| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,747,876 | G→A | 26.2% | S44L (TCA→TTA) | yfiE ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,747,876 | 0 | G | A | 26.2% | 109.7 / 39.7 | 61 | S44L (TCA→TTA) | yfiE | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (19/26); new base A (12/4); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGC > minE/1747814‑1747941 | gcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTcc < 1:1566472/71‑1 (MQ=255)gcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTcc < 1:1562609/71‑1 (MQ=255) aaCTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTaaa < 1:2125771/63‑1 (MQ=255) cTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCt > 1:85650/1‑71 (MQ=255) cTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCt > 1:2199104/1‑71 (MQ=255) tttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTc > 1:2079525/1‑70 (MQ=255) tttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTc > 1:4146769/1‑70 (MQ=255) ttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTc > 1:1023725/1‑69 (MQ=255) ttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTc > 1:1630154/1‑69 (MQ=255) ttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTc > 1:4019832/1‑69 (MQ=255) ttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTc > 1:593259/1‑69 (MQ=255) ttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTc > 1:3716348/1‑69 (MQ=255) ttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTc > 1:3591287/1‑69 (MQ=255) gTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAatat > 1:4136570/1‑70 (MQ=255) gTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCa < 1:1615478/57‑1 (MQ=255) gTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCa < 1:232114/57‑1 (MQ=255) tAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATg > 1:2639351/1‑70 (MQ=255) tAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATg > 1:2671165/1‑70 (MQ=255) tAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATg > 1:1066526/1‑70 (MQ=255) aGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGaa > 1:2200539/1‑70 (MQ=255) aGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGaa > 1:3202941/1‑70 (MQ=255) aGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGaa > 1:1159041/1‑70 (MQ=255) caTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:1490982/70‑1 (MQ=255) aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:2107260/36‑1 (MQ=255) aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:2375018/36‑1 (MQ=255) aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:3956817/36‑1 (MQ=255) aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:3407432/36‑1 (MQ=255) tCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:4023709/35‑1 (MQ=255) tCCGTCGGCCAATTTTCTCAAATAACTGGACTGGAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAcc > 1:2757661/1‑71 (MQ=255) tCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAcc > 1:3862636/1‑71 (MQ=255) tCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAcc > 1:3758579/1‑71 (MQ=255) tCCGTCGGCCAATTTTCTCAAATAACAGGACTGaaaa > 1:1720287/1‑37 (MQ=255) cGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGt > 1:3972795/1‑71 (MQ=255) gTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTc < 1:2720507/71‑1 (MQ=255) tCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGaa < 1:3507795/59‑1 (MQ=255) tCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCg < 1:322633/71‑1 (MQ=255) tCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCg < 1:327645/71‑1 (MQ=255) ccAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtg < 1:935540/71‑1 (MQ=255) ccAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtg < 1:4231648/71‑1 (MQ=255) aaTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:3583042/71‑1 (MQ=255) aaTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:2187576/71‑1 (MQ=255) aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:3361362/70‑1 (MQ=255) aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:2003396/70‑1 (MQ=255) aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:4187869/70‑1 (MQ=255) ttttCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTa > 1:2942267/1‑71 (MQ=255) tttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTa > 1:2710059/1‑70 (MQ=255) ttCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:1242188/67‑1 (MQ=255) ttCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:2844874/67‑1 (MQ=255) ttCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTa > 1:3851266/1‑69 (MQ=255) ctcAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGa > 1:130127/1‑46 (MQ=255) ctcAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGt > 1:617569/1‑51 (MQ=255) ctcAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGt > 1:1971556/1‑51 (MQ=255) tcAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGaa < 1:1156842/40‑1 (MQ=255) tcAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGaa < 1:1292853/40‑1 (MQ=255) aaataaatGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGcac > 1:6594/1‑70 (MQ=255) aaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATGTGAAAAGTCACCGTc < 1:1430970/55‑1 (MQ=255) aaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTc < 1:799501/55‑1 (MQ=255) aaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGcac > 1:2125335/1‑70 (MQ=255) aTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGt < 1:2999274/46‑1 (MQ=255) gACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGc > 1:3111224/1‑70 (MQ=255) tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:4152240/50‑1 (MQ=255) tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:2624720/50‑1 (MQ=255) gAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:48626/49‑1 (MQ=255) | GCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGC > minE/1747814‑1747941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |