > 1:906362/1‑71 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:1920951/1‑71 (MQ=21) gatgatGGATCGCAAGAAACATCTTCGTGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg < 1:591390/71‑1 (MQ=255) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg > 1:1568243/1‑71 (MQ=21) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGt < 1:2494322/71‑1 (MQ=21) | TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT > minE/204495‑204623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |