Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,803,598 T→C 27.0% N30N (AAT→AAC ygaH → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,803,5980TC27.0% 101.8 / 45.7 63N30N (AAT→AACygaHpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (35/11);  new base C (12/5);  total (47/16)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATG  >  minE/1803529‑1803663
                                                                     |                                                                 
gctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATg                                                                  >  1:2813094/1‑71 (MQ=255)
 ctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAAt                                                                   <  1:2367399/69‑1 (MQ=255)
 ctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATgc                                                                 >  1:2618832/1‑71 (MQ=255)
 ctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATgc                                                                 >  1:3278993/1‑71 (MQ=255)
         aCTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCccaa                                                         >  1:3681226/1‑71 (MQ=255)
         aCTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCccaa                                                         >  1:1777868/1‑71 (MQ=255)
         aCTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCcca                                                          <  1:2671389/70‑1 (MQ=255)
         aCTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAACCCCCGCcca                                                          <  1:2176575/70‑1 (MQ=255)
            aGTTGGGATGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCccaacc                                                       >  1:2413408/1‑70 (MQ=255)
            aGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCccaacc                                                       >  1:1396472/1‑70 (MQ=255)
            aGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCccaacc                                                       >  1:624338/1‑70 (MQ=255)
            aGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCccaacc                                                       >  1:769973/1‑70 (MQ=255)
             gTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCccaacca                                                      <  1:453338/70‑1 (MQ=255)
                   gtggcgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAAt                                                                   >  1:761994/1‑51 (MQ=255)
                   gtggcgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTgg                                               >  1:2717712/1‑71 (MQ=255)
                      gcgAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGgcgc                                            >  1:2397331/1‑71 (MQ=255)
                          attattGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCCCAACCAAACGTGGCGCgg                                          >  1:1466156/1‑69 (MQ=255)
                          attattGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCCCAACCAAACGTGGCGCgg                                          >  1:2673749/1‑69 (MQ=255)
                           ttattGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggt                                         <  1:2601977/69‑1 (MQ=255)
                             attGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggtagg                                      >  1:230516/1‑70 (MQ=255)
                             attGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggtagg                                      >  1:1002209/1‑70 (MQ=255)
                             attGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggtagg                                      >  1:1279984/1‑70 (MQ=255)
                             attGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggtag                                       >  1:635644/1‑69 (MQ=255)
                             attGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggtag                                       >  1:3388354/1‑69 (MQ=255)
                             attGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggtag                                       >  1:1863819/1‑69 (MQ=255)
                                 cTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTAtt                                  <  1:526515/70‑1 (MQ=255)
                                 cTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTAtt                                  <  1:2270925/70‑1 (MQ=255)
                                 cTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTAtt                                  <  1:3842258/70‑1 (MQ=255)
                                    ccGCTATTTGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTg                               <  1:1634657/70‑1 (MQ=255)
                                            tGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGacac                       <  1:3373903/70‑1 (MQ=255)
                                            tGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCccaaccaa                                                     >  1:1004964/1‑40 (MQ=255)
                                            tGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCCCAACCAAACGTg                                                >  1:411160/1‑45 (MQ=255)
                                            tGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCCCAACCAAACGTg                                                >  1:1915537/1‑45 (MQ=255)
                                            tGCCGCTGCGCCTGCGTGTGGGTAACGCCCGCCCAACCAAACGTg                                                >  1:2040933/1‑45 (MQ=255)
                                                 cTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGac                         >  1:3944930/1‑63 (MQ=255)
                                                 cTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGac                         >  1:2741128/1‑63 (MQ=255)
                                                 cTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGac                         >  1:2387401/1‑63 (MQ=255)
                                                 cTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGac                         >  1:2310830/1‑63 (MQ=255)
                                                   gcgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCa               >  1:1218422/1‑71 (MQ=255)
                                                   gcgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCa               >  1:3155571/1‑71 (MQ=255)
                                                   gcgcCTGCGTGTGGGTAATGCCCGCCCAACAAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCa               >  1:2782356/1‑71 (MQ=255)
                                                   gcgcCTGCGTGTGGGTAATGCCCGCACAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCa               >  1:341284/1‑71 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTAtt                                  >  1:3372409/1‑51 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTAtt                                  >  1:3731772/1‑51 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTAtt                                  >  1:207673/1‑51 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTAtt                                  >  1:3029468/1‑51 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTAtt                                  >  1:1112967/1‑51 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTAtt                                  >  1:812034/1‑51 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGc                <  1:2628314/69‑1 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGc                <  1:651178/69‑1 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACAATTGGc                <  1:2709/69‑1 (MQ=255)
                                                    cgcCTGCGTGTGGGTAATGCACGCCCAAGCAAACGTGGCGCGGTAGGTAtt                                  >  1:3278535/1‑51 (MQ=255)
                                                         gCGTGTTGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCAACACCATTGGCATCTCCt         >  1:2413179/1‑71 (MQ=255)
                                                             gtgGGTAACGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGa      >  1:380168/1‑70 (MQ=255)
                                                              tgGGTAACGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGata    <  1:3660349/71‑1 (MQ=255)
                                                              tgGGTAACGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGata    <  1:686216/71‑1 (MQ=255)
                                                               gggTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGata    >  1:3652976/1‑70 (MQ=255)
                                                                ggTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGatat   >  1:1477631/1‑70 (MQ=255)
                                                                ggTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGatat   >  1:2991361/1‑70 (MQ=255)
                                                                ggTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATg  >  1:251361/1‑71 (MQ=255)
                                                                ggTAACGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATg  >  1:2507629/1‑71 (MQ=255)
                                                                 gTAACGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATg  >  1:1379595/1‑70 (MQ=255)
                                                                 gTAACGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATg  >  1:2911837/1‑70 (MQ=255)
                                                                  tAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCa                     <  1:1375398/50‑1 (MQ=255)
                                                                  tAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCa                     <  1:1061006/50‑1 (MQ=255)
                                                                     |                                                                 
GCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATG  >  minE/1803529‑1803663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: