| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,533,542 | G→A | 41.5% | P51S (CCT→TCT) | prmB ← | N5‑glutamine methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,533,542 | 0 | G | A | 41.5% | 44.2 / 75.7 | 65 | P51S (CCT→TCT) | prmB | N5‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (13/25); new base A (19/8); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTG > minE/1533474‑1533612 | tttttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc < 1:67682/70‑1 (MQ=255) tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCaa > 1:3889941/1‑70 (MQ=255) gCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACAcc < 1:2377066/70‑1 (MQ=255) gTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGt < 1:383872/69‑1 (MQ=255) gTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTAc > 1:2801779/1‑71 (MQ=255) aCGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCa < 1:3291962/59‑1 (MQ=255) gcgcgGTGCGCATATCTTCCGGAATATCCAGCGGCTGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCa > 1:3829431/1‑71 (MQ=255) gcgcgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCa > 1:1038266/1‑71 (MQ=255) ggTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACaa > 1:3371358/1‑48 (MQ=255) ggTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATcc > 1:3957400/1‑70 (MQ=255) gcgcATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACg > 1:510103/1‑71 (MQ=255) cATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGt < 1:1939827/71‑1 (MQ=255) tatCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGTCtt < 1:456124/57‑1 (MQ=255) tatCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCtt < 1:435640/57‑1 (MQ=255) tatCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCtt < 1:2585017/57‑1 (MQ=255) tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTa > 1:920133/1‑49 (MQ=255) tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTa > 1:3477481/1‑49 (MQ=255) tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTa > 1:3051525/1‑49 (MQ=255) tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTAt < 1:3143975/70‑1 (MQ=255) tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTAt < 1:2186337/70‑1 (MQ=255) gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:2211531/45‑1 (MQ=255) gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:3970257/45‑1 (MQ=255) gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:1388211/45‑1 (MQ=255) gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:3641335/45‑1 (MQ=255) gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:3464573/45‑1 (MQ=255) gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:3288824/45‑1 (MQ=255) gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:103013/45‑1 (MQ=255) tCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc > 1:3970443/1‑71 (MQ=255) tCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc > 1:261908/1‑71 (MQ=255) tCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc > 1:2886513/1‑71 (MQ=255) ccAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:3549339/1‑71 (MQ=255) ccAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:1149045/1‑71 (MQ=255) ccAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:1292494/1‑71 (MQ=255) ccAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:345584/1‑71 (MQ=255) cAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:2623683/1‑70 (MQ=255) cAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:1136796/71‑1 (MQ=255) gCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc > 1:4142049/1‑71 (MQ=255) ggCAGTTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:3999745/71‑1 (MQ=255) ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc > 1:1526894/1‑69 (MQ=255) ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc > 1:3651671/1‑69 (MQ=255) ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:913603/71‑1 (MQ=255) ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:884616/71‑1 (MQ=255) ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:1695278/71‑1 (MQ=255) ggCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:2552986/71‑1 (MQ=255) gCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATACCACGGGTTATCGGTACCGTGACCGTAc > 1:2316661/1‑68 (MQ=255) cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:1251016/65‑1 (MQ=255) cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:1403587/65‑1 (MQ=255) cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:4216081/65‑1 (MQ=255) cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:3412275/65‑1 (MQ=255) cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:933688/65‑1 (MQ=255) cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa < 1:1976979/71‑1 (MQ=255) aGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa < 1:4081005/70‑1 (MQ=255) aGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:966166/64‑1 (MQ=255) agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:2838335/1‑71 (MQ=255) agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:3559460/1‑71 (MQ=255) agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAtt > 1:3579232/1‑70 (MQ=255) agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATAGGTACCGTGACCGTACCAGATAttt > 1:2512050/1‑71 (MQ=255) agagCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:2123009/1‑71 (MQ=255) gagCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt < 1:1918966/70‑1 (MQ=255) gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:2043189/1‑40 (MQ=255) gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:2665560/1‑40 (MQ=255) gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:2737736/1‑40 (MQ=255) gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:1790525/1‑40 (MQ=255) gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:3265259/1‑40 (MQ=255) cGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt < 1:2735510/67‑1 (MQ=255) ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGCCCGTACCAGa > 1:911969/1‑58 (MQ=255) ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa > 1:1649849/1‑58 (MQ=255) ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa > 1:1988373/1‑58 (MQ=255) gCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTg < 1:810547/71‑1 (MQ=255) gCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTg < 1:1101596/71‑1 (MQ=255) | TTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTG > minE/1533474‑1533612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |