| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,807,377 | T→C | 26.1% | intergenic (+58/+6) | emrB → / ← luxS | multidrug efflux system protein/S‑ribosylhomocysteinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,807,377 | 0 | T | C | 26.1% | 117.5 / 47.8 | 69 | intergenic (+58/+6) | emrB/luxS | multidrug efflux system protein/S‑ribosylhomocysteinase |
| Reads supporting (aligned to +/- strand): ref base T (27/24); new base C (14/4); total (41/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.43e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATG > minE/1807310‑1807443 | tGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt < 1:2417971/71‑1 (MQ=255)tGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt < 1:1426814/71‑1 (MQ=255)tGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt < 1:4036045/71‑1 (MQ=255) gcACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc < 1:2208091/71‑1 (MQ=255) aCTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATACTTTACTGAc < 1:3628271/69‑1 (MQ=255) cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGAc > 1:953252/1‑68 (MQ=255) cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGAc > 1:2359204/1‑68 (MQ=255) cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGAc > 1:1438590/1‑68 (MQ=255) tAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc > 1:3177017/1‑67 (MQ=255) aGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtg > 1:335271/1‑71 (MQ=255) aGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtg > 1:3919249/1‑71 (MQ=255) tACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa < 1:2877760/58‑1 (MQ=255) tACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa < 1:3348831/58‑1 (MQ=255) tACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa < 1:1538395/58‑1 (MQ=255) tACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa < 1:3741353/58‑1 (MQ=255) aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGt < 1:304204/71‑1 (MQ=255) aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGt < 1:566316/71‑1 (MQ=255) aaGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGttt > 1:672864/1‑50 (MQ=255) aaGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:1768583/1‑51 (MQ=255) aaGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc > 1:1891980/1‑71 (MQ=255) aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCt < 1:1050447/71‑1 (MQ=255) gCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTAc < 1:1816314/50‑1 (MQ=255) gCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTAc < 1:4168711/50‑1 (MQ=255) aGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc < 1:2403578/69‑1 (MQ=255) gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc < 1:2238706/68‑1 (MQ=255) ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTa < 1:2559358/51‑1 (MQ=255) ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTa < 1:2564416/51‑1 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:2336549/1‑43 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGtt > 1:901791/1‑60 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:3347854/1‑71 (MQ=255) ttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTctct > 1:937685/1‑71 (MQ=255) ttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTctct > 1:891820/1‑71 (MQ=255) ttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTctct > 1:3377037/1‑71 (MQ=255) tggaCTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACATCTCtt > 1:1823234/4‑71 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt > 1:3569554/1‑71 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt > 1:352306/1‑71 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt > 1:533842/1‑71 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACATCTCtt > 1:481012/1‑71 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACATCTCtt > 1:140831/1‑71 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACATCTCtt > 1:350817/1‑71 (MQ=255) gAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCttt > 1:1137668/1‑71 (MQ=255) gAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCttt > 1:3232815/1‑71 (MQ=255) gAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCttt > 1:2378565/1‑71 (MQ=255) gAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt < 1:568194/70‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg < 1:4171504/71‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg < 1:2608529/71‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg < 1:1511890/71‑1 (MQ=255) tGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAAc < 1:3878751/59‑1 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc < 1:2646273/69‑1 (MQ=255) ttttttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCttt < 1:3235151/63‑1 (MQ=255) ttttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc > 1:3206830/1‑70 (MQ=255) ttttCAATTAATTGTGAAGATAGTTCACTGACTAGa > 1:1362336/1‑36 (MQ=255) ttttCAATTAATTGTGAAGATAGTTCACTGACTAGa > 1:4242613/1‑36 (MQ=255) tttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc > 1:2396589/1‑69 (MQ=255) tCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGt > 1:953896/1‑71 (MQ=255) tCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGt > 1:1869143/1‑71 (MQ=255) aattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg > 1:632890/1‑71 (MQ=255) aattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg > 1:3744648/1‑71 (MQ=255) aattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg > 1:3776916/1‑71 (MQ=255) aattGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg > 1:422797/1‑71 (MQ=255) aattGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg > 1:369658/1‑71 (MQ=255) aattGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg > 1:1388646/1‑71 (MQ=255) attGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg < 1:2867478/70‑1 (MQ=255) attGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg < 1:572303/70‑1 (MQ=255) tgtgAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAg > 1:2413794/1‑55 (MQ=255) tgtgAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAg > 1:3717668/1‑55 (MQ=255) tgtgAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTg > 1:2745274/1‑71 (MQ=255) tgtgAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTg > 1:2209732/1‑71 (MQ=255) aaGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg < 1:1887948/70‑1 (MQ=255) aGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATg > 1:120235/1‑71 (MQ=255) | TGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATG > minE/1807310‑1807443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |