| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,569,654 | A→T | 11.8% | intergenic (‑329/‑7) | mntH ← / → nupC | manganese/divalent cation transporter/nucleoside (except guanosine) transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,569,654 | 0 | A | T | 11.8% | 289.5 / 28.0 | 110 | intergenic (‑329/‑7) | mntH/nupC | manganese/divalent cation transporter/nucleoside (except guanosine) transporter |
| Reads supporting (aligned to +/- strand): ref base A (52/45); new base T (11/2); total (63/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
CAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTA > minE/1569587‑1569723 | cAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGa > 1:2741563/1‑68 (MQ=255)cAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGa > 1:3551029/1‑68 (MQ=255)cAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGa > 1:3586179/1‑68 (MQ=255) gATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa < 1:2884320/71‑1 (MQ=255) gATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa < 1:3383747/71‑1 (MQ=255) gATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa < 1:184291/71‑1 (MQ=255) gATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa < 1:3783286/71‑1 (MQ=255) aTCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa > 1:1400346/1‑70 (MQ=255) aTCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa > 1:1509855/1‑70 (MQ=255) ctcGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAatat > 1:1019150/1‑71 (MQ=255) ctcGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAatat > 1:2235491/1‑71 (MQ=255) ctcGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAatat > 1:1910082/1‑71 (MQ=255) ctcGTTTTCCTTAACCATGTTACATAGAATGTGAACGGAAATTTAACCTGCCTCATATTTGGAGCAAata > 1:3430787/1‑70 (MQ=255) tcGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATg > 1:1656254/1‑71 (MQ=255) gTTTTCCTTAACCATGTTACATAGAATGTGCACTGAAATTTAACCTGCCTCATATTTGCTCCAAATATg > 1:4074237/1‑69 (MQ=38) gTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGCTCCAAATATg > 1:989239/1‑69 (MQ=39) gTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGCTCCAAATATg > 1:121027/1‑69 (MQ=39) gTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGCTCCAAATATg > 1:86340/1‑69 (MQ=39) gTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGCTCCAAATATg > 1:850263/1‑69 (MQ=39) gTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGCTCCAAATATg > 1:695280/1‑69 (MQ=39) gTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCCCATATTTGCTCCAAATATg < 1:3925500/69‑1 (MQ=38) ttttCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAata < 1:2610586/66‑1 (MQ=255) ttttCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAata < 1:3061810/66‑1 (MQ=255) ttttCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAata < 1:1827983/66‑1 (MQ=255) ttCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGAcc > 1:2894848/1‑70 (MQ=255) cTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGt > 1:2002500/1‑71 (MQ=255) cTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGt < 1:2521939/71‑1 (MQ=255) cTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGt > 1:21733/1‑71 (MQ=255) cTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGt > 1:3676703/1‑71 (MQ=255) tAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGCTCCAAATATga > 1:419490/1‑61 (MQ=38) tAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGCTCCAAATATga > 1:3973238/1‑61 (MQ=38) tAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGCTCCAAATATga > 1:1519227/1‑61 (MQ=38) cATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCa < 1:2328827/71‑1 (MQ=255) tGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAtt < 1:3770527/71‑1 (MQ=255) tGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAtt > 1:1176157/1‑71 (MQ=255) tGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAtt > 1:3025195/1‑71 (MQ=255) tGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAtt > 1:3507842/1‑71 (MQ=255) gTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCa < 1:3684721/48‑1 (MQ=255) ttACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTc < 1:3386323/66‑1 (MQ=255) ttACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTc < 1:2957669/66‑1 (MQ=255) aCATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACcgc > 1:2679542/1‑57 (MQ=255) aCATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTg > 1:1640304/1‑70 (MQ=255) aCATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTg < 1:4038906/70‑1 (MQ=255) aCATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTg > 1:1476865/1‑70 (MQ=255) aCATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTg > 1:4232342/1‑70 (MQ=255) cATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTg < 1:2928263/69‑1 (MQ=255) tAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACt < 1:2924773/71‑1 (MQ=255) tAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACt < 1:3892171/71‑1 (MQ=255) gAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa > 1:3365366/1‑42 (MQ=255) gAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa > 1:3360551/1‑42 (MQ=255) gAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTgg < 1:2433866/71‑1 (MQ=255) gAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTgg < 1:2872320/71‑1 (MQ=255) gAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTgg < 1:1290720/71‑1 (MQ=255) tgtgCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCAc > 1:4136176/1‑71 (MQ=255) tgCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACtt > 1:4138001/1‑71 (MQ=255) tgCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACtt > 1:2043133/1‑71 (MQ=255) tgCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACt < 1:1350270/70‑1 (MQ=255) tgCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGAACtt > 1:640163/1‑71 (MQ=255) cACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGc < 1:3398738/71‑1 (MQ=255) gAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCgtt < 1:2279820/71‑1 (MQ=255) gAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCgtt < 1:2934589/71‑1 (MQ=255) ttAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGc > 1:2875132/1‑71 (MQ=255) tAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAttt > 1:2842771/1‑46 (MQ=255) tAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCa > 1:1361914/1‑56 (MQ=255) tAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCgttg > 1:255924/1‑66 (MQ=255) tAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCgttg > 1:3989994/1‑66 (MQ=255) tAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCgt > 1:3230253/1‑64 (MQ=255) aCCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGAt < 1:1896304/71‑1 (MQ=255) ccTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGttgttg > 1:3989342/1‑66 (MQ=255) ccTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGttgttg > 1:1508081/1‑66 (MQ=255) gCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTg < 1:916055/42‑1 (MQ=255) gCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTg < 1:1642911/42‑1 (MQ=255) gCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTg < 1:3708838/42‑1 (MQ=255) gCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATtctc > 1:1738559/1‑71 (MQ=255) gCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATtct > 1:4210340/1‑70 (MQ=255) ccTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCg < 1:1607308/71‑1 (MQ=255) ccTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCg < 1:774015/71‑1 (MQ=255) ccTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCg < 1:4210788/71‑1 (MQ=255) ccTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCg < 1:3245737/71‑1 (MQ=255) cTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTa > 1:1629673/1‑42 (MQ=255) atatTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTgg < 1:3500147/43‑1 (MQ=255) atatTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTgg < 1:1371455/43‑1 (MQ=255) atatTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGc < 1:42195/68‑1 (MQ=255) atatTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGc < 1:2268572/68‑1 (MQ=255) atatTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCAct < 1:1410649/71‑1 (MQ=255) atatTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCAct < 1:4088205/71‑1 (MQ=255) atatTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCAct < 1:1690268/71‑1 (MQ=255) tatTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCgttgtt > 1:2438439/1‑56 (MQ=255) tatTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATtc < 1:3160377/63‑1 (MQ=255) tatTTGGAGCAAATATGGAACGCGTCCTTCATTTTGTACTGGCACTTGCCgttgtt > 1:3055744/1‑56 (MQ=255) atTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCa > 1:1046757/1‑43 (MQ=255) atTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCa > 1:868570/1‑43 (MQ=255) atTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCa > 1:859236/1‑43 (MQ=255) atTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCa > 1:316912/1‑43 (MQ=255) atTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCa > 1:3519156/1‑43 (MQ=255) tttGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATtctc > 1:590003/1‑63 (MQ=255) tttGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATtctc > 1:3567241/1‑63 (MQ=255) tttGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCActgct > 1:2149921/1‑71 (MQ=255) tGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGa > 1:2373642/1‑56 (MQ=255) gAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTa < 1:186628/71‑1 (MQ=255) | CAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTA > minE/1569587‑1569723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |