Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,697,632	A→G	44.7%	Y195C (TAT→TGT)	yfhR →	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,697,632	0	A	G	44.7%	6.3 / 51.3	38	Y195C (TAT→TGT)	yfhR	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base A (8/13);  new base G (5/12);  total (13/25)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

TGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAGCGGCG  >  minE/1697567‑1697697                                                                  |                                                                  tGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCaa                                                              >  1:2408454/1‑71 (MQ=255)             gggTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacc                                                  <  1:3009550/71‑1 (MQ=255)             gggTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacc                                                  <  1:2887320/71‑1 (MQ=255)              ggTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca                                                 <  1:3042614/71‑1 (MQ=255)              ggTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca                                                 <  1:991610/71‑1 (MQ=255)              ggTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca                                                 <  1:3506011/71‑1 (MQ=255)               gTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca                                                 >  1:684319/1‑70 (MQ=255)                   tCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGa                                            <  1:2381184/71‑1 (MQ=255)                   tCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGa                                            <  1:2886660/71‑1 (MQ=255)                    cGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa                                                                   >  1:225866/1‑47 (MQ=255)                       gAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATccc                                        >  1:1520769/1‑71 (MQ=255)                          ggCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCgg                                     <  1:1444109/71‑1 (MQ=255)                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                    <  1:4129377/71‑1 (MQ=255)                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                    <  1:3524193/71‑1 (MQ=255)                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                    <  1:2087977/71‑1 (MQ=255)                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                    <  1:1907829/71‑1 (MQ=255)                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTgg                               >  1:966497/1‑71 (MQ=255)                                      gTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCaaa                                               >  1:760526/1‑49 (MQ=255)                                      gTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCaaa                                               >  1:2848382/1‑49 (MQ=255)                                         aTCCTCGACTCCACATTTGCATCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCtacttact                      >  1:959304/1‑71 (MQ=255)                                           ccTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATACCCGGCAGTGGCTacttactt                     >  1:1049593/1‑70 (MQ=255)                                            cTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtga                   <  1:2240712/71‑1 (MQ=255)                                            cTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtga                   <  1:2080611/71‑1 (MQ=255)                                            cTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGAT‑CCCGGCAGTGGCTACTTACTtga                   <  1:170120/70‑1 (MQ=255)                                              cGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgat                  >  1:1136386/1‑70 (MQ=255)                                               gACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgatga                <  1:2577833/71‑1 (MQ=255)                                               gACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgatga                <  1:985923/71‑1 (MQ=255)                                               gACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgatga                <  1:2800006/71‑1 (MQ=255)                                               gACTCCACATTTGCCTCTTATGCAACCACCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgatga                <  1:1490264/71‑1 (MQ=255)                                                    cacaTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTTATGAGAGt            >  1:2123305/1‑70 (MQ=255)                                                      caTTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTAc         <  1:2768889/71‑1 (MQ=255)                                                      caTTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTAc         <  1:2586465/71‑1 (MQ=255)                                                      caTTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTAc         <  1:1568388/71‑1 (MQ=255)                                                      caTTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTAc         <  1:1486640/71‑1 (MQ=255)                                                      caTTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTAc         <  1:1475798/71‑1 (MQ=255)                                                       aTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgat                  <  1:2660567/61‑1 (MQ=255)                                                        tttGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAg       >  1:403235/1‑71 (MQ=255)                                                        tttGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAg       >  1:1796707/1‑71 (MQ=255)                                                                ttATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAgcggcg  >  1:1364299/1‑68 (MQ=255)                                                                  |                                                                  TGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAGCGGCG  >  minE/1697567‑1697697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑