| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,511,807 | G→A | 53.7% | E359K (GAG→AAG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,511,807 | 0 | G | A | 53.7% | 11.2 / 117.4 | 80 | E359K (GAG→AAG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (17/20); new base A (21/22); total (38/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
CTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAC > minE/1511740‑1511871 | cTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCgaga < 1:3652376/71‑1 (MQ=255) ccTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAg > 1:4034694/1‑71 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAgg < 1:2172342/71‑1 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAgg < 1:3367704/71‑1 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAgg < 1:3803504/71‑1 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAg > 1:1376958/1‑70 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAg > 1:820249/1‑70 (MQ=255) tctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTGTCGAGAAAGTTCAgg < 1:19612/70‑1 (MQ=255) ctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGaaa > 1:622430/1‑62 (MQ=255) ctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGaaa > 1:3388283/1‑62 (MQ=255) ctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGaaa > 1:2890418/1‑62 (MQ=255) cTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGc > 1:1896770/1‑71 (MQ=255) gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTg > 1:2233452/1‑68 (MQ=255) cAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:1373885/69‑1 (MQ=255) cAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:2703576/69‑1 (MQ=255) cAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTaa > 1:833506/1‑71 (MQ=255) cAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTaa > 1:2844142/1‑71 (MQ=255) agagCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAAc > 1:2916865/1‑71 (MQ=255) agagCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAAc > 1:903960/1‑71 (MQ=255) agagCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAAc > 1:1132196/1‑71 (MQ=255) tCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTaa > 1:1411270/1‑64 (MQ=255) tCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTaa > 1:207429/1‑64 (MQ=255) cAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATc < 1:1427741/70‑1 (MQ=255) tGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTaa > 1:752295/1‑58 (MQ=255) tGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTaa > 1:2138068/1‑58 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCt < 1:2231928/69‑1 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCt < 1:1088839/69‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCt < 1:968988/51‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCt < 1:2390189/51‑1 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:2194994/1‑51 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:1223039/1‑51 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:603913/1‑51 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:423290/1‑51 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:1404171/1‑51 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:1560706/1‑51 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:2955545/1‑51 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGa > 1:1947914/1‑71 (MQ=255) tCCGGGTGACGATCACGAACGGATCGAGAAAGTTAAGGAATACGTTGCTAACTACATCAACGCTGACTGGa > 1:3468100/1‑71 (MQ=255) ccGGTTGACGATCACGAACGTATCGTGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt > 1:4068236/1‑71 (MQ=255) ccGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt > 1:3168071/1‑71 (MQ=255) ccGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt > 1:1041328/1‑71 (MQ=255) ccGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt > 1:2002819/1‑71 (MQ=255) ccGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt > 1:4167643/1‑71 (MQ=255) ccGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt > 1:677989/1‑71 (MQ=255) cGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATc < 1:2273452/56‑1 (MQ=255) cGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATc < 1:1361593/56‑1 (MQ=255) cGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATc < 1:1192245/56‑1 (MQ=255) cGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATc < 1:3848427/56‑1 (MQ=255) cGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATc < 1:2810687/56‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCg < 1:302794/71‑1 (MQ=255) gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt < 1:2669624/53‑1 (MQ=255) gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt < 1:3857112/53‑1 (MQ=255) gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt < 1:1649213/53‑1 (MQ=255) gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt < 1:3332949/53‑1 (MQ=255) gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt < 1:3294712/53‑1 (MQ=255) gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt < 1:1905945/53‑1 (MQ=255) ttGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATc < 1:2912716/53‑1 (MQ=255) gACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAAt < 1:2966921/70‑1 (MQ=255) gACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAAt < 1:1772494/70‑1 (MQ=255) gACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAAt < 1:4077625/70‑1 (MQ=255) gACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTa > 1:1645181/1‑47 (MQ=255) gACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTa > 1:3046268/1‑47 (MQ=255) gACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTa > 1:2617522/1‑47 (MQ=255) cGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTa < 1:339319/41‑1 (MQ=255) cGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTa < 1:3596178/41‑1 (MQ=255) cGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTa < 1:866404/41‑1 (MQ=255) aTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:2208355/38‑1 (MQ=255) aTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:3532504/38‑1 (MQ=255) aTCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTgg < 1:1122084/59‑1 (MQ=255) tCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:3091683/37‑1 (MQ=255) tCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGa < 1:3445546/71‑1 (MQ=255) tCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGa < 1:3137740/71‑1 (MQ=255) cACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGAc > 1:615479/1‑71 (MQ=255) gAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGc > 1:412603/1‑71 (MQ=255) gAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGc > 1:2398523/1‑71 (MQ=255) cGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGGCTGCCAc < 1:2178859/71‑1 (MQ=255) cGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 1:4159563/71‑1 (MQ=255) cGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 1:2162856/71‑1 (MQ=255) cGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 1:607948/71‑1 (MQ=255) cGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 1:1760231/71‑1 (MQ=255) cGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 1:284307/71‑1 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGa < 1:1583382/50‑1 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGa < 1:222289/50‑1 (MQ=255) | CTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAC > minE/1511740‑1511871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |