| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,523,433 | G→T | 37.5% | A78S (GCC→TCC) | flk → | predicted flagella assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,523,433 | 0 | G | T | 37.5% | 85.9 / 44.8 | 56 | A78S (GCC→TCC) | flk | predicted flagella assembly protein |
| Reads supporting (aligned to +/- strand): ref base G (11/24); new base T (13/8); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
AGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTT > minE/1523379‑1523500 | aGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATa < 1:2663950/67‑1 (MQ=255) gCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTgg < 1:2049751/71‑1 (MQ=255) gCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTgg < 1:146877/71‑1 (MQ=255) cGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGgcgc < 1:1248275/71‑1 (MQ=255) cGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGgcgc < 1:2385471/71‑1 (MQ=255) cGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGgcgc < 1:237852/71‑1 (MQ=255) cGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGgcgc < 1:2876469/71‑1 (MQ=255) gATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGgcgc > 1:3038851/1‑70 (MQ=255) gATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGgcgc > 1:2370732/1‑70 (MQ=255) gATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGgcg > 1:3744079/1‑69 (MQ=255) atatTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCaa > 1:3658241/1‑71 (MQ=255) atatTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCaa > 1:3405459/1‑71 (MQ=255) atatTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCaa > 1:3887787/1‑71 (MQ=255) ggTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCActtcccttcc > 1:3934558/1‑45 (MQ=255) gTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTctg < 1:3488493/71‑1 (MQ=255) gggAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGcc > 1:170173/1‑70 (MQ=255) gggAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCcg > 1:1407626/1‑71 (MQ=255) gggAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCcg > 1:2495548/1‑71 (MQ=255) ggAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATat < 1:45771/47‑2 (MQ=255) ggAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATaa < 1:145828/47‑1 (MQ=255) gaTTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCgcgc > 1:1327276/1‑70 (MQ=255) aTTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGgcgc < 1:2750894/51‑1 (MQ=255) tCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCaa < 1:3208947/69‑1 (MQ=255) tCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCaa < 1:3910153/69‑1 (MQ=255) cGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc < 1:2685977/71‑1 (MQ=255) gctgctTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGc > 1:2537060/1‑57 (MQ=255) gctgctTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc > 1:4123441/1‑70 (MQ=255) gctgctTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc > 1:854088/1‑70 (MQ=255) gctgctTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc > 1:2200756/1‑70 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCaaa > 1:2016134/1‑65 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc > 1:1733709/1‑70 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc < 1:2303773/70‑1 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc < 1:820185/70‑1 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc < 1:2796376/70‑1 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc > 1:4195214/1‑70 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc < 1:401093/70‑1 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc > 1:1635754/1‑70 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc < 1:1703128/70‑1 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc < 1:2010616/70‑1 (MQ=255) gctgctTTCGCGTCACTTCCCTGCCCCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc < 1:1036284/70‑1 (MQ=255) tgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGcc > 1:3351968/1‑69 (MQ=255) tgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCa > 1:336792/1‑70 (MQ=255) tgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCa > 1:1971972/1‑70 (MQ=255) gctTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCAtt > 1:1962845/1‑71 (MQ=255) gctTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCAtt > 1:3672338/1‑71 (MQ=255) ctTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCaa > 1:3937933/1‑42 (MQ=255) ctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCAt < 1:2963735/69‑1 (MQ=255) tttCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCaa < 1:3952684/59‑1 (MQ=255) ttCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCa < 1:1202682/39‑1 (MQ=255) tCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCa < 1:2405694/38‑1 (MQ=255) cttcccttccGCTGAGCATAATCTGGCGCAACGTctgc > 1:2933767/1‑38 (MQ=255) cttcccttccGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTc > 1:3549043/1‑60 (MQ=255) cttcccttccGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGc > 1:3165408/1‑71 (MQ=255) cttcccttccGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGc > 1:2109673/1‑71 (MQ=255) cTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGc < 1:1949304/71‑1 (MQ=255) ttCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCt < 1:3992767/60‑1 (MQ=255) ttCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCt < 1:1219196/60‑1 (MQ=255) ttCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCt < 1:2758799/60‑1 (MQ=255) ttCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCt < 1:232805/60‑1 (MQ=255) ccTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCtttt < 1:4158430/71‑1 (MQ=255) ccTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCtttt < 1:451379/71‑1 (MQ=255) | AGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTT > minE/1523379‑1523500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |